Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores

article Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores published yes Julia Datler author 3B12E2E6-F248-11E8-B48F-1D18A9856A870000-0002-3616-8580 Jesse Hansen author 1063c618-6f9b-11ec-9123-f912fccded630000-0001-7967-2085 Andreas Thader author 3A18A7B8-F248-11E8-B48F-1D18A9856A87 Alois Schlögl author 45BF87EE-F248-11E8-B48F-1D18A9856A870000-0002-5621-8100 Lukas W Bauer author 0c894dcf-897b-11ed-a09c-8186353224b0 Victor-Valentin Hodirnau author 3661B498-F248-11E8-B48F-1D18A9856A870000-0003-3904-947X Florian KM Schur author 48AD8942-F248-11E8-B48F-1D18A9856A870000-0003-4790-8078 FlSc department ScienComp department EM-Fac department Structural conservation and diversity in retroviral capsid project Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses. https://research-explorer.ista.ac.at/download/14979/17307/2024_NatureStrucBio_Datler.pdf application/pdfno Springer Nature2024 eng Molecular BiologyStructural Biology Nature Structural & Molecular Biology 1545-9993 1545-9985 38316877 00115814460000210.1038/s41594-023-01201-6 311114-1123 https://research-explorer.ista.ac.at/record/18766 https://ista.ac.at/en/news/down-to-the-core-of-poxviruses/ Datler, Julia, Jesse Hansen, Andreas Thader, Alois Schlögl, Lukas W Bauer, Victor-Valentin Hodirnau, and Florian KM Schur. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” Nature Structural & Molecular Biology. Springer Nature, 2024. <a href="https://doi.org/10.1038/s41594-023-01201-6">https://doi.org/10.1038/s41594-023-01201-6</a>. Datler, Julia, et al. “Multi-Modal Cryo-EM Reveals Trimers of Protein A10 to Form the Palisade Layer in Poxvirus Cores.” Nature Structural & Molecular Biology, vol. 31, Springer Nature, 2024, pp. 1114–23, doi:<a href="https://doi.org/10.1038/s41594-023-01201-6">10.1038/s41594-023-01201-6</a>. J. Datler et al., “Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores,” Nature Structural & Molecular Biology, vol. 31. Springer Nature, pp. 1114–1123, 2024. Datler J, Hansen J, Thader A, Schlögl A, Bauer LW, Hodirnau V-V, Schur FK. 2024. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. Nature Structural & Molecular Biology. 31, 1114–1123. J. Datler, J. Hansen, A. Thader, A. Schlögl, L.W. Bauer, V.-V. Hodirnau, F.K. Schur, Nature Structural & Molecular Biology 31 (2024) 1114–1123. Datler, J., Hansen, J., Thader, A., Schlögl, A., Bauer, L. W., Hodirnau, V.-V., & Schur, F. K. (2024). Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. Nature Structural & Molecular Biology. Springer Nature. <a href="https://doi.org/10.1038/s41594-023-01201-6">https://doi.org/10.1038/s41594-023-01201-6</a> Datler J, Hansen J, Thader A, et al. Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. Nature Structural & Molecular Biology. 2024;31:1114-1123. doi:<a href="https://doi.org/10.1038/s41594-023-01201-6">10.1038/s41594-023-01201-6</a> 149792024-02-12T09:59:45Z2026-04-07T12:59:44Z