{"year":"2021","main_file_link":[{"url":"https://doi.org/10.1016/j.isci.2021.102201","open_access":"1"}],"abstract":[{"lang":"eng","text":"We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles the type III-A CRISPR Csm effector complex, consisting of a Cas7-like (Csf2) filament intertwined with a small subunit (Cas11) filament, but the complex lacks subunits for RNA processing and target DNA cleavage. Surprisingly, instead of assembling around a CRISPR-derived RNA (crRNA), the complex assembles upon heterogeneous RNA of a regular length arranged in a pseudo-A-form configuration. These findings provide a high-resolution glimpse into the assembly and function of enigmatic type IV CRISPR systems, expanding our understanding of class I CRISPR-Cas system architecture, and suggesting a function for type IV-B RNPs that may be distinct from other class 1 CRISPR-associated systems."}],"intvolume":"        24","day":"19","date_published":"2021-03-19T00:00:00Z","publication_identifier":{"issn":["2589-0042"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Zhou, Yi","first_name":"Yi","last_name":"Zhou"},{"first_name":"Jack Peter Kelly","last_name":"Bravo","orcid":"0000-0003-0456-0753","full_name":"Bravo, Jack Peter Kelly","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e"},{"full_name":"Taylor, Hannah N.","last_name":"Taylor","first_name":"Hannah N."},{"last_name":"Steens","first_name":"Jurre A.","full_name":"Steens, Jurre A."},{"last_name":"Jackson","first_name":"Ryan N.","full_name":"Jackson, Ryan N."},{"full_name":"Staals, Raymond H.J.","first_name":"Raymond H.J.","last_name":"Staals"},{"first_name":"David W.","last_name":"Taylor","full_name":"Taylor, David W."}],"article_processing_charge":"Yes (in subscription journal)","article_number":"102201","date_created":"2024-03-20T10:43:00Z","doi":"10.1016/j.isci.2021.102201","issue":"3","citation":{"apa":"Zhou, Y., Bravo, J. P. K., Taylor, H. N., Steens, J. A., Jackson, R. N., Staals, R. H. J., &#38; Taylor, D. W. (2021). Structure of a type IV CRISPR-Cas ribonucleoprotein complex. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2021.102201\">https://doi.org/10.1016/j.isci.2021.102201</a>","short":"Y. Zhou, J.P.K. Bravo, H.N. Taylor, J.A. Steens, R.N. Jackson, R.H.J. Staals, D.W. Taylor, IScience 24 (2021).","chicago":"Zhou, Yi, Jack Peter Kelly Bravo, Hannah N. Taylor, Jurre A. Steens, Ryan N. Jackson, Raymond H.J. Staals, and David W. Taylor. “Structure of a Type IV CRISPR-Cas Ribonucleoprotein Complex.” <i>IScience</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.isci.2021.102201\">https://doi.org/10.1016/j.isci.2021.102201</a>.","ieee":"Y. Zhou <i>et al.</i>, “Structure of a type IV CRISPR-Cas ribonucleoprotein complex,” <i>iScience</i>, vol. 24, no. 3. Elsevier, 2021.","mla":"Zhou, Yi, et al. “Structure of a Type IV CRISPR-Cas Ribonucleoprotein Complex.” <i>IScience</i>, vol. 24, no. 3, 102201, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102201\">10.1016/j.isci.2021.102201</a>.","ama":"Zhou Y, Bravo JPK, Taylor HN, et al. Structure of a type IV CRISPR-Cas ribonucleoprotein complex. <i>iScience</i>. 2021;24(3). doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102201\">10.1016/j.isci.2021.102201</a>","ista":"Zhou Y, Bravo JPK, Taylor HN, Steens JA, Jackson RN, Staals RHJ, Taylor DW. 2021. Structure of a type IV CRISPR-Cas ribonucleoprotein complex. iScience. 24(3), 102201."},"status":"public","_id":"15141","publisher":"Elsevier","type":"journal_article","keyword":["Multidisciplinary"],"extern":"1","publication_status":"published","month":"03","quality_controlled":"1","oa":1,"pmid":1,"external_id":{"pmid":["33733066"]},"title":"Structure of a type IV CRISPR-Cas ribonucleoprotein complex","language":[{"iso":"eng"}],"volume":24,"oa_version":"Published Version","date_updated":"2024-06-04T05:56:45Z","publication":"iScience","article_type":"original"}