Cytokinin response factors regulate PIN-FORMED auxin transporters Šimášková, Mária O'Brien, José Khan-Djamei, Mamoona Van Noorden, Giel Ötvös, Krisztina ; https://orcid.org/0000-0002-5503-4983 Vieten, Anne De Clercq, Inge Van Haperen, Johanna Cuesta, Candela ; https://orcid.org/0000-0003-1923-2410 Hoyerová, Klára Vanneste, Steffen Marhavy, Peter ; https://orcid.org/0000-0001-5227-5741 Wabnik, Krzysztof T ; https://orcid.org/0000-0001-7263-0560 Van Breusegem, Frank Nowack, Moritz Murphy, Angus Friml, Jiřĺ ; https://orcid.org/0000-0002-8302-7596 Weijers, Dolf Beeckman, Tom Benková, Eva ; https://orcid.org/0000-0002-8510-9739 ddc:580 Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development. Nature Publishing Group 2015 info:eu-repo/semantics/article doc-type:article article http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/1640 https://research-explorer.ista.ac.at/download/1640/5358 Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate PIN-FORMED auxin transporters. <i>Nature Communications</i>. 2015;6. doi:<a href="https://doi.org/10.1038/ncomms9717">10.1038/ncomms9717</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms9717 info:eu-repo/semantics/altIdentifier/wos/000366289800001 info:eu-repo/semantics/openAccess