---
_id: '1557'
abstract:
- lang: eng
  text: γ-Aminobutyric acid (GABA)- and glycine-mediated hyperpolarizing inhibition
    is associated with a chloride influx that depends on the inwardly directed chloride
    electrochemical gradient. In neurons, the extrusion of chloride from the cytosol
    primarily depends on the expression of an isoform of potassium-chloride cotransporters
    (KCC2s). KCC2 is crucial in the regulation of the inhibitory tone of neural circuits,
    including pain processing neural assemblies. Thus we investigated the cellular
    distribution of KCC2 in neurons underlying pain processing in the superficial
    spinal dorsal horn of rats by using high-resolution immunocytochemical methods.
    We demonstrated that perikarya and dendrites widely expressed KCC2, but axon terminals
    proved to be negative for KCC2. In single ultrathin sections, silver deposits
    labeling KCC2 molecules showed different densities on the surface of dendritic
    profiles, some of which were negative for KCC2. In freeze fracture replicas and
    tissue sections double stained for the β3-subunit of GABAA receptors and KCC2,
    GABAA receptors were revealed on dendritic segments with high and also with low
    KCC2 densities. By measuring the distances between spots immunoreactive for gephyrin
    (a scaffolding protein of GABAA and glycine receptors) and KCC2 on the surface
    of neurokinin 1 (NK1) receptor-immunoreactive dendrites, we found that gephyrin-immunoreactive
    spots were located at various distances from KCC2 cotransporters; 5.7 % of them
    were recovered in the middle of 4-10-μm-long dendritic segments that were free
    of KCC2 immunostaining. The variable local densities of KCC2 may result in variable
    postsynaptic potentials evoked by the activation of GABAA and glycine receptors
    along the dendrites of spinal neurons.
acknowledgement: "Funded by:\r\nHungarian Academy of Sciences. Grant Number: MTA-TKI
  242\r\nHungarian Brain Research Program. Grant Number: KTIA_NAP_13-1-2013-0001\r\nSolution
  Oriented Research for Science and Technology from the Japan Science and Technology
  Agency Japanese Ministry of Education, Culture, Sports, Science and Technology"
article_processing_charge: No
author:
- first_name: Fariba
  full_name: Javdani, Fariba
  last_name: Javdani
- first_name: Krisztina
  full_name: Holló, Krisztina
  last_name: Holló
- first_name: Krisztina
  full_name: Hegedűs, Krisztina
  last_name: Hegedűs
- first_name: Gréta
  full_name: Kis, Gréta
  last_name: Kis
- first_name: Zoltán
  full_name: Hegyi, Zoltán
  last_name: Hegyi
- first_name: Klaudia
  full_name: Dócs, Klaudia
  last_name: Dócs
- first_name: Yu
  full_name: Kasugai, Yu
  last_name: Kasugai
- first_name: Yugo
  full_name: Fukazawa, Yugo
  last_name: Fukazawa
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Miklós
  full_name: Antal, Miklós
  last_name: Antal
citation:
  ama: Javdani F, Holló K, Hegedűs K, et al. Differential expression patterns of K+Cl-
    cotransporter 2 in neurons within the superficial spinal dorsal horn of rats.
    <i>Journal of Comparative Neurology</i>. 2015;523(13):1967-1983. doi:<a href="https://doi.org/10.1002/cne.23774">10.1002/cne.23774</a>
  apa: Javdani, F., Holló, K., Hegedűs, K., Kis, G., Hegyi, Z., Dócs, K., … Antal,
    M. (2015). Differential expression patterns of K+Cl- cotransporter 2 in neurons
    within the superficial spinal dorsal horn of rats. <i>Journal of Comparative Neurology</i>.
    Wiley-Blackwell. <a href="https://doi.org/10.1002/cne.23774">https://doi.org/10.1002/cne.23774</a>
  chicago: Javdani, Fariba, Krisztina Holló, Krisztina Hegedűs, Gréta Kis, Zoltán
    Hegyi, Klaudia Dócs, Yu Kasugai, Yugo Fukazawa, Ryuichi Shigemoto, and Miklós
    Antal. “Differential Expression Patterns of K+Cl- Cotransporter 2 in Neurons within
    the Superficial Spinal Dorsal Horn of Rats.” <i>Journal of Comparative Neurology</i>.
    Wiley-Blackwell, 2015. <a href="https://doi.org/10.1002/cne.23774">https://doi.org/10.1002/cne.23774</a>.
  ieee: F. Javdani <i>et al.</i>, “Differential expression patterns of K+Cl- cotransporter
    2 in neurons within the superficial spinal dorsal horn of rats,” <i>Journal of
    Comparative Neurology</i>, vol. 523, no. 13. Wiley-Blackwell, pp. 1967–1983, 2015.
  ista: Javdani F, Holló K, Hegedűs K, Kis G, Hegyi Z, Dócs K, Kasugai Y, Fukazawa
    Y, Shigemoto R, Antal M. 2015. Differential expression patterns of K+Cl- cotransporter
    2 in neurons within the superficial spinal dorsal horn of rats. Journal of Comparative
    Neurology. 523(13), 1967–1983.
  mla: Javdani, Fariba, et al. “Differential Expression Patterns of K+Cl- Cotransporter
    2 in Neurons within the Superficial Spinal Dorsal Horn of Rats.” <i>Journal of
    Comparative Neurology</i>, vol. 523, no. 13, Wiley-Blackwell, 2015, pp. 1967–83,
    doi:<a href="https://doi.org/10.1002/cne.23774">10.1002/cne.23774</a>.
  short: F. Javdani, K. Holló, K. Hegedűs, G. Kis, Z. Hegyi, K. Dócs, Y. Kasugai,
    Y. Fukazawa, R. Shigemoto, M. Antal, Journal of Comparative Neurology 523 (2015)
    1967–1983.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-09-01T00:00:00Z
date_updated: 2025-09-23T08:26:48Z
day: '01'
department:
- _id: RySh
doi: 10.1002/cne.23774
external_id:
  isi:
  - '000358228700006'
fulldoi: https://doi.org/10.1002/cne.23774
intvolume: '       523'
isi: 1
issue: '13'
language:
- iso: eng
month: '09'
oa_version: None
page: 1967 - 1983
publication: Journal of Comparative Neurology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5614'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differential expression patterns of K+Cl- cotransporter 2 in neurons within
  the superficial spinal dorsal horn of rats
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 523
year: '2015'
...