---
_id: '14834'
abstract:
- lang: eng
  text: Bacteria divide by binary fission. The protein machine responsible for this
    process is the divisome, a transient assembly of more than 30 proteins in and
    on the surface of the cytoplasmic membrane. Together, they constrict the cell
    envelope and remodel the peptidoglycan layer to eventually split the cell into
    two. For Escherichia coli, most molecular players involved in this process have
    probably been identified, but obtaining the quantitative information needed for
    a mechanistic understanding can often not be achieved from experiments in vivo
    alone. Since the discovery of the Z-ring more than 30 years ago, in vitro reconstitution
    experiments have been crucial to shed light on molecular processes normally hidden
    in the complex environment of the living cell. In this review, we summarize how
    rebuilding the divisome from purified components – or at least parts of it - have
    been instrumental to obtain the detailed mechanistic understanding of the bacterial
    cell division machinery that we have today.
acknowledgement: We acknowledge members of the Loose laboratory at ISTA for helpful
  discussions—in particular M. Kojic for his insightful comments. This work was supported
  by the Austrian Science Fund (FWF P34607) to M.L.
article_number: '151380'
article_processing_charge: Yes
article_type: review
author:
- first_name: Philipp
  full_name: Radler, Philipp
  id: 40136C2A-F248-11E8-B48F-1D18A9856A87
  last_name: Radler
  orcid: '0000-0001-9198-2182 '
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: 'Radler P, Loose M. A dynamic duo: Understanding the roles of FtsZ and FtsA
    for Escherichia coli cell division through in vitro approaches. <i>European Journal
    of Cell Biology</i>. 2024;103(1). doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>'
  apa: 'Radler, P., &#38; Loose, M. (2024). A dynamic duo: Understanding the roles
    of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches.
    <i>European Journal of Cell Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>'
  chicago: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.ejcb.2023.151380">https://doi.org/10.1016/j.ejcb.2023.151380</a>.'
  ieee: 'P. Radler and M. Loose, “A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches,” <i>European
    Journal of Cell Biology</i>, vol. 103, no. 1. Elsevier, 2024.'
  ista: 'Radler P, Loose M. 2024. A dynamic duo: Understanding the roles of FtsZ and
    FtsA for Escherichia coli cell division through in vitro approaches. European
    Journal of Cell Biology. 103(1), 151380.'
  mla: 'Radler, Philipp, and Martin Loose. “A Dynamic Duo: Understanding the Roles
    of FtsZ and FtsA for Escherichia Coli Cell Division through in Vitro Approaches.”
    <i>European Journal of Cell Biology</i>, vol. 103, no. 1, 151380, Elsevier, 2024,
    doi:<a href="https://doi.org/10.1016/j.ejcb.2023.151380">10.1016/j.ejcb.2023.151380</a>.'
  short: P. Radler, M. Loose, European Journal of Cell Biology 103 (2024).
corr_author: '1'
date_created: 2024-01-18T08:16:43Z
date_published: 2024-03-01T00:00:00Z
date_updated: 2025-09-04T11:45:31Z
day: '01'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1016/j.ejcb.2023.151380
external_id:
  isi:
  - '001166216800001'
  pmid:
  - '38218128'
file:
- access_level: open_access
  checksum: 5d170abbc87585205c010657e4552360
  content_type: application/pdf
  creator: dernst
  date_created: 2024-07-16T12:07:20Z
  date_updated: 2024-07-16T12:07:20Z
  file_id: '17265'
  file_name: 2024_EJCB_Radler.pdf
  file_size: 9995304
  relation: main_file
  success: 1
file_date_updated: 2024-07-16T12:07:20Z
has_accepted_license: '1'
intvolume: '       103'
isi: 1
issue: '1'
keyword:
- Cell Biology
- General Medicine
- Histology
- Pathology and Forensic Medicine
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: fc38323b-9c52-11eb-aca3-ff8afb4a011d
  grant_number: P34607
  name: In vitro reconstitution of bacterial cell division
publication: European Journal of Cell Biology
publication_identifier:
  issn:
  - 0171-9335
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli
  cell division through in vitro approaches'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 103
year: '2024'
...