---
res:
  bibo_abstract:
  - "A mesophilic methanogenic culture, designated JL01, was isolated from Holocene
    permafrost in the Russian Arctic [1]. After long-term extensive cultivation at
    15°C it turned out to be a tied binary culture of archaeal (JL01) and bacterial
    (Sphaerochaeta associata GLS2) strains.\r\nStrain JL01 was a strict anaerobe and
    grew on methanol, acetate and methylamines as energy and carbon sources. Cells
    were irregular coccoid, non-motile, non-spore-forming, and Gram-stainpositive.
    Optimum conditions for growth were 24-28 oC, pH 6.8–7.3 and 0.075-0.1 M NaCl.\r\nPhylogenetic
    tree reconstructions based on 16S rRNA and concatenated alignment of broadly\r\nconserved
    protein-coding genes revealed its close relation to Methanosarcina mazei S-6\r\nT
    (similarity 99.5%). The comparison of whole genomic sequences (ANI) of the isolate
    and the type strain of M.mazei was 98.5%, which is higher than the values recommended
    for new species. Thus strain JL01 (=VKM B-2370=JCM 31898) represents the first
    M. mazei isolated from permanently subzero Arcticsediments. The long-term co-cultivation
    of JL01 with S. associata GLS2T showed the methane production without any additional
    carbon and energy sources. Genome analysis of S. associata GLS2T revealed putative
    genes involved in methanochondroithin catabolism.@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Viktoriia
      foaf_name: Oshurkova, Viktoriia
      foaf_surname: Oshurkova
  - foaf_Person:
      foaf_givenName: Olga
      foaf_name: Troshina, Olga
      foaf_surname: Troshina
  - foaf_Person:
      foaf_givenName: Vladimir
      foaf_name: Trubitsyn, Vladimir
      foaf_surname: Trubitsyn
  - foaf_Person:
      foaf_givenName: Yana
      foaf_name: Ryzhmanova, Yana
      foaf_surname: Ryzhmanova
  - foaf_Person:
      foaf_givenName: Olga
      foaf_name: Bochkareva, Olga
      foaf_surname: Bochkareva
      foaf_workInfoHomepage: http://www.librecat.org/personId=C4558D3C-6102-11E9-A62E-F418E6697425
    orcid: 0000-0003-1006-6639
  - foaf_Person:
      foaf_givenName: Viktoria
      foaf_name: Shcherbakova, Viktoria
      foaf_surname: Shcherbakova
  bibo_doi: 10.3390/ecm2020-07116
  dct_date: 2020^xs_gYear
  dct_language: eng
  dct_publisher: MDPI@
  dct_title: Characterization of methanosarcina mazei JL01 isolated from holocene
    arctic permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
    associata GLS2T@
...