{"issue":"8","publication":"Cells","external_id":{"pmid":["37190042"],"isi":["000977445700001"]},"license":"https://creativecommons.org/licenses/by/4.0/","publisher":"MDPI","publication_status":"published","date_created":"2024-01-10T09:46:35Z","month":"04","file":[{"file_name":"2023_Cells_Cheung.pdf","checksum":"6798cd75d8857976fbc58a43fd173d68","relation":"main_file","file_id":"14808","content_type":"application/pdf","file_size":7931643,"success":1,"date_updated":"2024-01-16T09:26:52Z","access_level":"open_access","creator":"dernst","date_created":"2024-01-16T09:26:52Z"}],"_id":"14783","article_type":"original","status":"public","keyword":["General Medicine"],"type":"journal_article","date_updated":"2024-01-16T09:29:35Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"SiHi"}],"doi":"10.3390/cells12081133","oa_version":"Published Version","acknowledgement":"This research was funded by grants from the European Research Council (Consolidator grant #683154) and European Union’s Horizon 2020 research and innovation program (Marie Sklodowska-Curie Innovative Training Networks, grant #722053, EU-GliaPhD) to N.R., as well as from FP7-PEOPLE Marie Curie Intra-European Fellowship for career development (grant #622289) to G.C. We thank Elena Dossi, Grégory Ghézali, and Jérémie Teillon for support with setting up the MEA system for the two-photon microscope. We would also like to thank Tayfun Palaz for their technical assistance with the EM preparations.","file_date_updated":"2024-01-16T09:26:52Z","author":[{"full_name":"Cheung, Giselle T","id":"471195F6-F248-11E8-B48F-1D18A9856A87","first_name":"Giselle T","orcid":"0000-0001-8457-2572","last_name":"Cheung"},{"full_name":"Chever, Oana","last_name":"Chever","first_name":"Oana"},{"first_name":"Astrid","last_name":"Rollenhagen","full_name":"Rollenhagen, Astrid"},{"full_name":"Quenech’du, Nicole","last_name":"Quenech’du","first_name":"Nicole"},{"last_name":"Ezan","first_name":"Pascal","full_name":"Ezan, Pascal"},{"last_name":"Lübke","first_name":"Joachim H. R.","full_name":"Lübke, Joachim H. R."},{"full_name":"Rouach, Nathalie","first_name":"Nathalie","last_name":"Rouach"}],"language":[{"iso":"eng"}],"day":"11","year":"2023","abstract":[{"text":"Connexin 43, an astroglial gap junction protein, is enriched in perisynaptic astroglial processes and plays major roles in synaptic transmission. We have previously found that astroglial Cx43 controls synaptic glutamate levels and allows for activity-dependent glutamine release to sustain physiological synaptic transmissions and cognitiogns. However, whether Cx43 is important for the release of synaptic vesicles, which is a critical component of synaptic efficacy, remains unanswered. Here, using transgenic mice with a glial conditional knockout of Cx43 (Cx43−/−), we investigate whether and how astrocytes regulate the release of synaptic vesicles from hippocampal synapses. We report that CA1 pyramidal neurons and their synapses develop normally in the absence of astroglial Cx43. However, a significant impairment in synaptic vesicle distribution and release dynamics were observed. In particular, the FM1-43 assays performed using two-photon live imaging and combined with multi-electrode array stimulation in acute hippocampal slices, revealed a slower rate of synaptic vesicle release in Cx43−/− mice. Furthermore, paired-pulse recordings showed that synaptic vesicle release probability was also reduced and is dependent on glutamine supply via Cx43 hemichannel (HC). Taken together, we have uncovered a role for Cx43 in regulating presynaptic functions by controlling the rate and probability of synaptic vesicle release. Our findings further highlight the significance of astroglial Cx43 in synaptic transmission and efficacy.","lang":"eng"}],"oa":1,"isi":1,"pmid":1,"volume":12,"date_published":"2023-04-11T00:00:00Z","quality_controlled":"1","article_number":"1133","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"has_accepted_license":"1","publication_identifier":{"issn":["2073-4409"]},"ddc":["570"],"intvolume":"        12","citation":{"mla":"Cheung, Giselle T., et al. “Astroglial Connexin 43 Regulates Synaptic Vesicle Release at Hippocampal Synapses.” <i>Cells</i>, vol. 12, no. 8, 1133, MDPI, 2023, doi:<a href=\"https://doi.org/10.3390/cells12081133\">10.3390/cells12081133</a>.","ista":"Cheung GT, Chever O, Rollenhagen A, Quenech’du N, Ezan P, Lübke JHR, Rouach N. 2023. Astroglial connexin 43 regulates synaptic vesicle release at hippocampal synapses. Cells. 12(8), 1133.","ieee":"G. T. Cheung <i>et al.</i>, “Astroglial connexin 43 regulates synaptic vesicle release at hippocampal synapses,” <i>Cells</i>, vol. 12, no. 8. MDPI, 2023.","short":"G.T. Cheung, O. Chever, A. Rollenhagen, N. Quenech’du, P. Ezan, J.H.R. Lübke, N. Rouach, Cells 12 (2023).","apa":"Cheung, G. T., Chever, O., Rollenhagen, A., Quenech’du, N., Ezan, P., Lübke, J. H. R., &#38; Rouach, N. (2023). Astroglial connexin 43 regulates synaptic vesicle release at hippocampal synapses. <i>Cells</i>. MDPI. <a href=\"https://doi.org/10.3390/cells12081133\">https://doi.org/10.3390/cells12081133</a>","ama":"Cheung GT, Chever O, Rollenhagen A, et al. Astroglial connexin 43 regulates synaptic vesicle release at hippocampal synapses. <i>Cells</i>. 2023;12(8). doi:<a href=\"https://doi.org/10.3390/cells12081133\">10.3390/cells12081133</a>","chicago":"Cheung, Giselle T, Oana Chever, Astrid Rollenhagen, Nicole Quenech’du, Pascal Ezan, Joachim H. R. Lübke, and Nathalie Rouach. “Astroglial Connexin 43 Regulates Synaptic Vesicle Release at Hippocampal Synapses.” <i>Cells</i>. MDPI, 2023. <a href=\"https://doi.org/10.3390/cells12081133\">https://doi.org/10.3390/cells12081133</a>."},"title":"Astroglial connexin 43 regulates synaptic vesicle release at hippocampal synapses","article_processing_charge":"Yes"}