---
_id: '14683'
abstract:
- lang: eng
  text: "Mosaic analysis with double markers (MADM) technology enables the generation
    of genetic mosaic tissue in mice and high-resolution phenotyping at the individual
    cell level. Here, we present a protocol for isolating MADM-labeled cells with
    high yield for downstream molecular analyses using fluorescence-activated cell
    sorting (FACS). We describe steps for generating MADM-labeled mice, perfusion,
    single-cell suspension, and debris removal. We then detail procedures for cell
    sorting by FACS and downstream analysis. This protocol is suitable for embryonic
    to adult mice.\r\nFor complete details on the use and execution of this protocol,
    please refer to Contreras et al. (2021).1"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: This research was supported by the Scientific Service Units (SSU)
  at IST Austria through resources provided by the Imaging & Optics Facility (IOF)
  and Preclinical Facilities (PCF). N.A. received support from FWF Firnberg-Programme
  (T 1031). G.C. received support from the European Union’s Horizon 2020 research
  and innovation programme under the Marie Skłodowska-Curie grant agreement no. 754411
  as an ISTplus postdoctoral fellow. This work was also supported by IST Austria institutional
  funds, FWF SFB F78 to S.H., and the European Research Council (ERC) under the European
  Union’s Horizon 2020 research and innovation programme (grant agreement no. 725780
  LinPro) to S.H.
article_number: '102771'
article_processing_charge: Yes (in subscription journal)
article_type: review
author:
- first_name: Nicole
  full_name: Amberg, Nicole
  id: 4CD6AAC6-F248-11E8-B48F-1D18A9856A87
  last_name: Amberg
  orcid: 0000-0002-3183-8207
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Amberg N, Cheung GT, Hippenmeyer S. Protocol for sorting cells from mouse brains
    labeled with mosaic analysis with double markers by flow cytometry. <i>STAR Protocols</i>.
    2024;5(1). doi:<a href="https://doi.org/10.1016/j.xpro.2023.102771">10.1016/j.xpro.2023.102771</a>
  apa: Amberg, N., Cheung, G. T., &#38; Hippenmeyer, S. (2024). Protocol for sorting
    cells from mouse brains labeled with mosaic analysis with double markers by flow
    cytometry. <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2023.102771">https://doi.org/10.1016/j.xpro.2023.102771</a>
  chicago: Amberg, Nicole, Giselle T Cheung, and Simon Hippenmeyer. “Protocol for
    Sorting Cells from Mouse Brains Labeled with Mosaic Analysis with Double Markers
    by Flow Cytometry.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2023.102771">https://doi.org/10.1016/j.xpro.2023.102771</a>.
  ieee: N. Amberg, G. T. Cheung, and S. Hippenmeyer, “Protocol for sorting cells from
    mouse brains labeled with mosaic analysis with double markers by flow cytometry,”
    <i>STAR Protocols</i>, vol. 5, no. 1. Elsevier, 2024.
  ista: Amberg N, Cheung GT, Hippenmeyer S. 2024. Protocol for sorting cells from
    mouse brains labeled with mosaic analysis with double markers by flow cytometry.
    STAR Protocols. 5(1), 102771.
  mla: Amberg, Nicole, et al. “Protocol for Sorting Cells from Mouse Brains Labeled
    with Mosaic Analysis with Double Markers by Flow Cytometry.” <i>STAR Protocols</i>,
    vol. 5, no. 1, 102771, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xpro.2023.102771">10.1016/j.xpro.2023.102771</a>.
  short: N. Amberg, G.T. Cheung, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2023-12-13T11:48:05Z
date_published: 2024-03-15T00:00:00Z
date_updated: 2025-04-15T08:23:06Z
day: '15'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2023.102771
ec_funded: 1
external_id:
  pmid:
  - '38070137'
file:
- access_level: open_access
  checksum: 3f0ee62e04bf5a44b45b035662826e95
  content_type: application/pdf
  creator: dernst
  date_created: 2024-07-16T11:50:03Z
  date_updated: 2024-07-16T11:50:03Z
  file_id: '17260'
  file_name: 2024_STARProtoc_Amberg.pdf
  file_size: 8871807
  relation: main_file
  success: 1
file_date_updated: 2024-07-16T11:50:03Z
has_accepted_license: '1'
intvolume: '         5'
issue: '1'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- General Neuroscience
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 268F8446-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: T01031
  name: Role of Eed in neural stem cell lineage progression
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
- _id: 260018B0-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '725780'
  name: Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development
publication: STAR Protocols
publication_identifier:
  issn:
  - 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for sorting cells from mouse brains labeled with mosaic analysis with
  double markers by flow cytometry
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...