{"title":"VRK-1 extends life span by activation of AMPK via phosphorylation","file":[{"success":1,"date_updated":"2024-03-04T09:46:41Z","relation":"main_file","content_type":"application/pdf","date_created":"2024-03-04T09:46:41Z","file_size":1864415,"creator":"dernst","file_name":"2020_ScienceAdvances_Park.pdf","access_level":"open_access","file_id":"15058","checksum":"a37157cd0de709dce5fe03f4a31cd0b6"}],"license":"https://creativecommons.org/licenses/by-nc/4.0/","publisher":"American Association for the Advancement of Science","month":"07","date_published":"2020-07-01T00:00:00Z","year":"2020","article_processing_charge":"No","publication_status":"published","intvolume":"         6","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"eissn":["2375-2548"]},"quality_controlled":"1","_id":"15057","publication":"Science Advances","doi":"10.1126/sciadv.aaw7824","type":"journal_article","day":"01","has_accepted_license":"1","status":"public","date_updated":"2024-03-04T09:52:09Z","ddc":["570"],"oa_version":"Published Version","department":[{"_id":"MaDe"}],"article_number":"aaw7824","acknowledgement":"This research was supported by grants NRF-2019R1A3B2067745 and NRF-2017R1A5A1015366 funded by the Korean Government (MSIT) through the National Research Foundation (NRF) of Korea to S.-J.V.L. and by grant Basic Science Research Program (No. 2019R1A2C2009440) funded by the Korean Government (MSIT) through the NRF of Korea to K.-T.K. ","author":[{"last_name":"Park","first_name":"Sangsoon","full_name":"Park, Sangsoon"},{"last_name":"Artan","id":"C407B586-6052-11E9-B3AE-7006E6697425","orcid":"0000-0001-8945-6992","full_name":"Artan, Murat","first_name":"Murat"},{"first_name":"Seung Hyun","full_name":"Han, Seung Hyun","last_name":"Han"},{"last_name":"Park","full_name":"Park, Hae-Eun H.","first_name":"Hae-Eun H."},{"full_name":"Jung, Yoonji","first_name":"Yoonji","last_name":"Jung"},{"first_name":"Ara B.","full_name":"Hwang, Ara B.","last_name":"Hwang"},{"last_name":"Shin","first_name":"Won Sik","full_name":"Shin, Won Sik"},{"first_name":"Kyong-Tai","full_name":"Kim, Kyong-Tai","last_name":"Kim"},{"full_name":"Lee, Seung-Jae V.","first_name":"Seung-Jae V.","last_name":"Lee"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","short":"CC BY-NC (4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"language":[{"iso":"eng"}],"issue":"27","file_date_updated":"2024-03-04T09:46:41Z","article_type":"original","oa":1,"abstract":[{"lang":"eng","text":"Vaccinia virus–related kinase (VRK) is an evolutionarily conserved nuclear protein kinase. VRK-1, the single Caenorhabditis elegans VRK ortholog, functions in cell division and germline proliferation. However, the role of VRK-1 in postmitotic cells and adult life span remains unknown. Here, we show that VRK-1 increases organismal longevity by activating the cellular energy sensor, AMP-activated protein kinase (AMPK), via direct phosphorylation. We found that overexpression of vrk-1 in the soma of adult C. elegans increased life span and, conversely, inhibition of vrk-1 decreased life span. In addition, vrk-1 was required for longevity conferred by mutations that inhibit C. elegans mitochondrial respiration, which requires AMPK. VRK-1 directly phosphorylated and up-regulated AMPK in both C. elegans and cultured human cells. Thus, our data show that the somatic nuclear kinase, VRK-1, promotes longevity through AMPK activation, and this function appears to be conserved between C. elegans and humans."}],"citation":{"short":"S. Park, M. Artan, S.H. Han, H.-E.H. Park, Y. Jung, A.B. Hwang, W.S. Shin, K.-T. Kim, S.-J.V. Lee, Science Advances 6 (2020).","chicago":"Park, Sangsoon, Murat Artan, Seung Hyun Han, Hae-Eun H. Park, Yoonji Jung, Ara B. Hwang, Won Sik Shin, Kyong-Tai Kim, and Seung-Jae V. Lee. “VRK-1 Extends Life Span by Activation of AMPK via Phosphorylation.” <i>Science Advances</i>. American Association for the Advancement of Science, 2020. <a href=\"https://doi.org/10.1126/sciadv.aaw7824\">https://doi.org/10.1126/sciadv.aaw7824</a>.","ieee":"S. Park <i>et al.</i>, “VRK-1 extends life span by activation of AMPK via phosphorylation,” <i>Science Advances</i>, vol. 6, no. 27. American Association for the Advancement of Science, 2020.","ama":"Park S, Artan M, Han SH, et al. VRK-1 extends life span by activation of AMPK via phosphorylation. <i>Science Advances</i>. 2020;6(27). doi:<a href=\"https://doi.org/10.1126/sciadv.aaw7824\">10.1126/sciadv.aaw7824</a>","ista":"Park S, Artan M, Han SH, Park H-EH, Jung Y, Hwang AB, Shin WS, Kim K-T, Lee S-JV. 2020. VRK-1 extends life span by activation of AMPK via phosphorylation. Science Advances. 6(27), aaw7824.","mla":"Park, Sangsoon, et al. “VRK-1 Extends Life Span by Activation of AMPK via Phosphorylation.” <i>Science Advances</i>, vol. 6, no. 27, aaw7824, American Association for the Advancement of Science, 2020, doi:<a href=\"https://doi.org/10.1126/sciadv.aaw7824\">10.1126/sciadv.aaw7824</a>.","apa":"Park, S., Artan, M., Han, S. H., Park, H.-E. H., Jung, Y., Hwang, A. B., … Lee, S.-J. V. (2020). VRK-1 extends life span by activation of AMPK via phosphorylation. <i>Science Advances</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/sciadv.aaw7824\">https://doi.org/10.1126/sciadv.aaw7824</a>"},"volume":6,"date_created":"2024-03-04T09:41:57Z"}