---
_id: '15132'
abstract:
- lang: eng
  text: Clustered regularly interspaced short palindromic repeats - CRISPR-associated
    protein (CRISPR-Cas) systems are a critical component of the bacterial adaptive
    immune response. Since the discovery that they can be reengineered as programmable
    RNA-guided nucleases, there has been significant interest in using these systems
    to perform diverse and precise genetic manipulations. Here, we outline recent
    advances in the mechanistic understanding of CRISPR-Cas9, how these findings have
    been leveraged in the rational redesign of Cas9 variants with altered activities,
    and how these novel tools can be exploited for biotechnology and therapeutics.
    We also discuss the potential of the ubiquitous, yet often-overlooked, multisubunit
    CRISPR effector complexes for large-scale genomic deletions. Furthermore, we highlight
    how future structural studies will bolster these technologies.
article_number: '102839'
article_processing_charge: No
article_type: review
author:
- first_name: Jack Peter Kelly
  full_name: Bravo, Jack Peter Kelly
  id: 96aecfa5-8931-11ee-af30-aa6a5d6eee0e
  last_name: Bravo
  orcid: 0000-0003-0456-0753
- first_name: Grace N
  full_name: Hibshman, Grace N
  last_name: Hibshman
- first_name: David W
  full_name: Taylor, David W
  last_name: Taylor
citation:
  ama: Bravo JPK, Hibshman GN, Taylor DW. Constructing next-generation CRISPR–Cas
    tools from structural blueprints. <i>Current Opinion in Biotechnology</i>. 2022;78.
    doi:<a href="https://doi.org/10.1016/j.copbio.2022.102839">10.1016/j.copbio.2022.102839</a>
  apa: Bravo, J. P. K., Hibshman, G. N., &#38; Taylor, D. W. (2022). Constructing
    next-generation CRISPR–Cas tools from structural blueprints. <i>Current Opinion
    in Biotechnology</i>. Elsevier. <a href="https://doi.org/10.1016/j.copbio.2022.102839">https://doi.org/10.1016/j.copbio.2022.102839</a>
  chicago: Bravo, Jack Peter Kelly, Grace N Hibshman, and David W Taylor. “Constructing
    Next-Generation CRISPR–Cas Tools from Structural Blueprints.” <i>Current Opinion
    in Biotechnology</i>. Elsevier, 2022. <a href="https://doi.org/10.1016/j.copbio.2022.102839">https://doi.org/10.1016/j.copbio.2022.102839</a>.
  ieee: J. P. K. Bravo, G. N. Hibshman, and D. W. Taylor, “Constructing next-generation
    CRISPR–Cas tools from structural blueprints,” <i>Current Opinion in Biotechnology</i>,
    vol. 78. Elsevier, 2022.
  ista: Bravo JPK, Hibshman GN, Taylor DW. 2022. Constructing next-generation CRISPR–Cas
    tools from structural blueprints. Current Opinion in Biotechnology. 78, 102839.
  mla: Bravo, Jack Peter Kelly, et al. “Constructing Next-Generation CRISPR–Cas Tools
    from Structural Blueprints.” <i>Current Opinion in Biotechnology</i>, vol. 78,
    102839, Elsevier, 2022, doi:<a href="https://doi.org/10.1016/j.copbio.2022.102839">10.1016/j.copbio.2022.102839</a>.
  short: J.P.K. Bravo, G.N. Hibshman, D.W. Taylor, Current Opinion in Biotechnology
    78 (2022).
date_created: 2024-03-20T10:41:53Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2024-10-14T12:34:11Z
day: '01'
doi: 10.1016/j.copbio.2022.102839
extern: '1'
external_id:
  pmid:
  - '36371895'
fulldoi: https://doi.org/10.1016/j.copbio.2022.102839
intvolume: '        78'
keyword:
- Biomedical Engineering
- Bioengineering
- Biotechnology
language:
- iso: eng
month: '12'
oa_version: None
pmid: 1
publication: Current Opinion in Biotechnology
publication_identifier:
  issn:
  - 0958-1669
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Constructing next-generation CRISPR–Cas tools from structural blueprints
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 78
year: '2022'
...