---
res:
  bibo_abstract:
  - 'Cell division in prokaryotes and eukaryotes is commonly initiated by the well-controlled
    binding of proteins to the cytoplasmic side of the cell membrane. However, a precise
    characterization of the spatiotemporal dynamics of membrane-bound proteins is
    often difficult to achieve in vivo. Here, we present protocols for the use of
    supported lipid bilayers to rebuild the cytokinetic machineries of cells with
    greatly different dimensions: the bacterium Escherichia coli and eggs of the vertebrate
    Xenopus laevis. Combined with total internal reflection fluorescence microscopy,
    these experimental setups allow for precise quantitative analyses of membrane-bound
    proteins. The protocols described to obtain glass-supported membranes from bacterial
    and vertebrate lipids can be used as starting points for other reconstitution
    experiments. We believe that similar biochemical assays will be instrumental to
    study the biochemistry and biophysics underlying a variety of complex cellular
    tasks, such as signaling, vesicle trafficking, and cell motility.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Phuong
      foaf_name: Nguyen, Phuong
      foaf_surname: Nguyen
  - foaf_Person:
      foaf_givenName: Christine
      foaf_name: Field, Christine
      foaf_surname: Field
  - foaf_Person:
      foaf_givenName: Aaron
      foaf_name: Groen, Aaron
      foaf_surname: Groen
  - foaf_Person:
      foaf_givenName: Timothy
      foaf_name: Mitchison, Timothy
      foaf_surname: Mitchison
  - foaf_Person:
      foaf_givenName: Martin
      foaf_name: Loose, Martin
      foaf_surname: Loose
      foaf_workInfoHomepage: http://www.librecat.org/personId=462D4284-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7309-9724
  bibo_doi: 10.1016/bs.mcb.2015.01.007
  bibo_volume: 128
  dct_date: 2015^xs_gYear
  dct_identifier:
  - UT:000370490800013
  dct_language: eng
  dct_publisher: Academic Press@
  dct_title: Using supported bilayers to study the spatiotemporal organization of
    membrane-bound proteins@
  fabio_hasPubmedId: '25997350'
...