{"year":"2023","title":"Structural snapshots of R-loop formation by a type I-C CRISPR Cascade","_id":"15129","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":83,"scopus_import":"1","page":"746-758.e5","oa":1,"extern":"1","publication":"Molecular Cell","author":[{"first_name":"Roisin E.","full_name":"O’Brien, Roisin E.","last_name":"O’Brien"},{"orcid":"0000-0003-0456-0753","last_name":"Bravo","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","full_name":"Bravo, Jack Peter Kelly","first_name":"Jack Peter Kelly"},{"last_name":"Ramos","full_name":"Ramos, Delisa","first_name":"Delisa"},{"last_name":"Hibshman","first_name":"Grace N.","full_name":"Hibshman, Grace N."},{"first_name":"Jacquelyn T.","full_name":"Wright, Jacquelyn T.","last_name":"Wright"},{"last_name":"Taylor","first_name":"David W.","full_name":"Taylor, David W."}],"abstract":[{"lang":"eng","text":"Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type I-C Cascade at various stages of double-stranded (ds)DNA target capture, revealing mechanisms that underpin PAM recognition and Cascade allosteric activation. We uncover an interesting mechanism of non-target strand (NTS) DNA stabilization via stacking interactions with the “belly” subunits, securing the NTS in place. This “molecular seatbelt” mechanism facilitates efficient R-loop formation and prevents dsDNA reannealing. Additionally, we provide structural insights into how two anti-CRISPR (Acr) proteins utilize distinct strategies to achieve a shared mechanism of type I-C Cascade inhibition by blocking PAM scanning. These observations form a structural basis for directional R-loop formation and reveal how different Acr proteins have converged upon common molecular mechanisms to efficiently shut down CRISPR immunity."}],"date_published":"2023-03-02T00:00:00Z","quality_controlled":"1","publisher":"Elsevier","issue":"5","article_type":"original","pmid":1,"type":"journal_article","language":[{"iso":"eng"}],"month":"03","intvolume":"        83","main_file_link":[{"url":"https://doi.org/10.1016/j.molcel.2023.01.024","open_access":"1"}],"citation":{"short":"R.E. O’Brien, J.P.K. Bravo, D. Ramos, G.N. Hibshman, J.T. Wright, D.W. Taylor, Molecular Cell 83 (2023) 746–758.e5.","chicago":"O’Brien, Roisin E., Jack Peter Kelly Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, and David W. Taylor. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>.","ieee":"R. E. O’Brien, J. P. K. Bravo, D. Ramos, G. N. Hibshman, J. T. Wright, and D. W. Taylor, “Structural snapshots of R-loop formation by a type I-C CRISPR Cascade,” <i>Molecular Cell</i>, vol. 83, no. 5. Elsevier, p. 746–758.e5, 2023.","ista":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. 2023. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell. 83(5), 746–758.e5.","apa":"O’Brien, R. E., Bravo, J. P. K., Ramos, D., Hibshman, G. N., Wright, J. T., &#38; Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>","mla":"O’Brien, Roisin E., et al. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>, vol. 83, no. 5, Elsevier, 2023, p. 746–758.e5, doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>.","ama":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. 2023;83(5):746-758.e5. doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>"},"status":"public","day":"02","date_updated":"2024-06-04T06:33:54Z","publication_status":"published","article_processing_charge":"Yes (in subscription journal)","oa_version":"Published Version","date_created":"2024-03-20T10:40:56Z","doi":"10.1016/j.molcel.2023.01.024","external_id":{"pmid":["36805026"]},"publication_identifier":{"issn":["1097-2765"]},"keyword":["Cell Biology","Molecular Biology"]}