[{"date_created":"2018-12-11T11:53:25Z","date_published":"2015-10-12T00:00:00Z","page":"952 - 954","uri_base":"https://research-explorer.ista.ac.at","dc":{"date":["2015"],"publisher":["Nature Publishing Group"],"type":["info:eu-repo/semantics/article","doc-type:article","text","http://purl.org/coar/resource_type/c_6501"],"source":["Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 2015;11(12):952-954. doi:10.1038/nchembio.1933"],"relation":["info:eu-repo/semantics/altIdentifier/doi/10.1038/nchembio.1933","info:eu-repo/grantAgreement/EC/FP7/303564","info:eu-repo/grantAgreement/FWF//RGY0084/2012","info:eu-repo/grantAgreement/FWF//W1232-B24"],"description":["High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes."],"identifier":["https://research-explorer.ista.ac.at/record/1678","https://research-explorer.ista.ac.at/download/1678/4842"],"title":["Light-assisted small-molecule screening against protein kinases"],"language":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"creator":["Inglés Prieto, Álvaro","Gschaider-Reichhart, Eva","Muellner, Markus","Nowak, Matthias","Nijman, Sebastian","Grusch, Michael","Janovjak, Harald L"],"subject":["ddc:571"]},"publication":"Nature Chemical Biology","day":"12","has_accepted_license":"1","oa":1,"quality_controlled":"1","acknowledgement":"This work was supported by grants from the European Union Seventh Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech fellowship (3580812 Austrian Research Promotion Agency).","publist_id":"5471","author":[{"id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","first_name":"Álvaro","last_name":"Inglés Prieto","orcid":"0000-0002-5409-8571"},{"first_name":"Eva","id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7218-7738","last_name":"Gschaider-Reichhart"},{"first_name":"Markus","last_name":"Muellner"},{"id":"30845DAA-F248-11E8-B48F-1D18A9856A87","first_name":"Matthias","last_name":"Nowak"},{"first_name":"Sebastian","last_name":"Nijman"},{"last_name":"Grusch","first_name":"Michael"},{"first_name":"Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","orcid":"0000-0002-8023-9315"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","dini_type":"doc-type:article","citation":{"chicago":"Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted Small-Molecule Screening against Protein Kinases.” Nature Chemical Biology. Nature Publishing Group, 2015. https://doi.org/10.1038/nchembio.1933.","ista":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 11(12), 952–954.","mla":"Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against Protein Kinases.” Nature Chemical Biology, vol. 11, no. 12, Nature Publishing Group, 2015, pp. 952–54, doi:10.1038/nchembio.1933.","apa":"Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman, S., Grusch, M., & Janovjak, H. L. (2015). Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. Nature Publishing Group. https://doi.org/10.1038/nchembio.1933","ieee":"Á. Inglés Prieto et al., “Light-assisted small-molecule screening against protein kinases,” Nature Chemical Biology, vol. 11, no. 12. Nature Publishing Group, pp. 952–954, 2015.","short":"Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman, M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954."},"project":[{"name":"Microbial Ion Channels for Synthetic Neurobiology","_id":"25548C20-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)","_id":"255BFFFA-B435-11E9-9278-68D0E5697425"},{"name":"Molecular Drug Targets","call_identifier":"FWF","_id":"255A6082-B435-11E9-9278-68D0E5697425"}],"ec_funded":1,"volume":11,"issue":"12","related_material":{"record":[{"id":"418","status":"public","relation":"dissertation_contains"}]},"language":[{}],"file":[{"content_type":"application/pdf","access_level":"open_access","relation":"main_file","checksum":"e9fb251dfcb7cd209b83f17867e61321","file_id":"4842","date_updated":"2020-07-14T12:45:12Z","file_size":1308364,"creator":"system","date_created":"2018-12-12T10:10:51Z","file_name":"IST-2017-837-v1+1_ingles-prieto.pdf"}],"publication_status":"published","intvolume":" 11","month":"10","scopus_import":1,"oa_version":"Submitted Version","abstract":[{"lang":"eng"}],"department":[{"_id":"HaJa","tree":[{"_id":"ResearchGroups"},{"_id":"IST"}]},{"tree":[{"_id":"SSU"},{"_id":"IST"}],"_id":"LifeSc"}],"file_date_updated":"2020-07-14T12:45:12Z","ddc":[],"creator":{"login":"dernst","id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},"date_updated":"2023-09-07T12:49:09Z","pubrep_id":"837","status":"public","type":"journal_article","_id":"1678"}]