LIONESS enables 4D nanoscale reconstruction of living brain tissue

article LIONESS enables 4D nanoscale reconstruction of living brain tissue published yes Johann G Danzl author 42EFD3B6-F248-11E8-B48F-1D18A9856A870000-0001-8559-3973 Philipp Velicky author 39BDC62C-F248-11E8-B48F-1D18A9856A870000-0002-2340-7431 JoDa department We developed LIONESS, a technology that leverages improvements to optical super-resolution microscopy and prior information on sample structure via machine learning to overcome the limitations (in 3D-resolution, signal-to-noise ratio and light exposure) of optical microscopy of living biological specimens. LIONESS enables dense reconstruction of living brain tissue and morphodynamics visualization at the nanoscale. Springer Nature2023 eng Cell BiologyMolecular BiologyBiochemistryBiotechnology Nature Methods 1548-7091 1548-7105 00102562150000210.1038/s41592-023-01937-5 2081141-1142 https://research-explorer.ista.ac.at/record/13267 Danzl JG, Velicky P. LIONESS enables 4D nanoscale reconstruction of living brain tissue. Nature Methods. 2023;20(8):1141-1142. doi:<a href="https://doi.org/10.1038/s41592-023-01937-5">10.1038/s41592-023-01937-5</a> Danzl, Johann G, and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction of Living Brain Tissue.” Nature Methods. Springer Nature, 2023. <a href="https://doi.org/10.1038/s41592-023-01937-5">https://doi.org/10.1038/s41592-023-01937-5</a>. Danzl JG, Velicky P. 2023. LIONESS enables 4D nanoscale reconstruction of living brain tissue. Nature Methods. 20(8), 1141–1142. J.G. Danzl, P. Velicky, Nature Methods 20 (2023) 1141–1142. Danzl, J. G., & Velicky, P. (2023). LIONESS enables 4D nanoscale reconstruction of living brain tissue. Nature Methods. Springer Nature. <a href="https://doi.org/10.1038/s41592-023-01937-5">https://doi.org/10.1038/s41592-023-01937-5</a> Danzl, Johann G., and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction of Living Brain Tissue.” Nature Methods, vol. 20, no. 8, Springer Nature, 2023, pp. 1141–42, doi:<a href="https://doi.org/10.1038/s41592-023-01937-5">10.1038/s41592-023-01937-5</a>. J. G. Danzl and P. Velicky, “LIONESS enables 4D nanoscale reconstruction of living brain tissue,” Nature Methods, vol. 20, no. 8. Springer Nature, pp. 1141–1142, 2023. 147702024-01-10T08:07:15Z2026-04-14T08:34:34Z