---
_id: '14770'
abstract:
- lang: eng
  text: We developed LIONESS, a technology that leverages improvements to optical
    super-resolution microscopy and prior information on sample structure via machine
    learning to overcome the limitations (in 3D-resolution, signal-to-noise ratio
    and light exposure) of optical microscopy of living biological specimens. LIONESS
    enables dense reconstruction of living brain tissue and morphodynamics visualization
    at the nanoscale.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Johann G
  full_name: Danzl, Johann G
  id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
  last_name: Danzl
  orcid: 0000-0001-8559-3973
- first_name: Philipp
  full_name: Velicky, Philipp
  id: 39BDC62C-F248-11E8-B48F-1D18A9856A87
  last_name: Velicky
  orcid: 0000-0002-2340-7431
citation:
  ama: Danzl JG, Velicky P. LIONESS enables 4D nanoscale reconstruction of living
    brain tissue. <i>Nature Methods</i>. 2023;20(8):1141-1142. doi:<a href="https://doi.org/10.1038/s41592-023-01937-5">10.1038/s41592-023-01937-5</a>
  apa: Danzl, J. G., &#38; Velicky, P. (2023). LIONESS enables 4D nanoscale reconstruction
    of living brain tissue. <i>Nature Methods</i>. Springer Nature. <a href="https://doi.org/10.1038/s41592-023-01937-5">https://doi.org/10.1038/s41592-023-01937-5</a>
  chicago: Danzl, Johann G, and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction
    of Living Brain Tissue.” <i>Nature Methods</i>. Springer Nature, 2023. <a href="https://doi.org/10.1038/s41592-023-01937-5">https://doi.org/10.1038/s41592-023-01937-5</a>.
  ieee: J. G. Danzl and P. Velicky, “LIONESS enables 4D nanoscale reconstruction of
    living brain tissue,” <i>Nature Methods</i>, vol. 20, no. 8. Springer Nature,
    pp. 1141–1142, 2023.
  ista: Danzl JG, Velicky P. 2023. LIONESS enables 4D nanoscale reconstruction of
    living brain tissue. Nature Methods. 20(8), 1141–1142.
  mla: Danzl, Johann G., and Philipp Velicky. “LIONESS Enables 4D Nanoscale Reconstruction
    of Living Brain Tissue.” <i>Nature Methods</i>, vol. 20, no. 8, Springer Nature,
    2023, pp. 1141–42, doi:<a href="https://doi.org/10.1038/s41592-023-01937-5">10.1038/s41592-023-01937-5</a>.
  short: J.G. Danzl, P. Velicky, Nature Methods 20 (2023) 1141–1142.
corr_author: '1'
date_created: 2024-01-10T08:07:15Z
date_published: 2023-08-01T00:00:00Z
date_updated: 2026-04-14T08:34:34Z
day: '01'
department:
- _id: JoDa
doi: 10.1038/s41592-023-01937-5
external_id:
  isi:
  - '001025621500002'
intvolume: '        20'
isi: 1
issue: '8'
keyword:
- Cell Biology
- Molecular Biology
- Biochemistry
- Biotechnology
language:
- iso: eng
month: '08'
oa_version: None
page: 1141-1142
publication: Nature Methods
publication_identifier:
  eissn:
  - 1548-7105
  issn:
  - 1548-7091
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  record:
  - id: '13267'
    relation: extended_version
    status: public
scopus_import: '1'
status: public
title: LIONESS enables 4D nanoscale reconstruction of living brain tissue
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 20
year: '2023'
...