Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells

article Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells published yes Carlos J. Gallardo-Dodd author Christian Oertlin author Julien Record author Rômulo G. Galvani author Christian Sommerauer author Nikolai V. Kuznetsov author Evangelos Doukoumopoulos author Liaqat Ali author Mariana M. S. Oliveira author Christina Seitz author Mathias Percipalle author 4986e21c-eb97-11eb-a6c2-a4ef0b629971 Tijana Nikić author Anastasia A. Sadova author Sofia M. Shulgina author Vjacheslav A. Shmarov author Olga V. Kutko author Daria D. Vlasova author Kseniya D. Orlova author Marina P. Rykova author John Andersson author Piergiorgio Percipalle author Claudia Kutter author Sergey A. Ponomarev author Lisa S. Westerberg author FlSc department The next steps of deep space exploration are manned missions to Moon and Mars. For safe space missions for crew members, it is important to understand the impact of space flight on the immune system. We studied the effects of 21 days dry immersion (DI) exposure on the transcriptomes of T cells isolated from blood samples of eight healthy volunteers. Samples were collected 7 days before DI, at day 7, 14, and 21 during DI, and 7 days after DI. RNA sequencing of CD3+T cells revealed transcriptional alterations across all time points, with most changes occurring 14 days after DI exposure. At day 21, T cells showed evidence of adaptation with a transcriptional profile resembling that of 7 days before DI. At 7 days after DI, T cells again changed their transcriptional profile. These data suggest that T cells adapt by rewiring their transcriptomes in response to simulated weightlessness and that remodeling cues persist when reexposed to normal gravity. https://research-explorer.ista.ac.at/download/14784/14809/2023_ScienceAdvances_GallardoDodd.pdf application/pdfno American Association for the Advancement of Science2023 eng Multidisciplinary Science Advances 2375-2548 37624890 00105459680000710.1126/sciadv.adg1610 934 Gallardo-Dodd CJ, Oertlin C, Record J, et al. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. Science Advances. 2023;9(34). doi:<a href="https://doi.org/10.1126/sciadv.adg1610">10.1126/sciadv.adg1610</a> Gallardo-Dodd, Carlos J., et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” Science Advances, vol. 9, no. 34, adg1610, American Association for the Advancement of Science, 2023, doi:<a href="https://doi.org/10.1126/sciadv.adg1610">10.1126/sciadv.adg1610</a>. Gallardo-Dodd, Carlos J., Christian Oertlin, Julien Record, Rômulo G. Galvani, Christian Sommerauer, Nikolai V. Kuznetsov, Evangelos Doukoumopoulos, et al. “Exposure of Volunteers to Microgravity by Dry Immersion Bed over 21 Days Results in Gene Expression Changes and Adaptation of T Cells.” Science Advances. American Association for the Advancement of Science, 2023. <a href="https://doi.org/10.1126/sciadv.adg1610">https://doi.org/10.1126/sciadv.adg1610</a>. C.J. Gallardo-Dodd, C. Oertlin, J. Record, R.G. Galvani, C. Sommerauer, N.V. Kuznetsov, E. Doukoumopoulos, L. Ali, M.M.S. Oliveira, C. Seitz, M. Percipalle, T. Nikić, A.A. Sadova, S.M. Shulgina, V.A. Shmarov, O.V. Kutko, D.D. Vlasova, K.D. Orlova, M.P. Rykova, J. Andersson, P. Percipalle, C. Kutter, S.A. Ponomarev, L.S. Westerberg, Science Advances 9 (2023). Gallardo-Dodd, C. J., Oertlin, C., Record, J., Galvani, R. G., Sommerauer, C., Kuznetsov, N. V., … Westerberg, L. S. (2023). Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. Science Advances. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/sciadv.adg1610">https://doi.org/10.1126/sciadv.adg1610</a> Gallardo-Dodd CJ, Oertlin C, Record J, Galvani RG, Sommerauer C, Kuznetsov NV, Doukoumopoulos E, Ali L, Oliveira MMS, Seitz C, Percipalle M, Nikić T, Sadova AA, Shulgina SM, Shmarov VA, Kutko OV, Vlasova DD, Orlova KD, Rykova MP, Andersson J, Percipalle P, Kutter C, Ponomarev SA, Westerberg LS. 2023. Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells. Science Advances. 9(34), adg1610. C. J. Gallardo-Dodd et al., “Exposure of volunteers to microgravity by dry immersion bed over 21 days results in gene expression changes and adaptation of T cells,” Science Advances, vol. 9, no. 34. American Association for the Advancement of Science, 2023. 147842024-01-10T09:48:01Z2024-09-09T08:03:13Z