---
OA_place: publisher
OA_type: hybrid
_id: '15375'
abstract:
- lang: eng
  text: In the eukaryotic nucleus, heterochromatin forms highly condensed, visible
    foci known as heterochromatin foci (HF). These HF are enriched with linker histone
    H1, a key player in heterochromatin condensation and silencing. However, it is
    unknown how H1 aggregates HF and condenses heterochromatin. In this study, we
    established that H1 facilitates heterochromatin condensation by enhancing inter-
    and intrachromosomal interactions between and within heterochromatic regions of
    the Arabidopsis (Arabidopsis thaliana) genome. We demonstrated that H1 drives
    HF formation via phase separation, which requires its C-terminal intrinsically
    disordered region (C-IDR). A truncated H1 lacking the C-IDR fails to form foci
    or recover HF in the h1 mutant background, whereas C-IDR with a short stretch
    of the globular domain (18 out of 71 amino acids) is sufficient to rescue both
    defects. In addition, C-IDR is essential for H1's roles in regulating nucleosome
    repeat length and DNA methylation in Arabidopsis, indicating that phase separation
    capability is required for chromatin functions of H1. Our data suggest that bacterial
    H1-like proteins, which have been shown to condense DNA, are intrinsically disordered
    and capable of mediating phase separation. Therefore, we propose that phase separation
    mediated by H1 or H1-like proteins may represent an ancient mechanism for condensing
    chromatin and DNA.
acknowledged_ssus:
- _id: Bio
- _id: ScienComp
acknowledgement: "This work was funded by ISTA core support (Y.Y. and X.F.) and grants
  from the National Natural Science Foundation of China (31871443 to L.W. and P.L.;
  32100417 to L.W.).\r\nWe thank the ISTA Imaging and Optics Facility for assistance
  with microscopy and the ISTA Scientific Computing Facility for high-performance
  computing resources."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Shengbo
  full_name: He, Shengbo
  last_name: He
- first_name: Yiming
  full_name: Yu, Yiming
  id: 318e643b-8b61-11ed-b69e-aafa103ec8dd
  last_name: Yu
- first_name: Liang
  full_name: Wang, Liang
  last_name: Wang
- first_name: Jingyi
  full_name: Zhang, Jingyi
  last_name: Zhang
- first_name: Zhengyong
  full_name: Bai, Zhengyong
  last_name: Bai
- first_name: Guohong
  full_name: Li, Guohong
  last_name: Li
- first_name: Pilong
  full_name: Li, Pilong
  last_name: Li
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
citation:
  ama: He S, Yu Y, Wang L, et al. Linker histone H1 drives heterochromatin condensation
    via phase separation in Arabidopsis. <i>The Plant Cell</i>. 2024;36(5):1829-1843.
    doi:<a href="https://doi.org/10.1093/plcell/koae034">10.1093/plcell/koae034</a>
  apa: He, S., Yu, Y., Wang, L., Zhang, J., Bai, Z., Li, G., … Feng, X. (2024). Linker
    histone H1 drives heterochromatin condensation via phase separation in Arabidopsis.
    <i>The Plant Cell</i>. Oxford University Press. <a href="https://doi.org/10.1093/plcell/koae034">https://doi.org/10.1093/plcell/koae034</a>
  chicago: He, Shengbo, Yiming Yu, Liang Wang, Jingyi Zhang, Zhengyong Bai, Guohong
    Li, Pilong Li, and Xiaoqi Feng. “Linker Histone H1 Drives Heterochromatin Condensation
    via Phase Separation in Arabidopsis.” <i>The Plant Cell</i>. Oxford University
    Press, 2024. <a href="https://doi.org/10.1093/plcell/koae034">https://doi.org/10.1093/plcell/koae034</a>.
  ieee: S. He <i>et al.</i>, “Linker histone H1 drives heterochromatin condensation
    via phase separation in Arabidopsis,” <i>The Plant Cell</i>, vol. 36, no. 5. Oxford
    University Press, pp. 1829–1843, 2024.
  ista: He S, Yu Y, Wang L, Zhang J, Bai Z, Li G, Li P, Feng X. 2024. Linker histone
    H1 drives heterochromatin condensation via phase separation in Arabidopsis. The
    Plant Cell. 36(5), 1829–1843.
  mla: He, Shengbo, et al. “Linker Histone H1 Drives Heterochromatin Condensation
    via Phase Separation in Arabidopsis.” <i>The Plant Cell</i>, vol. 36, no. 5, Oxford
    University Press, 2024, pp. 1829–43, doi:<a href="https://doi.org/10.1093/plcell/koae034">10.1093/plcell/koae034</a>.
  short: S. He, Y. Yu, L. Wang, J. Zhang, Z. Bai, G. Li, P. Li, X. Feng, The Plant
    Cell 36 (2024) 1829–1843.
corr_author: '1'
date_created: 2024-05-12T22:01:01Z
date_published: 2024-05-01T00:00:00Z
date_updated: 2025-09-08T07:21:17Z
day: '01'
ddc:
- '580'
department:
- _id: XiFe
doi: 10.1093/plcell/koae034
external_id:
  isi:
  - '001180817000001'
  pmid:
  - '38309957'
file:
- access_level: open_access
  checksum: eed76c848fe3d8fe9a53943181aaa53c
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  creator: dernst
  date_created: 2025-04-23T07:43:12Z
  date_updated: 2025-04-23T07:43:12Z
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  file_name: 2024_PlantCell_He.pdf
  file_size: 50791962
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  success: 1
file_date_updated: 2025-04-23T07:43:12Z
has_accepted_license: '1'
intvolume: '        36'
isi: 1
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1829-1843
pmid: 1
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Linker histone H1 drives heterochromatin condensation via phase separation
  in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 36
year: '2024'
...