--- res: bibo_abstract: - Leptin is an adipokine produced by the adipose tissue regulating body weight through its appetite-suppressing effect. Besides being expressed in the hypothalamus and hippocampus, leptin receptors (ObRs) are also present in chromaffin cells of the adrenal medulla. In the present study, we report the effect of leptin on mouse chromaffin cell (MCC) functionality, focusing on cell excitability and catecholamine secretion. Acute application of leptin (1 nm) on spontaneously firing MCCs caused a slowly developing membrane hyperpolarization followed by complete blockade of action potential (AP) firing. This inhibitory effect at rest was abolished by the BK channel blocker paxilline (1 μm), suggesting the involvement of BK potassium channels. Single-channel recordings in 'perforated microvesicles' confirmed that leptin increased BK channel open probability without altering its unitary conductance. BK channel up-regulation was associated with the phosphoinositide 3-kinase (PI3K) signalling cascade because the PI3K specific inhibitor wortmannin (100 nm) fully prevented BK current increase. We also tested the effect of leptin on evoked AP firing and Ca2+-driven exocytosis. Although leptin preserves well-adapted AP trains of lower frequency, APs are broader and depolarization-evoked exocytosis is increased as a result of the larger size of the ready-releasable pool and higher frequency of vesicle release. The kinetics and quantal size of single secretory events remained unaltered. Leptin had no effect on firing and secretion in db-/db- mice lacking the ObR gene, confirming its specificity. In conclusion, leptin exhibits a dual action on MCC activity. It dampens AP firing at rest but preserves AP firing and increases catecholamine secretion during sustained stimulation, highlighting the importance of the adipo-adrenal axis in the leptin-mediated increase of sympathetic tone and catecholamine release.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Daniela foaf_name: Gavello, Daniela foaf_surname: Gavello - foaf_Person: foaf_givenName: David H foaf_name: Vandael, David H foaf_surname: Vandael foaf_workInfoHomepage: http://www.librecat.org/personId=3AE48E0A-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-7577-1676 - foaf_Person: foaf_givenName: Sara foaf_name: Gosso, Sara foaf_surname: Gosso - foaf_Person: foaf_givenName: Emilio foaf_name: Carbone, Emilio foaf_surname: Carbone - foaf_Person: foaf_givenName: Valentina foaf_name: Carabelli, Valentina foaf_surname: Carabelli bibo_doi: 10.1113/JP271078 bibo_issue: '22' bibo_volume: 593 dct_date: 2015^xs_gYear dct_language: eng dct_publisher: Wiley-Blackwell@ dct_title: Dual action of leptin on rest-firing and stimulated catecholamine release via phosphoinositide 3-kinase-riven BK channel up-regulation in mouse chromaffin cells@ fabio_hasPubmedId: '26282459' ...