---
res:
  bibo_abstract:
  - Clustered regularly interspaced short palindromic repeats - CRISPR-associated
    protein (CRISPR-Cas) systems are a critical component of the bacterial adaptive
    immune response. Since the discovery that they can be reengineered as programmable
    RNA-guided nucleases, there has been significant interest in using these systems
    to perform diverse and precise genetic manipulations. Here, we outline recent
    advances in the mechanistic understanding of CRISPR-Cas9, how these findings have
    been leveraged in the rational redesign of Cas9 variants with altered activities,
    and how these novel tools can be exploited for biotechnology and therapeutics.
    We also discuss the potential of the ubiquitous, yet often-overlooked, multisubunit
    CRISPR effector complexes for large-scale genomic deletions. Furthermore, we highlight
    how future structural studies will bolster these technologies.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jack Peter Kelly
      foaf_name: Bravo, Jack Peter Kelly
      foaf_surname: Bravo
      foaf_workInfoHomepage: http://www.librecat.org/personId=96aecfa5-8931-11ee-af30-aa6a5d6eee0e
    orcid: 0000-0003-0456-0753
  - foaf_Person:
      foaf_givenName: Grace N
      foaf_name: Hibshman, Grace N
      foaf_surname: Hibshman
  - foaf_Person:
      foaf_givenName: David W
      foaf_name: Taylor, David W
      foaf_surname: Taylor
  bibo_doi: 10.1016/j.copbio.2022.102839
  bibo_volume: 78
  dct_date: 2022^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0958-1669
  dct_language: eng
  dct_publisher: Elsevier@
  dct_subject:
  - Biomedical Engineering
  - Bioengineering
  - Biotechnology
  dct_title: Constructing next-generation CRISPR–Cas tools from structural blueprints@
  fabio_hasPubmedId: '36371895'
...