---
_id: '1597'
abstract:
- lang: eng
  text: Chemokines are the main guidance cues directing leukocyte migration. Opposed
    to early assumptions, chemokines do not necessarily act as soluble cues but are
    often immobilized within tissues, e.g., dendritic cell migration toward lymphatic
    vessels is guided by a haptotactic gradient of the chemokine CCL21. Controlled
    assay systems to quantitatively study haptotaxis in vitro are still missing. In
    this chapter, we describe an in vitro haptotaxis assay optimized for the unique
    properties of dendritic cells. The chemokine CCL21 is immobilized in a bioactive
    state, using laser-assisted protein adsorption by photobleaching. The cells follow
    this immobilized CCL21 gradient in a haptotaxis chamber, which provides three
    dimensionally confined migration conditions.
acknowledged_ssus:
- _id: Bio
acknowledgement: This work was supported by the Boehringer Ingelheim Fonds, the European
  Research Council (ERC StG 281556), and a START Award of the Austrian Science Foundation
  (FWF). We thank Robert Hauschild, Anne Reversat, and Jack Merrin for valuable input
  and the Imaging Facility of IST Austria for excellent support.
article_processing_charge: No
article_type: original
author:
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: Schwarz J, Sixt MK. Quantitative analysis of dendritic cell haptotaxis. <i>Methods
    in Enzymology</i>. 2016;570:567-581. doi:<a href="https://doi.org/10.1016/bs.mie.2015.11.004">10.1016/bs.mie.2015.11.004</a>
  apa: Schwarz, J., &#38; Sixt, M. K. (2016). Quantitative analysis of dendritic cell
    haptotaxis. <i>Methods in Enzymology</i>. Elsevier. <a href="https://doi.org/10.1016/bs.mie.2015.11.004">https://doi.org/10.1016/bs.mie.2015.11.004</a>
  chicago: Schwarz, Jan, and Michael K Sixt. “Quantitative Analysis of Dendritic Cell
    Haptotaxis.” <i>Methods in Enzymology</i>. Elsevier, 2016. <a href="https://doi.org/10.1016/bs.mie.2015.11.004">https://doi.org/10.1016/bs.mie.2015.11.004</a>.
  ieee: J. Schwarz and M. K. Sixt, “Quantitative analysis of dendritic cell haptotaxis,”
    <i>Methods in Enzymology</i>, vol. 570. Elsevier, pp. 567–581, 2016.
  ista: Schwarz J, Sixt MK. 2016. Quantitative analysis of dendritic cell haptotaxis.
    Methods in Enzymology. 570, 567–581.
  mla: Schwarz, Jan, and Michael K. Sixt. “Quantitative Analysis of Dendritic Cell
    Haptotaxis.” <i>Methods in Enzymology</i>, vol. 570, Elsevier, 2016, pp. 567–81,
    doi:<a href="https://doi.org/10.1016/bs.mie.2015.11.004">10.1016/bs.mie.2015.11.004</a>.
  short: J. Schwarz, M.K. Sixt, Methods in Enzymology 570 (2016) 567–581.
corr_author: '1'
date_created: 2018-12-11T11:52:56Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2025-09-18T11:02:13Z
day: '01'
department:
- _id: MiSi
doi: 10.1016/bs.mie.2015.11.004
ec_funded: 1
external_id:
  isi:
  - '000375648700025'
  pmid:
  - '26921962'
intvolume: '       570'
isi: 1
language:
- iso: eng
month: '01'
oa_version: None
page: 567 - 581
pmid: 1
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '281556'
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: Y 564-B12
  name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Methods in Enzymology
publication_status: published
publisher: Elsevier
publist_id: '5573'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantitative analysis of dendritic cell haptotaxis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 570
year: '2016'
...