---
OA_place: repository
OA_type: green
_id: '1663'
abstract:
- lang: eng
  text: CREB-binding protein (CBP) and p300 are transcriptional coactivators involved
    in numerous biological processes that affect cell growth, transformation, differentiation,
    and development. In this study, we provide evidence of the involvement of homeodomain-interacting
    protein kinase 2 (HIPK2) in the regulation of CBP activity. We show that HIPK2
    interacts with and phosphorylates several regions of CBP. We demonstrate that
    serines 2361, 2363, 2371, 2376, and 2381 are responsible for the HIPK2-induced
    mobility shift of CBP C-terminal activation domain. Moreover, we show that HIPK2
    strongly potentiates the transcriptional activity of CBP. However, our data suggest
    that HIPK2 activates CBP mainly by counteracting the repressive action of cell
    cycle regulatory domain 1 (CRD1), located between amino acids 977 and 1076, independently
    of CBP phosphorylation. Our findings thus highlight a complex regulation of CBP
    activity by HIPK2, which might be relevant for the control of specific sets of
    target genes involved in cellular proliferation, differentiation and apoptosis.
acknowledgement: 'We thank Silvia Soddu for the pCMV-HIPK2-FLAG expression vector.
  This work was supported by Swiss National Science Foundation Grants 3100A0-105773,
  3100A0-120699, and 31003A-135692 to J-R. C., and partly by an IST Fellowship awarded
  to K. A. K. (Project: P IST EU01 under REA grant agreement n ° 291734).'
article_processing_charge: No
author:
- first_name: Krisztián
  full_name: Kovács, Krisztián
  id: 2AB5821E-F248-11E8-B48F-1D18A9856A87
  last_name: Kovács
- first_name: Myriam
  full_name: Steinmann, Myriam
  last_name: Steinmann
- first_name: Olivier
  full_name: Halfon, Olivier
  last_name: Halfon
- first_name: Pierre
  full_name: Magistretti, Pierre
  last_name: Magistretti
- first_name: Jean
  full_name: Cardinaux, Jean
  last_name: Cardinaux
citation:
  ama: Kovács K, Steinmann M, Halfon O, Magistretti P, Cardinaux J. Complex regulation
    of CREB-binding protein by homeodomain-interacting protein kinase 2. <i>Cellular
    Signalling</i>. 2015;27(11):2252-2260. doi:<a href="https://doi.org/10.1016/j.cellsig.2015.08.001">10.1016/j.cellsig.2015.08.001</a>
  apa: Kovács, K., Steinmann, M., Halfon, O., Magistretti, P., &#38; Cardinaux, J.
    (2015). Complex regulation of CREB-binding protein by homeodomain-interacting
    protein kinase 2. <i>Cellular Signalling</i>. Elsevier. <a href="https://doi.org/10.1016/j.cellsig.2015.08.001">https://doi.org/10.1016/j.cellsig.2015.08.001</a>
  chicago: Kovács, Krisztián, Myriam Steinmann, Olivier Halfon, Pierre Magistretti,
    and Jean Cardinaux. “Complex Regulation of CREB-Binding Protein by Homeodomain-Interacting
    Protein Kinase 2.” <i>Cellular Signalling</i>. Elsevier, 2015. <a href="https://doi.org/10.1016/j.cellsig.2015.08.001">https://doi.org/10.1016/j.cellsig.2015.08.001</a>.
  ieee: K. Kovács, M. Steinmann, O. Halfon, P. Magistretti, and J. Cardinaux, “Complex
    regulation of CREB-binding protein by homeodomain-interacting protein kinase 2,”
    <i>Cellular Signalling</i>, vol. 27, no. 11. Elsevier, pp. 2252–2260, 2015.
  ista: Kovács K, Steinmann M, Halfon O, Magistretti P, Cardinaux J. 2015. Complex
    regulation of CREB-binding protein by homeodomain-interacting protein kinase 2.
    Cellular Signalling. 27(11), 2252–2260.
  mla: Kovács, Krisztián, et al. “Complex Regulation of CREB-Binding Protein by Homeodomain-Interacting
    Protein Kinase 2.” <i>Cellular Signalling</i>, vol. 27, no. 11, Elsevier, 2015,
    pp. 2252–60, doi:<a href="https://doi.org/10.1016/j.cellsig.2015.08.001">10.1016/j.cellsig.2015.08.001</a>.
  short: K. Kovács, M. Steinmann, O. Halfon, P. Magistretti, J. Cardinaux, Cellular
    Signalling 27 (2015) 2252–2260.
corr_author: '1'
date_created: 2018-12-11T11:53:20Z
date_published: 2015-11-01T00:00:00Z
date_updated: 2025-09-23T08:19:10Z
day: '01'
ddc:
- '570'
department:
- _id: JoCs
doi: 10.1016/j.cellsig.2015.08.001
ec_funded: 1
external_id:
  isi:
  - '000361777500012'
  pmid:
  - '26247811'
file:
- access_level: open_access
  checksum: 4ee690b6444b7a43523237f0941457d1
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:18:03Z
  date_updated: 2025-06-26T06:47:23Z
  file_id: '5321'
  file_name: IST-2016-578-v1+1_CLS-D-15-00072R1_.pdf
  file_size: 1735337
  relation: main_file
file_date_updated: 2025-06-26T06:47:23Z
has_accepted_license: '1'
intvolume: '        27'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 2252 - 2260
pmid: 1
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publication: Cellular Signalling
publication_status: published
publisher: Elsevier
publist_id: '5487'
pubrep_id: '578'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Complex regulation of CREB-binding protein by homeodomain-interacting protein
  kinase 2
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 27
year: '2015'
...