---
_id: '15071'
abstract:
- lang: eng
  text: "A mesophilic methanogenic culture, designated JL01, was isolated from Holocene
    permafrost in the Russian Arctic [1]. After long-term extensive cultivation at
    15°C it turned out to be a tied binary culture of archaeal (JL01) and bacterial
    (Sphaerochaeta associata GLS2) strains.\r\nStrain JL01 was a strict anaerobe and
    grew on methanol, acetate and methylamines as energy and carbon sources. Cells
    were irregular coccoid, non-motile, non-spore-forming, and Gram-stainpositive.
    Optimum conditions for growth were 24-28 oC, pH 6.8–7.3 and 0.075-0.1 M NaCl.\r\nPhylogenetic
    tree reconstructions based on 16S rRNA and concatenated alignment of broadly\r\nconserved
    protein-coding genes revealed its close relation to Methanosarcina mazei S-6\r\nT
    (similarity 99.5%). The comparison of whole genomic sequences (ANI) of the isolate
    and the type strain of M.mazei was 98.5%, which is higher than the values recommended
    for new species. Thus strain JL01 (=VKM B-2370=JCM 31898) represents the first
    M. mazei isolated from permanently subzero Arcticsediments. The long-term co-cultivation
    of JL01 with S. associata GLS2T showed the methane production without any additional
    carbon and energy sources. Genome analysis of S. associata GLS2T revealed putative
    genes involved in methanochondroithin catabolism."
acknowledgement: "The work was supported by of Russian Foundation of Basic Research:
  grant № 19-04-00831 for Viktoria Shcherbakova and Olga Troshina, grant № 18-34-00334
  for Viktoriia Oshurkova and Vladimir Trubitsyn. \r\nWe thank Dr Natalia Suzina (IBPM
  RAS, Federal Research Center Pushchino Center for\r\nBiological Research RAS) for
  the help with the microscopic studies, respectively; Dr. Margarita Meyer (Division
  of Genetics, Department of Medicine, BWH and HMS, USA) and Dr Fedor Kondrashov (IST,
  Austria) for their help in obtaining the genomic sequence of strain JL01. "
article_processing_charge: Yes
author:
- first_name: Viktoriia
  full_name: Oshurkova, Viktoriia
  last_name: Oshurkova
- first_name: Olga
  full_name: Troshina, Olga
  last_name: Troshina
- first_name: Vladimir
  full_name: Trubitsyn, Vladimir
  last_name: Trubitsyn
- first_name: Yana
  full_name: Ryzhmanova, Yana
  last_name: Ryzhmanova
- first_name: Olga
  full_name: Bochkareva, Olga
  id: C4558D3C-6102-11E9-A62E-F418E6697425
  last_name: Bochkareva
  orcid: 0000-0003-1006-6639
- first_name: Viktoria
  full_name: Shcherbakova, Viktoria
  last_name: Shcherbakova
citation:
  ama: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
    V. Characterization of methanosarcina mazei JL01 isolated from holocene arctic
    permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
    associata GLS2T. In: <i>Proceedings of 1st International Electronic Conference
    on Microbiology</i>. MDPI; 2020. doi:<a href="https://doi.org/10.3390/ecm2020-07116">10.3390/ecm2020-07116</a>'
  apa: 'Oshurkova, V., Troshina, O., Trubitsyn, V., Ryzhmanova, Y., Bochkareva, O.,
    &#38; Shcherbakova, V. (2020). Characterization of methanosarcina mazei JL01 isolated
    from holocene arctic permafrost and study of the archaeon cooperation with bacterium
    Sphaerochaeta associata GLS2T. In <i>Proceedings of 1st International Electronic
    Conference on Microbiology</i>. Virtual: MDPI. <a href="https://doi.org/10.3390/ecm2020-07116">https://doi.org/10.3390/ecm2020-07116</a>'
  chicago: Oshurkova, Viktoriia, Olga Troshina, Vladimir Trubitsyn, Yana Ryzhmanova,
    Olga Bochkareva, and Viktoria Shcherbakova. “Characterization of Methanosarcina
    Mazei JL01 Isolated from Holocene Arctic Permafrost and Study of the Archaeon
    Cooperation with Bacterium Sphaerochaeta Associata GLS2T.” In <i>Proceedings of
    1st International Electronic Conference on Microbiology</i>. MDPI, 2020. <a href="https://doi.org/10.3390/ecm2020-07116">https://doi.org/10.3390/ecm2020-07116</a>.
  ieee: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, and
    V. Shcherbakova, “Characterization of methanosarcina mazei JL01 isolated from
    holocene arctic permafrost and study of the archaeon cooperation with bacterium
    Sphaerochaeta associata GLS2T,” in <i>Proceedings of 1st International Electronic
    Conference on Microbiology</i>, Virtual, 2020.
  ista: 'Oshurkova V, Troshina O, Trubitsyn V, Ryzhmanova Y, Bochkareva O, Shcherbakova
    V. 2020. Characterization of methanosarcina mazei JL01 isolated from holocene
    arctic permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta
    associata GLS2T. Proceedings of 1st International Electronic Conference on Microbiology.
    ECM: Electronic Conference on Microbiology.'
  mla: Oshurkova, Viktoriia, et al. “Characterization of Methanosarcina Mazei JL01
    Isolated from Holocene Arctic Permafrost and Study of the Archaeon Cooperation
    with Bacterium Sphaerochaeta Associata GLS2T.” <i>Proceedings of 1st International
    Electronic Conference on Microbiology</i>, MDPI, 2020, doi:<a href="https://doi.org/10.3390/ecm2020-07116">10.3390/ecm2020-07116</a>.
  short: V. Oshurkova, O. Troshina, V. Trubitsyn, Y. Ryzhmanova, O. Bochkareva, V.
    Shcherbakova, in:, Proceedings of 1st International Electronic Conference on Microbiology,
    MDPI, 2020.
conference:
  end_date: 2020-11-30
  location: Virtual
  name: 'ECM: Electronic Conference on Microbiology'
  start_date: 2020-11-02
date_created: 2024-03-04T11:41:31Z
date_published: 2020-11-02T00:00:00Z
date_updated: 2024-03-20T08:06:22Z
day: '02'
ddc:
- '570'
department:
- _id: FyKo
doi: 10.3390/ecm2020-07116
file:
- access_level: open_access
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  creator: dernst
  date_created: 2024-03-20T08:05:46Z
  date_updated: 2024-03-20T08:05:46Z
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  file_name: 2020_ECM_Oshurkova.pdf
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file_date_updated: 2024-03-20T08:05:46Z
has_accepted_license: '1'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Proceedings of 1st International Electronic Conference on Microbiology
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: Characterization of methanosarcina mazei JL01 isolated from holocene arctic
  permafrost and study of the archaeon cooperation with bacterium Sphaerochaeta associata
  GLS2T
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: conference
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...