---
_id: '1544'
abstract:
- lang: eng
  text: 'Cell division in prokaryotes and eukaryotes is commonly initiated by the
    well-controlled binding of proteins to the cytoplasmic side of the cell membrane.
    However, a precise characterization of the spatiotemporal dynamics of membrane-bound
    proteins is often difficult to achieve in vivo. Here, we present protocols for
    the use of supported lipid bilayers to rebuild the cytokinetic machineries of
    cells with greatly different dimensions: the bacterium Escherichia coli and eggs
    of the vertebrate Xenopus laevis. Combined with total internal reflection fluorescence
    microscopy, these experimental setups allow for precise quantitative analyses
    of membrane-bound proteins. The protocols described to obtain glass-supported
    membranes from bacterial and vertebrate lipids can be used as starting points
    for other reconstitution experiments. We believe that similar biochemical assays
    will be instrumental to study the biochemistry and biophysics underlying a variety
    of complex cellular tasks, such as signaling, vesicle trafficking, and cell motility.'
article_processing_charge: No
author:
- first_name: Phuong
  full_name: Nguyen, Phuong
  last_name: Nguyen
- first_name: Christine
  full_name: Field, Christine
  last_name: Field
- first_name: Aaron
  full_name: Groen, Aaron
  last_name: Groen
- first_name: Timothy
  full_name: Mitchison, Timothy
  last_name: Mitchison
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
citation:
  ama: 'Nguyen P, Field C, Groen A, Mitchison T, Loose M. Using supported bilayers
    to study the spatiotemporal organization of membrane-bound proteins. In: <i>Building
    a Cell from Its Components Parts</i>. Vol 128. Academic Press; 2015:223-241. doi:<a
    href="https://doi.org/10.1016/bs.mcb.2015.01.007">10.1016/bs.mcb.2015.01.007</a>'
  apa: Nguyen, P., Field, C., Groen, A., Mitchison, T., &#38; Loose, M. (2015). Using
    supported bilayers to study the spatiotemporal organization of membrane-bound
    proteins. In <i>Building a Cell from its Components Parts</i> (Vol. 128, pp. 223–241).
    Academic Press. <a href="https://doi.org/10.1016/bs.mcb.2015.01.007">https://doi.org/10.1016/bs.mcb.2015.01.007</a>
  chicago: Nguyen, Phuong, Christine Field, Aaron Groen, Timothy Mitchison, and Martin
    Loose. “Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane-Bound
    Proteins.” In <i>Building a Cell from Its Components Parts</i>, 128:223–41. Academic
    Press, 2015. <a href="https://doi.org/10.1016/bs.mcb.2015.01.007">https://doi.org/10.1016/bs.mcb.2015.01.007</a>.
  ieee: P. Nguyen, C. Field, A. Groen, T. Mitchison, and M. Loose, “Using supported
    bilayers to study the spatiotemporal organization of membrane-bound proteins,”
    in <i>Building a Cell from its Components Parts</i>, vol. 128, Academic Press,
    2015, pp. 223–241.
  ista: 'Nguyen P, Field C, Groen A, Mitchison T, Loose M. 2015.Using supported bilayers
    to study the spatiotemporal organization of membrane-bound proteins. In: Building
    a Cell from its Components Parts. vol. 128, 223–241.'
  mla: Nguyen, Phuong, et al. “Using Supported Bilayers to Study the Spatiotemporal
    Organization of Membrane-Bound Proteins.” <i>Building a Cell from Its Components
    Parts</i>, vol. 128, Academic Press, 2015, pp. 223–41, doi:<a href="https://doi.org/10.1016/bs.mcb.2015.01.007">10.1016/bs.mcb.2015.01.007</a>.
  short: P. Nguyen, C. Field, A. Groen, T. Mitchison, M. Loose, in:, Building a Cell
    from Its Components Parts, Academic Press, 2015, pp. 223–241.
corr_author: '1'
date_created: 2018-12-11T11:52:38Z
date_published: 2015-04-08T00:00:00Z
date_updated: 2025-09-29T11:03:06Z
day: '08'
department:
- _id: MaLo
doi: 10.1016/bs.mcb.2015.01.007
external_id:
  isi:
  - '000370490800013'
  pmid:
  - '25997350'
intvolume: '       128'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578691/
month: '04'
oa: 1
oa_version: Submitted Version
page: 223 - 241
pmid: 1
publication: Building a Cell from its Components Parts
publication_status: published
publisher: Academic Press
publist_id: '5627'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Using supported bilayers to study the spatiotemporal organization of membrane-bound
  proteins
type: book_chapter
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 128
year: '2015'
...