---
OA_place: repository
OA_type: green
_id: '14786'
abstract:
- lang: eng
  text: Acanthocephalans, intestinal parasites of vertebrates, are characterised by
    orders of magnitude higher metal accumulation than free-living organisms, but
    the mechanism of such effective metal accumulation is still unknown. The aim of
    our study was to gain new insights into the high-resolution localization of elements
    in the bodies of acanthocephalans, thus taking an initial step towards elucidating
    metal uptake and accumulation in organisms under real environmental conditions.
    For the first time, nanoscale secondary ion mass spectrometry (NanoSIMS) was used
    for high-resolution mapping of 12 elements (C, Ca, Cu, Fe, N, Na, O, P, Pb, S,
    Se, and Tl) in three selected body parts (trunk spines, inner part of the proboscis
    receptacle and inner surface of the tegument) of Dentitruncus truttae, a parasite
    of brown trout (Salmo trutta) from the Krka River in Croatia. In addition, the
    same body parts were examined using transmission electron microscopy (TEM) and
    correlated with NanoSIMS images. Metal concentrations determined using HR ICP-MS
    confirmed higher accumulation in D. truttae than in the fish intestine. The chemical
    composition of the acanthocephalan body showed the highest density of C, Ca, N,
    Na, O, S, as important and constitutive elements in living cells in all studied
    structures, while Fe was predominant among trace elements. In general, higher
    element density was found in trunk spines and tegument, as body structures responsible
    for substance absorption in parasites. The results obtained with NanoSIMS and
    TEM-NanoSIMS correlative imaging represent pilot data for mapping of elements
    at nanoscale resolution in the ultrastructure of various body parts of acanthocephalans
    and generally provide a contribution for further application of this technique
    in all parasite species.
acknowledgement: 'The authors thank the Czech Science Foundation (project No. 19-28399X)
  and the Czech Academy of Sciences (RVO: 60077344) and are sincerely grateful to
  the Bordeaux Imaging Centre (member of the France BioImaging national infrastructure,
  ANR-10-INBS-04) for help with TEM and to members of the Laboratory of Biological
  Effects of Metals and Laboratory of Aquaculture and Pathology of Aquatic Organisms
  (Ruđer Bošković Institute, Croatia) for the assistance with fieldwork.'
article_number: '164010'
article_processing_charge: No
article_type: original
author:
- first_name: Vlatka
  full_name: Filipović Marijić, Vlatka
  last_name: Filipović Marijić
- first_name: Maria Angels
  full_name: Subirana, Maria Angels
  last_name: Subirana
- first_name: Dirk
  full_name: Schaumlöffel, Dirk
  last_name: Schaumlöffel
- first_name: Josip
  full_name: Barišić, Josip
  last_name: Barišić
- first_name: Etienne
  full_name: Gontier, Etienne
  last_name: Gontier
- first_name: Nesrete
  full_name: Krasnici, Nesrete
  id: cb5852d4-287f-11ed-baf0-bc1dd2d5c745
  last_name: Krasnici
- first_name: Tatjana
  full_name: Mijošek, Tatjana
  last_name: Mijošek
- first_name: Jesús S.
  full_name: Hernández-Orts, Jesús S.
  last_name: Hernández-Orts
- first_name: Tomáš
  full_name: Scholz, Tomáš
  last_name: Scholz
- first_name: Marijana
  full_name: Erk, Marijana
  last_name: Erk
citation:
  ama: Filipović Marijić V, Subirana MA, Schaumlöffel D, et al. First insight in element
    localisation in different body parts of the acanthocephalan Dentitruncus truttae
    using TEM and NanoSIMS. <i>Science of The Total Environment</i>. 2023;887. doi:<a
    href="https://doi.org/10.1016/j.scitotenv.2023.164010">10.1016/j.scitotenv.2023.164010</a>
  apa: Filipović Marijić, V., Subirana, M. A., Schaumlöffel, D., Barišić, J., Gontier,
    E., Krasnici, N., … Erk, M. (2023). First insight in element localisation in different
    body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS.
    <i>Science of The Total Environment</i>. Elsevier. <a href="https://doi.org/10.1016/j.scitotenv.2023.164010">https://doi.org/10.1016/j.scitotenv.2023.164010</a>
  chicago: Filipović Marijić, Vlatka, Maria Angels Subirana, Dirk Schaumlöffel, Josip
    Barišić, Etienne Gontier, Nesrete Krasnici, Tatjana Mijošek, Jesús S. Hernández-Orts,
    Tomáš Scholz, and Marijana Erk. “First Insight in Element Localisation in Different
    Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and NanoSIMS.”
    <i>Science of The Total Environment</i>. Elsevier, 2023. <a href="https://doi.org/10.1016/j.scitotenv.2023.164010">https://doi.org/10.1016/j.scitotenv.2023.164010</a>.
  ieee: V. Filipović Marijić <i>et al.</i>, “First insight in element localisation
    in different body parts of the acanthocephalan Dentitruncus truttae using TEM
    and NanoSIMS,” <i>Science of The Total Environment</i>, vol. 887. Elsevier, 2023.
  ista: Filipović Marijić V, Subirana MA, Schaumlöffel D, Barišić J, Gontier E, Krasnici
    N, Mijošek T, Hernández-Orts JS, Scholz T, Erk M. 2023. First insight in element
    localisation in different body parts of the acanthocephalan Dentitruncus truttae
    using TEM and NanoSIMS. Science of The Total Environment. 887, 164010.
  mla: Filipović Marijić, Vlatka, et al. “First Insight in Element Localisation in
    Different Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and
    NanoSIMS.” <i>Science of The Total Environment</i>, vol. 887, 164010, Elsevier,
    2023, doi:<a href="https://doi.org/10.1016/j.scitotenv.2023.164010">10.1016/j.scitotenv.2023.164010</a>.
  short: V. Filipović Marijić, M.A. Subirana, D. Schaumlöffel, J. Barišić, E. Gontier,
    N. Krasnici, T. Mijošek, J.S. Hernández-Orts, T. Scholz, M. Erk, Science of The
    Total Environment 887 (2023).
date_created: 2024-01-10T10:43:08Z
date_published: 2023-08-20T00:00:00Z
date_updated: 2025-04-24T11:35:33Z
day: '20'
department:
- _id: LifeSc
doi: 10.1016/j.scitotenv.2023.164010
external_id:
  isi:
  - '001002645100001'
  pmid:
  - '37169189'
intvolume: '       887'
isi: 1
keyword:
- Pollution
- Waste Management and Disposal
- Environmental Chemistry
- Environmental Engineering
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://hal.science/hal-04296657/document
month: '08'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Science of The Total Environment
publication_identifier:
  issn:
  - 0048-9697
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: First insight in element localisation in different body parts of the acanthocephalan
  Dentitruncus truttae using TEM and NanoSIMS
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 887
year: '2023'
...