{"article_number":"2829","volume":13,"main_file_link":[{"url":"https://doi.org/10.1038/s41467-022-30402-8","open_access":"1"}],"day":"20","publication":"Nature Communications","year":"2022","scopus_import":"1","title":"Structural rearrangements allow nucleic acid discrimination by type I-D Cascade","article_processing_charge":"Yes","intvolume":"        13","abstract":[{"lang":"eng","text":"CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems include type I and type III. Interestingly, the type I-D interference proteins contain characteristic features of both type I and type III systems. Here, we present the structures of type I-D Cascade bound to both a double-stranded (ds)DNA and a single-stranded (ss)RNA target at 2.9 and 3.1 Å, respectively. We show that type I-D Cascade is capable of specifically binding ssRNA and reveal how PAM recognition of dsDNA targets initiates long-range structural rearrangements that likely primes Cas10d for Cas3′ binding and subsequent non-target strand DNA cleavage. These structures allow us to model how binding of the anti-CRISPR protein AcrID1 likely blocks target dsDNA binding via competitive inhibition of the DNA substrate engagement with the Cas10d active site. This work elucidates the unique mechanisms used by type I-D Cascade for discrimination of single-stranded and double stranded targets. Thus, our data supports a model for the hybrid nature of this complex with features of type III and type I systems."}],"publication_status":"published","status":"public","publisher":"Springer Nature","date_published":"2022-05-20T00:00:00Z","language":[{"iso":"eng"}],"doi":"10.1038/s41467-022-30402-8","type":"journal_article","external_id":{"pmid":["35595728"]},"extern":"1","publication_identifier":{"issn":["2041-1723"]},"month":"05","oa_version":"Published Version","date_created":"2024-03-20T10:42:05Z","_id":"15134","pmid":1,"author":[{"first_name":"Evan A.","full_name":"Schwartz, Evan A.","last_name":"Schwartz"},{"full_name":"McBride, Tess M.","first_name":"Tess M.","last_name":"McBride"},{"id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","orcid":"0000-0003-0456-0753","first_name":"Jack Peter Kelly","full_name":"Bravo, Jack Peter Kelly","last_name":"Bravo"},{"last_name":"Wrapp","full_name":"Wrapp, Daniel","first_name":"Daniel"},{"first_name":"Peter C.","full_name":"Fineran, Peter C.","last_name":"Fineran"},{"last_name":"Fagerlund","full_name":"Fagerlund, Robert D.","first_name":"Robert D."},{"last_name":"Taylor","first_name":"David W.","full_name":"Taylor, David W."}],"citation":{"short":"E.A. Schwartz, T.M. McBride, J.P.K. Bravo, D. Wrapp, P.C. Fineran, R.D. Fagerlund, D.W. Taylor, Nature Communications 13 (2022).","ieee":"E. A. Schwartz <i>et al.</i>, “Structural rearrangements allow nucleic acid discrimination by type I-D Cascade,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","ista":"Schwartz EA, McBride TM, Bravo JPK, Wrapp D, Fineran PC, Fagerlund RD, Taylor DW. 2022. Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. Nature Communications. 13, 2829.","ama":"Schwartz EA, McBride TM, Bravo JPK, et al. Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-30402-8\">10.1038/s41467-022-30402-8</a>","chicago":"Schwartz, Evan A., Tess M. McBride, Jack Peter Kelly Bravo, Daniel Wrapp, Peter C. Fineran, Robert D. Fagerlund, and David W. Taylor. “Structural Rearrangements Allow Nucleic Acid Discrimination by Type I-D Cascade.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-30402-8\">https://doi.org/10.1038/s41467-022-30402-8</a>.","apa":"Schwartz, E. A., McBride, T. M., Bravo, J. P. K., Wrapp, D., Fineran, P. C., Fagerlund, R. D., &#38; Taylor, D. W. (2022). Structural rearrangements allow nucleic acid discrimination by type I-D Cascade. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-30402-8\">https://doi.org/10.1038/s41467-022-30402-8</a>","mla":"Schwartz, Evan A., et al. “Structural Rearrangements Allow Nucleic Acid Discrimination by Type I-D Cascade.” <i>Nature Communications</i>, vol. 13, 2829, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-30402-8\">10.1038/s41467-022-30402-8</a>."},"keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"oa":1,"date_updated":"2024-06-04T06:14:28Z","article_type":"original","quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}