{"date_published":"2023-03-02T00:00:00Z","day":"02","publication_status":"published","publication":"Molecular Cell","pmid":1,"author":[{"last_name":"O’Brien","full_name":"O’Brien, Roisin E.","first_name":"Roisin E."},{"first_name":"Jack Peter Kelly","last_name":"Bravo","full_name":"Bravo, Jack Peter Kelly","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","orcid":"0000-0003-0456-0753"},{"first_name":"Delisa","last_name":"Ramos","full_name":"Ramos, Delisa"},{"last_name":"Hibshman","full_name":"Hibshman, Grace N.","first_name":"Grace N."},{"first_name":"Jacquelyn T.","last_name":"Wright","full_name":"Wright, Jacquelyn T."},{"first_name":"David W.","full_name":"Taylor, David W.","last_name":"Taylor"}],"doi":"10.1016/j.molcel.2023.01.024","_id":"15129","volume":83,"external_id":{"pmid":["36805026"]},"intvolume":"        83","language":[{"iso":"eng"}],"quality_controlled":"1","oa":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.molcel.2023.01.024","open_access":"1"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"746-758.e5","date_created":"2024-03-20T10:40:56Z","citation":{"short":"R.E. O’Brien, J.P.K. Bravo, D. Ramos, G.N. Hibshman, J.T. Wright, D.W. Taylor, Molecular Cell 83 (2023) 746–758.e5.","ista":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. 2023. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell. 83(5), 746–758.e5.","ieee":"R. E. O’Brien, J. P. K. Bravo, D. Ramos, G. N. Hibshman, J. T. Wright, and D. W. Taylor, “Structural snapshots of R-loop formation by a type I-C CRISPR Cascade,” <i>Molecular Cell</i>, vol. 83, no. 5. Elsevier, p. 746–758.e5, 2023.","ama":"O’Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. 2023;83(5):746-758.e5. doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>","mla":"O’Brien, Roisin E., et al. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>, vol. 83, no. 5, Elsevier, 2023, p. 746–758.e5, doi:<a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">10.1016/j.molcel.2023.01.024</a>.","chicago":"O’Brien, Roisin E., Jack Peter Kelly Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, and David W. Taylor. “Structural Snapshots of R-Loop Formation by a Type I-C CRISPR Cascade.” <i>Molecular Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>.","apa":"O’Brien, R. E., Bravo, J. P. K., Ramos, D., Hibshman, G. N., Wright, J. T., &#38; Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. <i>Molecular Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molcel.2023.01.024\">https://doi.org/10.1016/j.molcel.2023.01.024</a>"},"type":"journal_article","date_updated":"2024-06-04T06:33:54Z","month":"03","title":"Structural snapshots of R-loop formation by a type I-C CRISPR Cascade","keyword":["Cell Biology","Molecular Biology"],"article_processing_charge":"Yes (in subscription journal)","scopus_import":"1","issue":"5","publisher":"Elsevier","abstract":[{"lang":"eng","text":"Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type I-C Cascade at various stages of double-stranded (ds)DNA target capture, revealing mechanisms that underpin PAM recognition and Cascade allosteric activation. We uncover an interesting mechanism of non-target strand (NTS) DNA stabilization via stacking interactions with the “belly” subunits, securing the NTS in place. This “molecular seatbelt” mechanism facilitates efficient R-loop formation and prevents dsDNA reannealing. Additionally, we provide structural insights into how two anti-CRISPR (Acr) proteins utilize distinct strategies to achieve a shared mechanism of type I-C Cascade inhibition by blocking PAM scanning. These observations form a structural basis for directional R-loop formation and reveal how different Acr proteins have converged upon common molecular mechanisms to efficiently shut down CRISPR immunity."}],"year":"2023","publication_identifier":{"issn":["1097-2765"]},"oa_version":"Published Version","article_type":"original","extern":"1","status":"public"}