{"volume":9,"article_number":"647063","intvolume":"         9","file_date_updated":"2024-04-03T14:08:05Z","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"oa_version":"Published Version","publisher":"Frontiers Media","has_accepted_license":"1","doi":"10.3389/fcell.2021.647063","day":"01","article_processing_charge":"No","external_id":{"pmid":["34336818"]},"publication_identifier":{"issn":["2296-634X"]},"_id":"15264","author":[{"full_name":"Deretic, Nikola","last_name":"Deretic","first_name":"Nikola"},{"last_name":"Bolger-Munro","id":"516F03FA-93A3-11EA-A7C5-D6BE3DDC885E","first_name":"Madison","orcid":"0000-0002-8176-4824","full_name":"Bolger-Munro, Madison"},{"first_name":"Kate","last_name":"Choi","full_name":"Choi, Kate"},{"full_name":"Abraham, Libin","last_name":"Abraham","first_name":"Libin"},{"full_name":"Gold, Michael R.","first_name":"Michael R.","last_name":"Gold"}],"scopus_import":"1","date_created":"2024-04-03T07:34:08Z","month":"07","type":"journal_article","title":"The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"quality_controlled":"1","status":"public","citation":{"ista":"Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. 2021. The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. Frontiers in Cell and Developmental Biology. 9, 647063.","apa":"Deretic, N., Bolger-Munro, M., Choi, K., Abraham, L., &#38; Gold, M. R. (2021). The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fcell.2021.647063\">https://doi.org/10.3389/fcell.2021.647063</a>","ama":"Deretic N, Bolger-Munro M, Choi K, Abraham L, Gold MR. The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse. <i>Frontiers in Cell and Developmental Biology</i>. 2021;9. doi:<a href=\"https://doi.org/10.3389/fcell.2021.647063\">10.3389/fcell.2021.647063</a>","ieee":"N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, and M. R. Gold, “The actin-disassembly protein glia maturation factor γ enhances actin remodeling and B cell antigen receptor signaling at the immune synapse,” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9. Frontiers Media, 2021.","chicago":"Deretic, Nikola, Madison Bolger-Munro, Kate Choi, Libin Abraham, and Michael R. Gold. “The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>. Frontiers Media, 2021. <a href=\"https://doi.org/10.3389/fcell.2021.647063\">https://doi.org/10.3389/fcell.2021.647063</a>.","mla":"Deretic, Nikola, et al. “The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.” <i>Frontiers in Cell and Developmental Biology</i>, vol. 9, 647063, Frontiers Media, 2021, doi:<a href=\"https://doi.org/10.3389/fcell.2021.647063\">10.3389/fcell.2021.647063</a>.","short":"N. Deretic, M. Bolger-Munro, K. Choi, L. Abraham, M.R. Gold, Frontiers in Cell and Developmental Biology 9 (2021)."},"keyword":["Cell Biology","Developmental Biology"],"date_updated":"2024-04-03T14:10:25Z","abstract":[{"lang":"eng","text":"Signaling by the B cell antigen receptor (BCR) initiates actin remodeling. The assembly of branched actin networks that are nucleated by the Arp2/3 complex exert outward force on the plasma membrane, allowing B cells to form membrane protrusions that can scan the surface of antigen-presenting cells (APCs). The resulting Arp2/3 complex-dependent actin retrograde flow promotes the centripetal movement and progressive coalescence of BCR microclusters, which amplifies BCR signaling. Glia maturation factor γ (GMFγ) is an actin disassembly-protein that releases Arp2/3 complex-nucleated actin filaments from actin networks. By doing so, GMFγ could either oppose the actions of the Arp2/3 complex or support Arp2/3 complex-nucleated actin polymerization by contributing to the recycling of actin monomers and Arp2/3 complexes. We now show that reducing the levels of GMFγ in human B cell lines via transfection with a specific siRNA impairs the ability of B cells to spread on antigen-coated surfaces, decreases the velocity of actin retrograde flow, diminishes the coalescence of BCR microclusters into a central cluster at the B cell-APC contact site, and decreases APC-induced BCR signaling. These effects of depleting GMFγ are similar to what occurs when the Arp2/3 complex is inhibited. This suggests that GMFγ cooperates with the Arp2/3 complex to support BCR-induced actin remodeling and amplify BCR signaling at the immune synapse."}],"ddc":["570"],"pmid":1,"publication_status":"published","file":[{"creator":"dernst","date_updated":"2024-04-03T14:08:05Z","file_id":"15291","file_name":"2021_Frontiers_Deretic.pdf","date_created":"2024-04-03T14:08:05Z","content_type":"application/pdf","success":1,"file_size":7430029,"access_level":"open_access","checksum":"f6330b5c6718d6780383c0300fd4ef12","relation":"main_file"}],"publication":"Frontiers in Cell and Developmental Biology","year":"2021","oa":1,"language":[{"iso":"eng"}],"date_published":"2021-07-01T00:00:00Z"}