{"publication_status":"published","date_created":"2024-03-21T07:54:44Z","month":"04","year":"2020","abstract":[{"lang":"eng","text":"Transcription factors (TFs) regulate gene expression through chromatin where nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs, we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic stem cells. We determined TF engagement throughout a nucleosome at base-pair resolution in vitro, enabling structure determination by cryo–electron microscopy at two preferred positions. Depending on motif location, OCT4 and SOX2 differentially distort nucleosomal DNA. At one position, OCT4-SOX2 removes DNA from histone H2A and histone H3; however, at an inverted motif, the TFs only induce local DNA distortions. OCT4 uses one of its two DNA-binding domains to engage DNA in both structures, reading out a partial motif. These findings explain site-specific nucleosome engagement by the pluripotency factors OCT4 and SOX2, and they reveal how TFs distort nucleosomes to access chromatinized motifs."}],"publisher":"American Association for the Advancement of Science ","language":[{"iso":"eng"}],"publication":"Science","day":"23","author":[{"first_name":"Alicia Kathleen","orcid":"0000-0002-6080-839X","last_name":"Michael","full_name":"Michael, Alicia Kathleen","id":"6437c950-2a03-11ee-914d-d6476dd7b75c"},{"first_name":"Ralph S.","last_name":"Grand","full_name":"Grand, Ralph S."},{"full_name":"Isbel, Luke","last_name":"Isbel","first_name":"Luke"},{"first_name":"Simone","last_name":"Cavadini","full_name":"Cavadini, Simone"},{"last_name":"Kozicka","first_name":"Zuzanna","full_name":"Kozicka, Zuzanna"},{"last_name":"Kempf","first_name":"Georg","full_name":"Kempf, Georg"},{"last_name":"Bunker","first_name":"Richard D.","full_name":"Bunker, Richard D."},{"first_name":"Andreas D.","last_name":"Schenk","full_name":"Schenk, Andreas D."},{"last_name":"Graff-Meyer","first_name":"Alexandra","full_name":"Graff-Meyer, Alexandra"},{"full_name":"Pathare, Ganesh R.","last_name":"Pathare","first_name":"Ganesh R."},{"full_name":"Weiss, Joscha","last_name":"Weiss","first_name":"Joscha"},{"last_name":"Matsumoto","first_name":"Syota","full_name":"Matsumoto, Syota"},{"full_name":"Burger, Lukas","first_name":"Lukas","last_name":"Burger"},{"full_name":"Schübeler, Dirk","last_name":"Schübeler","first_name":"Dirk"},{"full_name":"Thomä, Nicolas H.","last_name":"Thomä","first_name":"Nicolas H."}],"issue":"6498","page":"1460-1465","publication_identifier":{"eissn":["1095-9203"],"issn":["0036-8075"]},"article_processing_charge":"No","title":"Mechanisms of OCT4-SOX2 motif readout on nucleosomes","citation":{"ieee":"A. K. Michael <i>et al.</i>, “Mechanisms of OCT4-SOX2 motif readout on nucleosomes,” <i>Science</i>, vol. 368, no. 6498. American Association for the Advancement of Science , pp. 1460–1465, 2020.","short":"A.K. Michael, R.S. Grand, L. Isbel, S. Cavadini, Z. Kozicka, G. Kempf, R.D. Bunker, A.D. Schenk, A. Graff-Meyer, G.R. Pathare, J. Weiss, S. Matsumoto, L. Burger, D. Schübeler, N.H. Thomä, Science 368 (2020) 1460–1465.","apa":"Michael, A. K., Grand, R. S., Isbel, L., Cavadini, S., Kozicka, Z., Kempf, G., … Thomä, N. H. (2020). Mechanisms of OCT4-SOX2 motif readout on nucleosomes. <i>Science</i>. American Association for the Advancement of Science . <a href=\"https://doi.org/10.1126/science.abb0074\">https://doi.org/10.1126/science.abb0074</a>","ama":"Michael AK, Grand RS, Isbel L, et al. Mechanisms of OCT4-SOX2 motif readout on nucleosomes. <i>Science</i>. 2020;368(6498):1460-1465. doi:<a href=\"https://doi.org/10.1126/science.abb0074\">10.1126/science.abb0074</a>","chicago":"Michael, Alicia K., Ralph S. Grand, Luke Isbel, Simone Cavadini, Zuzanna Kozicka, Georg Kempf, Richard D. Bunker, et al. “Mechanisms of OCT4-SOX2 Motif Readout on Nucleosomes.” <i>Science</i>. American Association for the Advancement of Science , 2020. <a href=\"https://doi.org/10.1126/science.abb0074\">https://doi.org/10.1126/science.abb0074</a>.","mla":"Michael, Alicia K., et al. “Mechanisms of OCT4-SOX2 Motif Readout on Nucleosomes.” <i>Science</i>, vol. 368, no. 6498, American Association for the Advancement of Science , 2020, pp. 1460–65, doi:<a href=\"https://doi.org/10.1126/science.abb0074\">10.1126/science.abb0074</a>.","ista":"Michael AK, Grand RS, Isbel L, Cavadini S, Kozicka Z, Kempf G, Bunker RD, Schenk AD, Graff-Meyer A, Pathare GR, Weiss J, Matsumoto S, Burger L, Schübeler D, Thomä NH. 2020. Mechanisms of OCT4-SOX2 motif readout on nucleosomes. Science. 368(6498), 1460–1465."},"intvolume":"       368","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-03-25T12:29:34Z","type":"journal_article","doi":"10.1126/science.abb0074","oa_version":"None","article_type":"original","quality_controlled":"1","status":"public","volume":368,"date_published":"2020-04-23T00:00:00Z","_id":"15152","scopus_import":"1","extern":"1"}