{"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png"},"oa":1,"type":"journal_article","doi":"10.1242/dev.196121","status":"public","article_number":"dev196121.","article_processing_charge":"No","date_updated":"2024-04-03T14:00:33Z","file":[{"creator":"dernst","access_level":"open_access","date_updated":"2024-04-03T13:58:51Z","file_id":"15290","checksum":"6d0533fe9c712448b3f9feb15e05ec4b","relation":"main_file","file_size":16258500,"date_created":"2024-04-03T13:58:51Z","success":1,"file_name":"2021_CompanyBiologists_Vinter.pdf","content_type":"application/pdf"}],"year":"2021","date_published":"2021-09-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"01","file_date_updated":"2024-04-03T13:58:51Z","has_accepted_license":"1","abstract":[{"text":"The Hunchback (Hb) transcription factor is crucial for anterior-posterior patterning of the Drosophila embryo. The maternal hb mRNA acts as a paradigm for translational regulation due to its repression in the posterior of the embryo. However, little is known about the translatability of zygotically transcribed hb mRNAs. Here, we adapt the SunTag system, developed for imaging translation at single-mRNA resolution in tissue culture cells, to the Drosophila embryo to study the translation dynamics of zygotic hb mRNAs. Using single-molecule imaging in fixed and live embryos, we provide evidence for translational repression of zygotic SunTag-hb mRNAs. Whereas the proportion of SunTag-hb mRNAs translated is initially uniform, translation declines from the anterior over time until it becomes restricted to a posterior band in the expression domain. We discuss how regulated hb mRNA translation may help establish the sharp Hb expression boundary, which is a model for precision and noise during developmental patterning. Overall, our data show how use of the SunTag method on fixed and live embryos is a powerful combination for elucidating spatiotemporal regulation of mRNA translation in Drosophila.","lang":"eng"}],"issue":"18","department":[{"_id":"AnKi"}],"date_created":"2024-04-03T07:26:41Z","external_id":{"pmid":["33722899 "]},"publication":"Development","oa_version":"Published Version","month":"09","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"quality_controlled":"1","article_type":"original","language":[{"iso":"eng"}],"pmid":1,"scopus_import":"1","_id":"15262","citation":{"mla":"Vinter, Daisy J., et al. “Dynamics of Hunchback Translation in Real-Time and at Single-MRNA Resolution in the Drosophila Embryo.” <i>Development</i>, vol. 148, no. 18, dev196121., The Company of Biologists, 2021, doi:<a href=\"https://doi.org/10.1242/dev.196121\">10.1242/dev.196121</a>.","apa":"Vinter, D. J., Hoppe, C., Minchington, T., Sutcliffe, C., &#38; Ashe, H. L. (2021). Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.196121\">https://doi.org/10.1242/dev.196121</a>","ieee":"D. J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, and H. L. Ashe, “Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo,” <i>Development</i>, vol. 148, no. 18. The Company of Biologists, 2021.","chicago":"Vinter, Daisy J., Caroline Hoppe, Thomas Minchington, Catherine Sutcliffe, and Hilary L. Ashe. “Dynamics of Hunchback Translation in Real-Time and at Single-MRNA Resolution in the Drosophila Embryo.” <i>Development</i>. The Company of Biologists, 2021. <a href=\"https://doi.org/10.1242/dev.196121\">https://doi.org/10.1242/dev.196121</a>.","short":"D.J. Vinter, C. Hoppe, T. Minchington, C. Sutcliffe, H.L. Ashe, Development 148 (2021).","ista":"Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. 2021. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Development. 148(18), dev196121.","ama":"Vinter DJ, Hoppe C, Minchington T, Sutcliffe C, Ashe HL. Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. <i>Development</i>. 2021;148(18). doi:<a href=\"https://doi.org/10.1242/dev.196121\">10.1242/dev.196121</a>"},"publication_status":"published","title":"Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo","ddc":["570"],"volume":148,"intvolume":"       148","publisher":"The Company of Biologists","author":[{"first_name":"Daisy J.","full_name":"Vinter, Daisy J.","last_name":"Vinter"},{"last_name":"Hoppe","full_name":"Hoppe, Caroline","first_name":"Caroline"},{"id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f","last_name":"Minchington","full_name":"Minchington, Thomas","first_name":"Thomas"},{"full_name":"Sutcliffe, Catherine","first_name":"Catherine","last_name":"Sutcliffe"},{"first_name":"Hilary L.","full_name":"Ashe, Hilary L.","last_name":"Ashe"}],"keyword":["Developmental Biology","Molecular Biology"]}