article published yes Petter Hammar author Andreas Angermayr author 4677C796-F248-11E8-B48F-1D18A9856A870000-0001-8619-2223 Staffan Sjostrom author Josefin Van Der Meer author Klaas Hellingwerf author Elton Hudson author Hakaan Joensson author ToBo department Background Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible. Results We present a method for high-throughput, single-cell analysis and sorting of genetically engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for l-lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive l-lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells. Conclusions The workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology. https://research-explorer.ista.ac.at/download/1623/4796/IST-2016-467-v1+1_s13068-015-0380-2.pdf application/pdfno https://creativecommons.org/licenses/by/4.0/ BioMed Central2015 eng 00036578400000910.1186/s13068-015-0380-2 81 P. Hammar <i>et al.</i>, “Single-cell screening of photosynthetic growth and lactate production by cyanobacteria,” <i>Biotechnology for Biofuels</i>, vol. 8, no. 1. BioMed Central, 2015. Hammar, P., Angermayr, A., Sjostrom, S., Van Der Meer, J., Hellingwerf, K., Hudson, E., &#38; Joensson, H. (2015). Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. <i>Biotechnology for Biofuels</i>. BioMed Central. <a href="https://doi.org/10.1186/s13068-015-0380-2">https://doi.org/10.1186/s13068-015-0380-2</a> Hammar, Petter, et al. “Single-Cell Screening of Photosynthetic Growth and Lactate Production by Cyanobacteria.” <i>Biotechnology for Biofuels</i>, vol. 8, no. 1, 193, BioMed Central, 2015, doi:<a href="https://doi.org/10.1186/s13068-015-0380-2">10.1186/s13068-015-0380-2</a>. Hammar P, Angermayr A, Sjostrom S, Van Der Meer J, Hellingwerf K, Hudson E, Joensson H. 2015. Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. Biotechnology for Biofuels. 8(1), 193. P. Hammar, A. Angermayr, S. Sjostrom, J. Van Der Meer, K. Hellingwerf, E. Hudson, H. Joensson, Biotechnology for Biofuels 8 (2015). Hammar P, Angermayr A, Sjostrom S, et al. Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. <i>Biotechnology for Biofuels</i>. 2015;8(1). doi:<a href="https://doi.org/10.1186/s13068-015-0380-2">10.1186/s13068-015-0380-2</a> Hammar, Petter, Andreas Angermayr, Staffan Sjostrom, Josefin Van Der Meer, Klaas Hellingwerf, Elton Hudson, and Hakaan Joensson. “Single-Cell Screening of Photosynthetic Growth and Lactate Production by Cyanobacteria.” <i>Biotechnology for Biofuels</i>. BioMed Central, 2015. <a href="https://doi.org/10.1186/s13068-015-0380-2">https://doi.org/10.1186/s13068-015-0380-2</a>. 16232018-12-11T11:53:05Z2025-09-23T10:45:43Z