---
_id: '1558'
abstract:
- lang: eng
text: CyclophilinAis a conserved peptidyl-prolyl cis-trans isomerase (PPIase) best
known as the cellular receptor of the immunosuppressant cyclosporine A. Despite
significant effort, evidence of developmental functions of cyclophilin A in non-plant
systems has remained obscure. Mutations in a tomato (Solanum lycopersicum) cyclophilin
A ortholog, DIAGEOTROPICA (DGT), have been shown to abolish the organogenesis
of lateral roots; however, a mechanistic explanation of the phenotype is lacking.
Here, we show that the dgt mutant lacks auxin maxima relevant to priming and specification
of lateral root founder cells. DGT is expressed in shoot and root, and localizes
to both the nucleus and cytoplasm during lateral root organogenesis. Mutation
of ENTIRE/ IAA9, a member of the auxin-responsive Aux/IAA protein family of transcriptional
repressors, partially restores the inability of dgt to initiate lateral root primordia
but not the primordia outgrowth. By comparison, grafting of a wild-type scion
restores the process of lateral root formation, consistent with participation
of a mobile signal. Antibodies do not detect movement of the DGT protein into
the dgt rootstock; however, experiments with radiolabeled auxin and an auxin-specific
microelectrode demonstrate abnormal auxin fluxes. Functional studies of DGT in
heterologous yeast and tobacco-leaf auxin-transport systems demonstrate that DGT
negatively regulates PIN-FORMED (PIN) auxin efflux transporters by affecting their
plasma membrane localization. Studies in tomato support complex effects of the
dgt mutation on PIN expression level, expression domain and plasma membrane localization.
Our data demonstrate that DGT regulates auxin transport in lateral root formation.
author:
- first_name: Maria
full_name: Ivanchenko, Maria
last_name: Ivanchenko
- first_name: Jinsheng
full_name: Zhu, Jinsheng
last_name: Zhu
- first_name: Bangjun
full_name: Wang, Bangjun
last_name: Wang
- first_name: Eva
full_name: Medvecka, Eva
id: 298814E2-F248-11E8-B48F-1D18A9856A87
last_name: Medvecka
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Molly
full_name: Megraw, Molly
last_name: Megraw
- first_name: Sergei
full_name: Filichkin, Sergei
last_name: Filichkin
- first_name: Joseph
full_name: Dubrovsky, Joseph
last_name: Dubrovsky
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Ivanchenko M, Zhu J, Wang B, et al. The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation. Development.
2015;142(4):712-721. doi:10.1242/dev.113225
apa: Ivanchenko, M., Zhu, J., Wang, B., Medvecka, E., Du, Y., Azzarello, E., … Geisler,
M. (2015). The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both
root and shoot to control lateral root formation. Development. Company
of Biologists. https://doi.org/10.1242/dev.113225
chicago: Ivanchenko, Maria, Jinsheng Zhu, Bangjun Wang, Eva Medvecka, Yunlong Du,
Elisa Azzarello, Stefano Mancuso, et al. “The Cyclophilin a DIAGEOTROPICA Gene
Affects Auxin Transport in Both Root and Shoot to Control Lateral Root Formation.”
Development. Company of Biologists, 2015. https://doi.org/10.1242/dev.113225.
ieee: M. Ivanchenko et al., “The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation,” Development,
vol. 142, no. 4. Company of Biologists, pp. 712–721, 2015.
ista: Ivanchenko M, Zhu J, Wang B, Medvecka E, Du Y, Azzarello E, Mancuso S, Megraw
M, Filichkin S, Dubrovsky J, Friml J, Geisler M. 2015. The cyclophilin a DIAGEOTROPICA
gene affects auxin transport in both root and shoot to control lateral root formation.
Development. 142(4), 712–721.
mla: Ivanchenko, Maria, et al. “The Cyclophilin a DIAGEOTROPICA Gene Affects Auxin
Transport in Both Root and Shoot to Control Lateral Root Formation.” Development,
vol. 142, no. 4, Company of Biologists, 2015, pp. 712–21, doi:10.1242/dev.113225.
short: M. Ivanchenko, J. Zhu, B. Wang, E. Medvecka, Y. Du, E. Azzarello, S. Mancuso,
M. Megraw, S. Filichkin, J. Dubrovsky, J. Friml, M. Geisler, Development 142 (2015)
712–721.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.113225
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 712 - 721
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5613'
quality_controlled: '1'
scopus_import: 1
status: public
title: The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both root and
shoot to control lateral root formation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...