article published yes Andi H Hansen author 38853E16-F248-11E8-B48F-1D18A9856A87 Simon Hippenmeyer author 37B36620-F248-11E8-B48F-1D18A9856A870000-0003-2279-1061 SiHi department Molecular mechanisms of radial neuronal migration project Mosaic analysis with double markers (MADM) technology enables the sparse labeling of genetically defined neurons. We present a protocol for time-lapse imaging of cortical projection neuron migration in mice using MADM. We describe steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled brain tissue. While this protocol is compatible with other single-cell labeling methods, the MADM approach provides a genetic platform for the functional assessment of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous effects. For complete details on the use and execution of this protocol, please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and Hippenmeyer (2021).3 https://research-explorer.ista.ac.at/download/14794/17264/2024_STARProtoc_Hansen.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ Elsevier2024 eng 2666-1667 3816580010.1016/j.xpro.2023.102795 51 http://github.com/hippenmeyerlab Hansen, Andi H., and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical Projection Neuron Migration in Mice Using Mosaic Analysis with Double Markers.” <i>STAR Protocols</i>, vol. 5, no. 1, 102795, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xpro.2023.102795">10.1016/j.xpro.2023.102795</a>. A.H. Hansen, S. Hippenmeyer, STAR Protocols 5 (2024). A. H. Hansen and S. Hippenmeyer, “Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers,” <i>STAR Protocols</i>, vol. 5, no. 1. Elsevier, 2024. Hansen, Andi H, and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical Projection Neuron Migration in Mice Using Mosaic Analysis with Double Markers.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2023.102795">https://doi.org/10.1016/j.xpro.2023.102795</a>. Hansen AH, Hippenmeyer S. Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers. <i>STAR Protocols</i>. 2024;5(1). doi:<a href="https://doi.org/10.1016/j.xpro.2023.102795">10.1016/j.xpro.2023.102795</a> Hansen, A. H., &#38; Hippenmeyer, S. (2024). Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers. <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2023.102795">https://doi.org/10.1016/j.xpro.2023.102795</a> Hansen AH, Hippenmeyer S. 2024. Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers. STAR Protocols. 5(1), 102795. 147942024-01-14T23:00:56Z2025-04-15T07:32:40Z