---
res:
  bibo_abstract:
  - Leptin is an adipokine produced by the adipose tissue regulating body weight through
    its appetite-suppressing effect. Besides being expressed in the hypothalamus and
    hippocampus, leptin receptors (ObRs) are also present in chromaffin cells of the
    adrenal medulla. In the present study, we report the effect of leptin on mouse
    chromaffin cell (MCC) functionality, focusing on cell excitability and catecholamine
    secretion. Acute application of leptin (1 nm) on spontaneously firing MCCs caused
    a slowly developing membrane hyperpolarization followed by complete blockade of
    action potential (AP) firing. This inhibitory effect at rest was abolished by
    the BK channel blocker paxilline (1 μm), suggesting the involvement of BK potassium
    channels. Single-channel recordings in 'perforated microvesicles' confirmed that
    leptin increased BK channel open probability without altering its unitary conductance.
    BK channel up-regulation was associated with the phosphoinositide 3-kinase (PI3K)
    signalling cascade because the PI3K specific inhibitor wortmannin (100 nm) fully
    prevented BK current increase. We also tested the effect of leptin on evoked AP
    firing and Ca2+-driven exocytosis. Although leptin preserves well-adapted AP trains
    of lower frequency, APs are broader and depolarization-evoked exocytosis is increased
    as a result of the larger size of the ready-releasable pool and higher frequency
    of vesicle release. The kinetics and quantal size of single secretory events remained
    unaltered. Leptin had no effect on firing and secretion in db-/db- mice lacking
    the ObR gene, confirming its specificity. In conclusion, leptin exhibits a dual
    action on MCC activity. It dampens AP firing at rest but preserves AP firing and
    increases catecholamine secretion during sustained stimulation, highlighting the
    importance of the adipo-adrenal axis in the leptin-mediated increase of sympathetic
    tone and catecholamine release.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Daniela
      foaf_name: Gavello, Daniela
      foaf_surname: Gavello
  - foaf_Person:
      foaf_givenName: David H
      foaf_name: Vandael, David H
      foaf_surname: Vandael
      foaf_workInfoHomepage: http://www.librecat.org/personId=3AE48E0A-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7577-1676
  - foaf_Person:
      foaf_givenName: Sara
      foaf_name: Gosso, Sara
      foaf_surname: Gosso
  - foaf_Person:
      foaf_givenName: Emilio
      foaf_name: Carbone, Emilio
      foaf_surname: Carbone
  - foaf_Person:
      foaf_givenName: Valentina
      foaf_name: Carabelli, Valentina
      foaf_surname: Carabelli
  bibo_doi: 10.1113/JP271078
  bibo_issue: '22'
  bibo_volume: 593
  dct_date: 2015^xs_gYear
  dct_identifier:
  - UT:000365267700003
  dct_language: eng
  dct_publisher: Wiley-Blackwell@
  dct_title: Dual action of leptin on rest-firing and stimulated catecholamine release
    via phosphoinositide 3-kinase-riven BK channel up-regulation in mouse chromaffin
    cells@
  fabio_hasPubmedId: '26282459'
...