---
_id: '1562'
abstract:
- lang: eng
  text: The plant hormone auxin is a key regulator of plant growth and development.
    Auxin levels are sensed and interpreted by distinct receptor systems that activate
    a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has
    been identified based on its ability to bind auxin with high affinity is a prime
    candidate for the extracellular receptor responsible for mediating a range of
    auxin effects, in particular, the fast non-transcriptional ones. Contradictory
    genetic studies suggested prominent or no importance of ABP1 in many developmental
    processes. However, how crucial the role of auxin binding to ABP1 is for its functions
    has not been addressed. Here, we show that the auxin-binding pocket of ABP1 is
    essential for its gain-of-function cellular and developmental roles. In total,
    16 different abp1 mutants were prepared that possessed substitutions in the metal
    core or in the hydrophobic amino acids of the auxin-binding pocket as well as
    neutral mutations. Their analysis revealed that an intact auxin-binding pocket
    is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling
    pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association
    with membranes for endocytosis regulation. In planta analyses demonstrated the
    importance of the auxin binding pocket for all known ABP1-mediated postembryonic
    developmental processes, including morphology of leaf epidermal cells, root growth
    and root meristem activity, and vascular tissue differentiation. Taken together,
    these findings suggest that auxin binding to ABP1 is central to its function,
    supporting the role of ABP1 as auxin receptor.
acknowledgement: This work was supported by ERC Independent Research grant (ERC-2011-StG-
  20101109-PSDP to JF); the European Social Fund and the state budget of the Czech
  Republic [the project ‘Employment of Newly Graduated Doctors of Science for Scientific
  Excellence’ (CZ.1.07/2.3.00/30.0009) to TN]; the Czech Science Foundation (GACR)
  [project 13-40637S to JF].
article_processing_charge: No
article_type: original
author:
- first_name: Peter
  full_name: Grones, Peter
  id: 399876EC-F248-11E8-B48F-1D18A9856A87
  last_name: Grones
- first_name: Xu
  full_name: Chen, Xu
  id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
  last_name: Chen
- first_name: Sibu
  full_name: Simon, Sibu
  id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
  last_name: Simon
  orcid: 0000-0002-1998-6741
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Riet
  full_name: De Rycke, Riet
  last_name: De Rycke
- first_name: Tomasz
  full_name: Nodzyński, Tomasz
  last_name: Nodzyński
- first_name: Eva
  full_name: Zažímalová, Eva
  last_name: Zažímalová
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Grones P, Chen X, Simon S, et al. Auxin-binding pocket of ABP1 is crucial for
    its gain-of-function cellular and developmental roles. <i>Journal of Experimental
    Botany</i>. 2015;66(16):5055-5065. doi:<a href="https://doi.org/10.1093/jxb/erv177">10.1093/jxb/erv177</a>
  apa: Grones, P., Chen, X., Simon, S., Kaufmann, W., De Rycke, R., Nodzyński, T.,
    … Friml, J. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function
    cellular and developmental roles. <i>Journal of Experimental Botany</i>. Oxford
    University Press. <a href="https://doi.org/10.1093/jxb/erv177">https://doi.org/10.1093/jxb/erv177</a>
  chicago: Grones, Peter, Xu Chen, Sibu Simon, Walter Kaufmann, Riet De Rycke, Tomasz
    Nodzyński, Eva Zažímalová, and Jiří Friml. “Auxin-Binding Pocket of ABP1 Is Crucial
    for Its Gain-of-Function Cellular and Developmental Roles.” <i>Journal of Experimental
    Botany</i>. Oxford University Press, 2015. <a href="https://doi.org/10.1093/jxb/erv177">https://doi.org/10.1093/jxb/erv177</a>.
  ieee: P. Grones <i>et al.</i>, “Auxin-binding pocket of ABP1 is crucial for its
    gain-of-function cellular and developmental roles,” <i>Journal of Experimental
    Botany</i>, vol. 66, no. 16. Oxford University Press, pp. 5055–5065, 2015.
  ista: Grones P, Chen X, Simon S, Kaufmann W, De Rycke R, Nodzyński T, Zažímalová
    E, Friml J. 2015. Auxin-binding pocket of ABP1 is crucial for its gain-of-function
    cellular and developmental roles. Journal of Experimental Botany. 66(16), 5055–5065.
  mla: Grones, Peter, et al. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function
    Cellular and Developmental Roles.” <i>Journal of Experimental Botany</i>, vol.
    66, no. 16, Oxford University Press, 2015, pp. 5055–65, doi:<a href="https://doi.org/10.1093/jxb/erv177">10.1093/jxb/erv177</a>.
  short: P. Grones, X. Chen, S. Simon, W. Kaufmann, R. De Rycke, T. Nodzyński, E.
    Zažímalová, J. Friml, Journal of Experimental Botany 66 (2015) 5055–5065.
corr_author: '1'
date_created: 2018-12-11T11:52:44Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2025-09-23T07:59:07Z
day: '01'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1093/jxb/erv177
ec_funded: 1
external_id:
  isi:
  - '000359688300017'
intvolume: '        66'
isi: 1
issue: '16'
language:
- iso: eng
month: '08'
oa_version: None
page: 5055 - 5065
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '5609'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and
  developmental roles
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 66
year: '2015'
...