---
OA_place: repository
OA_type: green
_id: '15251'
abstract:
- lang: eng
text: Brassinosteroids are steroidal phytohormones that regulate plant development
and physiology, including adaptation to environmental stresses. Brassinosteroids
are synthesized in the cell interior but bind receptors at the cell surface, necessitating
a yet to be identified export mechanism. Here, we show that a member of the ATP-binding
cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid
exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound
states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis
activity, and transport assays showed that ABCB19 transports brassinosteroids.
In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate
brassinosteroid responses. Our results uncover an elusive export mechanism for
bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
acknowledgement: "We thank the Cryo-EM Center of the University of Science and Technology
of China for the EM facility support, Y. Yin (Iowa State University, Ames, IA, USA)
for providing the anti-BES1 antibody, Y. Gao and all other staff members for their
technical support on cryo-EM data collection, S. Vanneste (VIB, Ghent University,
Ghent, Belgium) for useful discussions, and M. De Cock for help in preparing the
manuscript.\r\nThis work was supported by the National Natural Science Foundation
of China (grants 31870732 and 32322041 to L.S., grant 31900885 to X.L., and grant
32321001 to L.S.); the Strategic Priority Research Program of the Chinese Academy
of Sciences (grant XDB37020103 to L.S.); the Natural Science Foundation of Anhui
Province (grant 2008085MC90 to X.L. and grant 2008085J15 to L.S.); Fundamental Research
Funds for the Central Universities (grant WK9100000031 to L.S.); USTC Research Funds
of the Double First-Class Initiative (grant YD9100002004 to L.S. and grant YD9100002020
to X.L.); Research Foundation-Flanders (grant G002121N to E.R. and postdoctoral
fellowships 12R7822N and 12R7819N to N.V.); and Chinese Scholarship Council predoctoral
fellowships (Y.W. and H.Z.). L.S. is supported by an Outstanding Young Scholar Award
from the Qiu Shi Science and Technologies Foundation and a Young Scholar Award from
the Cyrus Tang Foundation."
article_processing_charge: No
article_type: original
author:
- first_name: Wei
full_name: Ying, Wei
last_name: Ying
- first_name: Yaowei
full_name: Wang, Yaowei
last_name: Wang
- first_name: Hong
full_name: Wei, Hong
last_name: Wei
- first_name: Yongming
full_name: Luo, Yongming
last_name: Luo
- first_name: Qian
full_name: Ma, Qian
last_name: Ma
- first_name: Heyuan
full_name: Zhu, Heyuan
last_name: Zhu
- first_name: Hilde
full_name: Janssens, Hilde
last_name: Janssens
- first_name: Nemanja
full_name: Vukašinović, Nemanja
last_name: Vukašinović
- first_name: Miroslav
full_name: Kvasnica, Miroslav
last_name: Kvasnica
- first_name: Johan M.
full_name: Winne, Johan M.
last_name: Winne
- first_name: Yongxiang
full_name: Gao, Yongxiang
last_name: Gao
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Xin
full_name: Liu, Xin
last_name: Liu
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Linfeng
full_name: Sun, Linfeng
last_name: Sun
citation:
ama: Ying W, Wang Y, Wei H, et al. Structure and function of the Arabidopsis ABC
transporter ABCB19 in brassinosteroid export. Science. 2024;383(6689):eadj4591.
doi:10.1126/science.adj4591
apa: Ying, W., Wang, Y., Wei, H., Luo, Y., Ma, Q., Zhu, H., … Sun, L. (2024). Structure
and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.adj4591
chicago: Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde
Janssens, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19
in Brassinosteroid Export.” Science. American Association for the Advancement
of Science, 2024. https://doi.org/10.1126/science.adj4591.
ieee: W. Ying et al., “Structure and function of the Arabidopsis ABC transporter
ABCB19 in brassinosteroid export,” Science, vol. 383, no. 6689. American
Association for the Advancement of Science, p. eadj4591, 2024.
ista: Ying W, Wang Y, Wei H, Luo Y, Ma Q, Zhu H, Janssens H, Vukašinović N, Kvasnica
M, Winne JM, Gao Y, Tan S, Friml J, Liu X, Russinova E, Sun L. 2024. Structure
and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Science. 383(6689), eadj4591.
mla: Ying, Wei, et al. “Structure and Function of the Arabidopsis ABC Transporter
ABCB19 in Brassinosteroid Export.” Science, vol. 383, no. 6689, American
Association for the Advancement of Science, 2024, p. eadj4591, doi:10.1126/science.adj4591.
short: W. Ying, Y. Wang, H. Wei, Y. Luo, Q. Ma, H. Zhu, H. Janssens, N. Vukašinović,
M. Kvasnica, J.M. Winne, Y. Gao, S. Tan, J. Friml, X. Liu, E. Russinova, L. Sun,
Science 383 (2024) eadj4591.
date_created: 2024-03-31T22:01:12Z
date_published: 2024-03-22T00:00:00Z
date_updated: 2025-09-04T13:19:48Z
day: '22'
department:
- _id: JiFr
doi: 10.1126/science.adj4591
external_id:
isi:
- '001252955200028'
pmid:
- '38513023'
intvolume: ' 383'
isi: 1
issue: '6689'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://hdl.handle.net/1854/LU-01HTMBK3P3PPNB73YFCMFXP3ZZ
month: '03'
oa: 1
oa_version: Submitted Version
page: eadj4591
pmid: 1
publication: Science
publication_identifier:
eissn:
- 1095-9203
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid
export
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 383
year: '2024'
...