---
_id: '1623'
abstract:
- lang: eng
  text: "Background\r\nPhotosynthetic cyanobacteria are attractive for a range of
    biotechnological applications including biofuel production. However, due to slow
    growth, screening of mutant libraries using microtiter plates is not feasible.\r\nResults\r\nWe
    present a method for high-throughput, single-cell analysis and sorting of genetically
    engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic
    device is used to encapsulate single cells in picoliter droplets, assay the droplets
    for l-lactate production, and sort strains with high productivity. We demonstrate
    the separation of low- and high-producing reference strains, as well as enrichment
    of a more productive l-lactate-synthesizing population after UV-induced mutagenesis.
    The droplet platform also revealed population heterogeneity in photosynthetic
    growth and lactate production, as well as the presence of metabolically stalled
    cells.\r\nConclusions\r\nThe workflow will facilitate metabolic engineering and
    directed evolution studies and will be useful in studies of cyanobacteria biochemistry
    and physiology.\r\n"
article_number: '193'
article_processing_charge: No
author:
- first_name: Petter
  full_name: Hammar, Petter
  last_name: Hammar
- first_name: Andreas
  full_name: Angermayr, Andreas
  id: 4677C796-F248-11E8-B48F-1D18A9856A87
  last_name: Angermayr
  orcid: 0000-0001-8619-2223
- first_name: Staffan
  full_name: Sjostrom, Staffan
  last_name: Sjostrom
- first_name: Josefin
  full_name: Van Der Meer, Josefin
  last_name: Van Der Meer
- first_name: Klaas
  full_name: Hellingwerf, Klaas
  last_name: Hellingwerf
- first_name: Elton
  full_name: Hudson, Elton
  last_name: Hudson
- first_name: Hakaan
  full_name: Joensson, Hakaan
  last_name: Joensson
citation:
  ama: Hammar P, Angermayr A, Sjostrom S, et al. Single-cell screening of photosynthetic
    growth and lactate production by cyanobacteria. <i>Biotechnology for Biofuels</i>.
    2015;8(1). doi:<a href="https://doi.org/10.1186/s13068-015-0380-2">10.1186/s13068-015-0380-2</a>
  apa: Hammar, P., Angermayr, A., Sjostrom, S., Van Der Meer, J., Hellingwerf, K.,
    Hudson, E., &#38; Joensson, H. (2015). Single-cell screening of photosynthetic
    growth and lactate production by cyanobacteria. <i>Biotechnology for Biofuels</i>.
    BioMed Central. <a href="https://doi.org/10.1186/s13068-015-0380-2">https://doi.org/10.1186/s13068-015-0380-2</a>
  chicago: Hammar, Petter, Andreas Angermayr, Staffan Sjostrom, Josefin Van Der Meer,
    Klaas Hellingwerf, Elton Hudson, and Hakaan Joensson. “Single-Cell Screening of
    Photosynthetic Growth and Lactate Production by Cyanobacteria.” <i>Biotechnology
    for Biofuels</i>. BioMed Central, 2015. <a href="https://doi.org/10.1186/s13068-015-0380-2">https://doi.org/10.1186/s13068-015-0380-2</a>.
  ieee: P. Hammar <i>et al.</i>, “Single-cell screening of photosynthetic growth and
    lactate production by cyanobacteria,” <i>Biotechnology for Biofuels</i>, vol.
    8, no. 1. BioMed Central, 2015.
  ista: Hammar P, Angermayr A, Sjostrom S, Van Der Meer J, Hellingwerf K, Hudson E,
    Joensson H. 2015. Single-cell screening of photosynthetic growth and lactate production
    by cyanobacteria. Biotechnology for Biofuels. 8(1), 193.
  mla: Hammar, Petter, et al. “Single-Cell Screening of Photosynthetic Growth and
    Lactate Production by Cyanobacteria.” <i>Biotechnology for Biofuels</i>, vol.
    8, no. 1, 193, BioMed Central, 2015, doi:<a href="https://doi.org/10.1186/s13068-015-0380-2">10.1186/s13068-015-0380-2</a>.
  short: P. Hammar, A. Angermayr, S. Sjostrom, J. Van Der Meer, K. Hellingwerf, E.
    Hudson, H. Joensson, Biotechnology for Biofuels 8 (2015).
date_created: 2018-12-11T11:53:05Z
date_published: 2015-11-25T00:00:00Z
date_updated: 2025-09-23T10:45:43Z
day: '25'
ddc:
- '570'
department:
- _id: ToBo
doi: 10.1186/s13068-015-0380-2
external_id:
  isi:
  - '000365784000009'
file:
- access_level: open_access
  checksum: 172b0b6f4eb2e5c22b7cec1d57dc0107
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:10:11Z
  date_updated: 2020-07-14T12:45:07Z
  file_id: '4796'
  file_name: IST-2016-467-v1+1_s13068-015-0380-2.pdf
  file_size: 2914089
  relation: main_file
file_date_updated: 2020-07-14T12:45:07Z
has_accepted_license: '1'
intvolume: '         8'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
publication: Biotechnology for Biofuels
publication_status: published
publisher: BioMed Central
publist_id: '5537'
pubrep_id: '467'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single-cell screening of photosynthetic growth and lactate production by cyanobacteria
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 8
year: '2015'
...