--- res: bibo_abstract: - To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with a preserved prolif eration capacity. The root pericycle represents a unique tissue with conditional meristematic activity, and its tight control determines initiation of lateral organs. Here we show that the meristematic activity of the pericycle is constrained by the interaction with the adjacent endodermis. Release of these restraints by elimination of endo dermal cells by single-cell ablation triggers the pericycle to re-enter the cell cycle. We found that endodermis removal substitutes for the phytohormone auxin-dependent initiation of the pericycle meristematic activity. However, auxin is indispensable to steer the cell division plane orientation of new organ-defining divisions. We propose a dual, spatiotemporally distinct role for auxin during lateral root initiation. In the endodermis, auxin releases constraints arising from cell-to-cell interactions that compromise the pericycle meristematic activity, whereas, in the pericycle, auxin defines the orientation of the cell division plane to initiate lateral roots.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Peter foaf_name: Marhavy, Peter foaf_surname: Marhavy foaf_workInfoHomepage: http://www.librecat.org/personId=3F45B078-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-5227-5741 - foaf_Person: foaf_givenName: Juan C foaf_name: Montesinos López, Juan C foaf_surname: Montesinos López foaf_workInfoHomepage: http://www.librecat.org/personId=310A8E3E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9179-6099 - foaf_Person: foaf_givenName: Anas foaf_name: Abuzeineh, Anas foaf_surname: Abuzeineh - foaf_Person: foaf_givenName: Daniël foaf_name: Van Damme, Daniël foaf_surname: Van Damme - foaf_Person: foaf_givenName: Joop foaf_name: Vermeer, Joop foaf_surname: Vermeer - foaf_Person: foaf_givenName: Jérôme foaf_name: Duclercq, Jérôme foaf_surname: Duclercq - foaf_Person: foaf_givenName: Hana foaf_name: Rakusova, Hana foaf_surname: Rakusova - foaf_Person: foaf_givenName: Petra foaf_name: Marhavá, Petra foaf_surname: Marhavá foaf_workInfoHomepage: http://www.librecat.org/personId=44E59624-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Jirí foaf_name: Friml, Jirí foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Niko foaf_name: Geldner, Niko foaf_surname: Geldner - foaf_Person: foaf_givenName: Eva foaf_name: Benková, Eva foaf_surname: Benková foaf_workInfoHomepage: http://www.librecat.org/personId=38F4F166-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8510-9739 bibo_doi: 10.1101/gad.276964.115 bibo_issue: '4' bibo_volume: 30 dct_date: 2016^xs_gYear dct_language: eng dct_publisher: Cold Spring Harbor Laboratory Press@ dct_title: Targeted cell elimination reveals an auxin-guided biphasic mode of lateral root initiation@ fabio_hasPubmedId: ' 26883363' ...