preprint draft Katarina Tluckova author 4AC7D980-F248-11E8-B48F-1D18A9856A87 Anita P Testa Salmazo author 41F1F098-F248-11E8-B48F-1D18A9856A87 Carrie A Bernecky author 2CB9DFE2-F248-11E8-B48F-1D18A9856A870000-0003-0893-7036 Regulation of mammalian transcription by noncoding RNA project Transcription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here we present cryo-electron microscopy structures of Pol II bound to Alu RNA, which reveal that Alu RNA mimics how DNA and RNA bind to Pol II during transcription elongation. Further, we show how domains of the general transcription factor TFIIF affect complex dynamics and control repressive activity. Together, we reveal how a non-coding RNA can regulate mammalian gene expression. https://research-explorer.ista.ac.at/download/14644/14646/2023_Tluckova_etal_REx.pdf application/pdfno https://creativecommons.org/licenses/by-nc/4.0/ Institute of Science and Technology Austria2023 eng 10.15479/AT:ISTA:14644 https://research-explorer.ista.ac.at/record/18778 Tluckova, Katarina, Anita P Testa Salmazo, and Carrie Bernecky. “Mechanism of Mammalian Transcriptional Repression by Noncoding RNA.” Institute of Science and Technology Austria, n.d. <a href="https://doi.org/10.15479/AT:ISTA:14644">https://doi.org/10.15479/AT:ISTA:14644</a>. Tluckova K, Testa Salmazo AP, Bernecky C. Mechanism of mammalian transcriptional repression by noncoding RNA. doi:<a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a> K. Tluckova, A. P. Testa Salmazo, and C. Bernecky, “Mechanism of mammalian transcriptional repression by noncoding RNA.” Institute of Science and Technology Austria. Tluckova, K., Testa Salmazo, A. P., &#38; Bernecky, C. (n.d.). Mechanism of mammalian transcriptional repression by noncoding RNA. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:14644">https://doi.org/10.15479/AT:ISTA:14644</a> Tluckova K, Testa Salmazo AP, Bernecky C. Mechanism of mammalian transcriptional repression by noncoding RNA. <a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a>. Tluckova, Katarina, et al. <i>Mechanism of Mammalian Transcriptional Repression by Noncoding RNA</i>. Institute of Science and Technology Austria, doi:<a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a>. K. Tluckova, A.P. Testa Salmazo, C. Bernecky, (n.d.). 146442023-12-04T14:51:00Z2025-11-20T10:28:37Z