---
_id: '14794'
abstract:
- lang: eng
  text: "Mosaic analysis with double markers (MADM) technology enables the sparse
    labeling of genetically defined neurons. We present a protocol for time-lapse
    imaging of cortical projection neuron migration in mice using MADM. We describe
    steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled
    brain tissue. While this protocol is compatible with other single-cell labeling
    methods, the MADM approach provides a genetic platform for the functional assessment
    of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous
    effects.\r\n\r\nFor complete details on the use and execution of this protocol,
    please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and
    Hippenmeyer (2021).3"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Florian Pauler for discussion and his expert technical support.
  This research was supported by the Scientific Service Units (SSU) at IST Austria
  through resources provided by the Imaging and Optics Facility (IOF) and Preclinical
  Facility (PCF). A.H.H. was a recipient of a DOC Fellowship (24812) of the Austrian
  Academy of Sciences.
article_number: '102795'
article_processing_charge: Yes
article_type: review
author:
- first_name: Andi H
  full_name: Hansen, Andi H
  id: 38853E16-F248-11E8-B48F-1D18A9856A87
  last_name: Hansen
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Hansen AH, Hippenmeyer S. Time-lapse imaging of cortical projection neuron
    migration in mice using mosaic analysis with double markers. <i>STAR Protocols</i>.
    2024;5(1). doi:<a href="https://doi.org/10.1016/j.xpro.2023.102795">10.1016/j.xpro.2023.102795</a>
  apa: Hansen, A. H., &#38; Hippenmeyer, S. (2024). Time-lapse imaging of cortical
    projection neuron migration in mice using mosaic analysis with double markers.
    <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2023.102795">https://doi.org/10.1016/j.xpro.2023.102795</a>
  chicago: Hansen, Andi H, and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical
    Projection Neuron Migration in Mice Using Mosaic Analysis with Double Markers.”
    <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2023.102795">https://doi.org/10.1016/j.xpro.2023.102795</a>.
  ieee: A. H. Hansen and S. Hippenmeyer, “Time-lapse imaging of cortical projection
    neuron migration in mice using mosaic analysis with double markers,” <i>STAR Protocols</i>,
    vol. 5, no. 1. Elsevier, 2024.
  ista: Hansen AH, Hippenmeyer S. 2024. Time-lapse imaging of cortical projection
    neuron migration in mice using mosaic analysis with double markers. STAR Protocols.
    5(1), 102795.
  mla: Hansen, Andi H., and Simon Hippenmeyer. “Time-Lapse Imaging of Cortical Projection
    Neuron Migration in Mice Using Mosaic Analysis with Double Markers.” <i>STAR Protocols</i>,
    vol. 5, no. 1, 102795, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xpro.2023.102795">10.1016/j.xpro.2023.102795</a>.
  short: A.H. Hansen, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2024-01-14T23:00:56Z
date_published: 2024-03-15T00:00:00Z
date_updated: 2025-04-15T07:32:40Z
day: '15'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2023.102795
external_id:
  pmid:
  - '38165800'
file:
- access_level: open_access
  checksum: 4644d537451c5c114a9d7c7829b65bba
  content_type: application/pdf
  creator: dernst
  date_created: 2024-07-16T12:04:46Z
  date_updated: 2024-07-16T12:04:46Z
  file_id: '17264'
  file_name: 2024_STARProtoc_Hansen.pdf
  file_size: 3758943
  relation: main_file
  success: 1
file_date_updated: 2024-07-16T12:04:46Z
has_accepted_license: '1'
intvolume: '         5'
issue: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2625A13E-B435-11E9-9278-68D0E5697425
  grant_number: '24812'
  name: Molecular mechanisms of radial neuronal migration
publication: STAR Protocols
publication_identifier:
  eissn:
  - 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - relation: software
    url: http://github.com/hippenmeyerlab
scopus_import: '1'
status: public
title: Time-lapse imaging of cortical projection neuron migration in mice using mosaic
  analysis with double markers
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...