Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers Hansen, Andi H Hippenmeyer, Simon ; https://orcid.org/0000-0003-2279-1061 ddc:570 Mosaic analysis with double markers (MADM) technology enables the sparse labeling of genetically defined neurons. We present a protocol for time-lapse imaging of cortical projection neuron migration in mice using MADM. We describe steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled brain tissue. While this protocol is compatible with other single-cell labeling methods, the MADM approach provides a genetic platform for the functional assessment of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous effects. For complete details on the use and execution of this protocol, please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and Hippenmeyer (2021).3 Elsevier 2024 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_dcae04bc https://research-explorer.ista.ac.at/record/14794 https://research-explorer.ista.ac.at/download/14794/17264 Hansen AH, Hippenmeyer S. Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers. <i>STAR Protocols</i>. 2024;5(1). doi:<a href="https://doi.org/10.1016/j.xpro.2023.102795">10.1016/j.xpro.2023.102795</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2023.102795 info:eu-repo/semantics/altIdentifier/e-issn/2666-1667 info:eu-repo/semantics/altIdentifier/pmid/38165800 info:eu-repo/semantics/openAccess