{"day":"23","title":"Structural basis for assembly of non-canonical small subunits into type I-C Cascade","date_updated":"2024-06-04T05:52:51Z","month":"11","publication":"Nature Communications","year":"2020","language":[{"iso":"eng"}],"oa":1,"author":[{"last_name":"O’Brien","full_name":"O’Brien, Roisin E.","first_name":"Roisin E."},{"full_name":"Santos, Inês C.","first_name":"Inês C.","last_name":"Santos"},{"last_name":"Wrapp","first_name":"Daniel","full_name":"Wrapp, Daniel"},{"id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","last_name":"Bravo","first_name":"Jack Peter Kelly","full_name":"Bravo, Jack Peter Kelly","orcid":"0000-0003-0456-0753"},{"last_name":"Schwartz","first_name":"Evan A.","full_name":"Schwartz, Evan A."},{"first_name":"Jennifer S.","full_name":"Brodbelt, Jennifer S.","last_name":"Brodbelt"},{"first_name":"David W.","full_name":"Taylor, David W.","last_name":"Taylor"}],"extern":"1","external_id":{"pmid":["33230133"]},"abstract":[{"text":"Bacteria and archaea employ CRISPR (clustered, regularly, interspaced, short palindromic repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and degrade foreign nucleic acids. While a myriad of CRISPR-Cas systems have been identified to date, type I-C is one of the most commonly found subtypes in nature. Interestingly, the type I-C system employs a minimal Cascade effector complex, which encodes only three unique subunits in its operon. Here, we present a 3.1 Å resolution cryo-EM structure of the <jats:italic>Desulfovibrio vulgaris</jats:italic> type I-C Cascade, revealing the molecular mechanisms that underlie RNA-directed complex assembly. We demonstrate how this minimal Cascade utilizes previously overlooked, non-canonical small subunits to stabilize R-loop formation. Furthermore, we describe putative PAM and Cas3 binding sites. These findings provide the structural basis for harnessing the type I-C Cascade as a genome-engineering tool.","lang":"eng"}],"article_processing_charge":"Yes","scopus_import":"1","doi":"10.1038/s41467-020-19785-8","article_number":"5931","publication_status":"published","volume":11,"publication_identifier":{"issn":["2041-1723"]},"publisher":"Springer Nature","oa_version":"Published Version","article_type":"original","date_created":"2024-03-20T10:43:07Z","citation":{"ama":"O’Brien RE, Santos IC, Wrapp D, et al. Structural basis for assembly of non-canonical small subunits into type I-C Cascade. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19785-8\">10.1038/s41467-020-19785-8</a>","mla":"O’Brien, Roisin E., et al. “Structural Basis for Assembly of Non-Canonical Small Subunits into Type I-C Cascade.” <i>Nature Communications</i>, vol. 11, 5931, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19785-8\">10.1038/s41467-020-19785-8</a>.","chicago":"O’Brien, Roisin E., Inês C. Santos, Daniel Wrapp, Jack Peter Kelly Bravo, Evan A. Schwartz, Jennifer S. Brodbelt, and David W. Taylor. “Structural Basis for Assembly of Non-Canonical Small Subunits into Type I-C Cascade.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19785-8\">https://doi.org/10.1038/s41467-020-19785-8</a>.","ieee":"R. E. O’Brien <i>et al.</i>, “Structural basis for assembly of non-canonical small subunits into type I-C Cascade,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","apa":"O’Brien, R. E., Santos, I. C., Wrapp, D., Bravo, J. P. K., Schwartz, E. A., Brodbelt, J. S., &#38; Taylor, D. W. (2020). Structural basis for assembly of non-canonical small subunits into type I-C Cascade. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19785-8\">https://doi.org/10.1038/s41467-020-19785-8</a>","short":"R.E. O’Brien, I.C. Santos, D. Wrapp, J.P.K. Bravo, E.A. Schwartz, J.S. Brodbelt, D.W. Taylor, Nature Communications 11 (2020).","ista":"O’Brien RE, Santos IC, Wrapp D, Bravo JPK, Schwartz EA, Brodbelt JS, Taylor DW. 2020. Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. 11, 5931."},"pmid":1,"main_file_link":[{"url":"https://doi.org/10.1038/s41467-020-19785-8","open_access":"1"}],"intvolume":"        11","_id":"15142","status":"public","quality_controlled":"1","date_published":"2020-11-23T00:00:00Z","type":"journal_article","keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}