---
res:
  bibo_abstract:
  - In the eukaryotic nucleus, heterochromatin forms highly condensed, visible foci
    known as heterochromatin foci (HF). These HF are enriched with linker histone
    H1, a key player in heterochromatin condensation and silencing. However, it is
    unknown how H1 aggregates HF and condenses heterochromatin. In this study, we
    established that H1 facilitates heterochromatin condensation by enhancing inter-
    and intrachromosomal interactions between and within heterochromatic regions of
    the Arabidopsis (Arabidopsis thaliana) genome. We demonstrated that H1 drives
    HF formation via phase separation, which requires its C-terminal intrinsically
    disordered region (C-IDR). A truncated H1 lacking the C-IDR fails to form foci
    or recover HF in the h1 mutant background, whereas C-IDR with a short stretch
    of the globular domain (18 out of 71 amino acids) is sufficient to rescue both
    defects. In addition, C-IDR is essential for H1's roles in regulating nucleosome
    repeat length and DNA methylation in Arabidopsis, indicating that phase separation
    capability is required for chromatin functions of H1. Our data suggest that bacterial
    H1-like proteins, which have been shown to condense DNA, are intrinsically disordered
    and capable of mediating phase separation. Therefore, we propose that phase separation
    mediated by H1 or H1-like proteins may represent an ancient mechanism for condensing
    chromatin and DNA.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Shengbo
      foaf_name: He, Shengbo
      foaf_surname: He
  - foaf_Person:
      foaf_givenName: Yiming
      foaf_name: Yu, Yiming
      foaf_surname: Yu
      foaf_workInfoHomepage: http://www.librecat.org/personId=318e643b-8b61-11ed-b69e-aafa103ec8dd
  - foaf_Person:
      foaf_givenName: Liang
      foaf_name: Wang, Liang
      foaf_surname: Wang
  - foaf_Person:
      foaf_givenName: Jingyi
      foaf_name: Zhang, Jingyi
      foaf_surname: Zhang
  - foaf_Person:
      foaf_givenName: Zhengyong
      foaf_name: Bai, Zhengyong
      foaf_surname: Bai
  - foaf_Person:
      foaf_givenName: Guohong
      foaf_name: Li, Guohong
      foaf_surname: Li
  - foaf_Person:
      foaf_givenName: Pilong
      foaf_name: Li, Pilong
      foaf_surname: Li
  - foaf_Person:
      foaf_givenName: Xiaoqi
      foaf_name: Feng, Xiaoqi
      foaf_surname: Feng
      foaf_workInfoHomepage: http://www.librecat.org/personId=e0164712-22ee-11ed-b12a-d80fcdf35958
    orcid: 0000-0002-4008-1234
  bibo_doi: 10.1093/plcell/koae034
  bibo_issue: '5'
  bibo_volume: 36
  dct_date: 2024^xs_gYear
  dct_identifier:
  - UT:001180817000001
  dct_isPartOf:
  - http://id.crossref.org/issn/1532-298X
  dct_language: eng
  dct_publisher: Oxford University Press@
  dct_title: Linker histone H1 drives heterochromatin condensation via phase separation
    in Arabidopsis@
  fabio_hasPubmedId: '38309957'
...