---
_id: '15139'
abstract:
- lang: eng
  text: Rotavirus genomes are distributed between 11 distinct RNA molecules, all of
    which must be selectively copackaged during virus assembly. This likely occurs
    through sequence-specific RNA interactions facilitated by the RNA chaperone NSP2.
    Here, we report that NSP2 autoregulates its chaperone activity through its C-terminal
    region (CTR) that promotes RNA–RNA interactions by limiting its helix-unwinding
    activity. Unexpectedly, structural proteomics data revealed that the CTR does
    not directly interact with RNA, while accelerating RNA release from NSP2. Cryo–electron
    microscopy reconstructions of an NSP2–RNA complex reveal a highly conserved acidic
    patch on the CTR, which is poised toward the bound RNA. Virus replication was
    abrogated by charge-disrupting mutations within the acidic patch but completely
    restored by charge-preserving mutations. Mechanistic similarities between NSP2
    and the unrelated bacterial RNA chaperone Hfq suggest that accelerating RNA dissociation
    while promoting intermolecular RNA interactions may be a widespread strategy of
    RNA chaperone recycling.
article_number: e2100198118
article_processing_charge: No
article_type: original
author:
- first_name: Jack Peter Kelly
  full_name: Bravo, Jack Peter Kelly
  id: 96aecfa5-8931-11ee-af30-aa6a5d6eee0e
  last_name: Bravo
  orcid: 0000-0003-0456-0753
- first_name: Kira
  full_name: Bartnik, Kira
  last_name: Bartnik
- first_name: Luca
  full_name: Venditti, Luca
  last_name: Venditti
- first_name: Julia
  full_name: Acker, Julia
  last_name: Acker
- first_name: Emma H.
  full_name: Gail, Emma H.
  last_name: Gail
- first_name: Alice
  full_name: Colyer, Alice
  last_name: Colyer
- first_name: Chen
  full_name: Davidovich, Chen
  last_name: Davidovich
- first_name: Don C.
  full_name: Lamb, Don C.
  last_name: Lamb
- first_name: Roman
  full_name: Tuma, Roman
  last_name: Tuma
- first_name: Antonio N.
  full_name: Calabrese, Antonio N.
  last_name: Calabrese
- first_name: Alexander
  full_name: Borodavka, Alexander
  last_name: Borodavka
citation:
  ama: Bravo JPK, Bartnik K, Venditti L, et al. Structural basis of rotavirus RNA
    chaperone displacement and RNA annealing. <i>Proceedings of the National Academy
    of Sciences</i>. 2021;118(41). doi:<a href="https://doi.org/10.1073/pnas.2100198118">10.1073/pnas.2100198118</a>
  apa: Bravo, J. P. K., Bartnik, K., Venditti, L., Acker, J., Gail, E. H., Colyer,
    A., … Borodavka, A. (2021). Structural basis of rotavirus RNA chaperone displacement
    and RNA annealing. <i>Proceedings of the National Academy of Sciences</i>. Proceedings
    of the National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.2100198118">https://doi.org/10.1073/pnas.2100198118</a>
  chicago: Bravo, Jack Peter Kelly, Kira Bartnik, Luca Venditti, Julia Acker, Emma
    H. Gail, Alice Colyer, Chen Davidovich, et al. “Structural Basis of Rotavirus
    RNA Chaperone Displacement and RNA Annealing.” <i>Proceedings of the National
    Academy of Sciences</i>. Proceedings of the National Academy of Sciences, 2021.
    <a href="https://doi.org/10.1073/pnas.2100198118">https://doi.org/10.1073/pnas.2100198118</a>.
  ieee: J. P. K. Bravo <i>et al.</i>, “Structural basis of rotavirus RNA chaperone
    displacement and RNA annealing,” <i>Proceedings of the National Academy of Sciences</i>,
    vol. 118, no. 41. Proceedings of the National Academy of Sciences, 2021.
  ista: Bravo JPK, Bartnik K, Venditti L, Acker J, Gail EH, Colyer A, Davidovich C,
    Lamb DC, Tuma R, Calabrese AN, Borodavka A. 2021. Structural basis of rotavirus
    RNA chaperone displacement and RNA annealing. Proceedings of the National Academy
    of Sciences. 118(41), e2100198118.
  mla: Bravo, Jack Peter Kelly, et al. “Structural Basis of Rotavirus RNA Chaperone
    Displacement and RNA Annealing.” <i>Proceedings of the National Academy of Sciences</i>,
    vol. 118, no. 41, e2100198118, Proceedings of the National Academy of Sciences,
    2021, doi:<a href="https://doi.org/10.1073/pnas.2100198118">10.1073/pnas.2100198118</a>.
  short: J.P.K. Bravo, K. Bartnik, L. Venditti, J. Acker, E.H. Gail, A. Colyer, C.
    Davidovich, D.C. Lamb, R. Tuma, A.N. Calabrese, A. Borodavka, Proceedings of the
    National Academy of Sciences 118 (2021).
date_created: 2024-03-20T10:42:45Z
date_published: 2021-10-06T00:00:00Z
date_updated: 2024-06-04T06:04:07Z
day: '06'
doi: 10.1073/pnas.2100198118
extern: '1'
external_id:
  pmid:
  - '34615715'
fulldoi: https://doi.org/10.1073/pnas.2100198118
intvolume: '       118'
issue: '41'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1073/pnas.2100198118
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Proceedings of the National Academy of Sciences
publication_identifier:
  eissn:
  - 1091-6490
  issn:
  - 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structural basis of rotavirus RNA chaperone displacement and RNA annealing
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 118
year: '2021'
...