article published yes Álvaro Inglés Prieto author 2A9DB292-F248-11E8-B48F-1D18A9856A870000-0002-5409-8571 Eva Gschaider-Reichhart author 3FEE232A-F248-11E8-B48F-1D18A9856A870000-0002-7218-7738 Markus Muellner author Matthias Nowak author 30845DAA-F248-11E8-B48F-1D18A9856A87 Sebastian Nijman author Michael Grusch author Harald L Janovjak author 33BA6C30-F248-11E8-B48F-1D18A9856A870000-0002-8023-9315 HaJa department LifeSc department Microbial Ion Channels for Synthetic Neurobiology project In situ real-time imaging of neurotransmitter signaling using designer optical sensors project Molecular Drug Targets project High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes. https://research-explorer.ista.ac.at/download/1678/4842/IST-2017-837-v1+1_ingles-prieto.pdf application/pdfno Nature Publishing Group2015 eng 10.1038/nchembio.1933 1112952 - 954 https://research-explorer.ista.ac.at/record/418 Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. 2015;11(12):952-954. doi:<a href="https://doi.org/10.1038/nchembio.1933">10.1038/nchembio.1933</a> Á. Inglés Prieto <i>et al.</i>, “Light-assisted small-molecule screening against protein kinases,” <i>Nature Chemical Biology</i>, vol. 11, no. 12. Nature Publishing Group, pp. 952–954, 2015. Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman, S., Grusch, M., &#38; Janovjak, H. L. (2015). Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nchembio.1933">https://doi.org/10.1038/nchembio.1933</a> Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman, M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954. Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>, vol. 11, no. 12, Nature Publishing Group, 2015, pp. 952–54, doi:<a href="https://doi.org/10.1038/nchembio.1933">10.1038/nchembio.1933</a>. Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>. Nature Publishing Group, 2015. <a href="https://doi.org/10.1038/nchembio.1933">https://doi.org/10.1038/nchembio.1933</a>. Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 11(12), 952–954. 16782018-12-11T11:53:25Z2026-04-08T14:11:53Z