{"file":[{"file_id":"17393","file_name":"2022_PlantComm_Navarrete.pdf","date_updated":"2024-08-05T10:26:29Z","success":1,"date_created":"2024-08-05T10:26:29Z","content_type":"application/pdf","checksum":"1eeb6ee65419e4aa34627fea6857f343","relation":"main_file","creator":"dernst","file_size":3216686,"access_level":"open_access"}],"date_created":"2024-05-29T06:10:22Z","external_id":{"pmid":["35529945"]},"title":"TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1","publication_status":"published","oa_version":"Published Version","language":[{"iso":"eng"}],"type":"journal_article","publication":"Plant Communications","_id":"17068","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","status":"public","publisher":"Elsevier","abstract":[{"lang":"eng","text":"In plants, the antagonism between growth and defense is hardwired by hormonal signaling. The perception of pathogen-associated molecular patterns (PAMPs) from invading microorganisms inhibits auxin signaling and plant growth. Conversely, pathogens manipulate auxin signaling to promote disease, but how this hormone inhibits immunity is not fully understood. Ustilago maydis is a maize pathogen that induces auxin signaling in its host. We characterized a U. maydis effector protein, Naked1 (Nkd1), that is translocated into the host nucleus. Through its native ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif, Nkd1 binds to the transcriptional co-repressors TOPLESS/TOPLESS-related (TPL/TPRs) and prevents the recruitment of a transcriptional repressor involved in hormonal signaling, leading to the de-repression of auxin and jasmonate signaling and thereby promoting susceptibility to (hemi)biotrophic pathogens. A moderate upregulation of auxin signaling inhibits the PAMP-triggered reactive oxygen species (ROS) burst, an early defense response. Thus, our findings establish a clear mechanism for auxin-induced pathogen susceptibility. Engineered Nkd1 variants with increased expression or increased EAR-mediated TPL/TPR binding trigger typical salicylic-acid-mediated defense reactions, leading to pathogen resistance. This implies that moderate binding of Nkd1 to TPL is a result of a balancing evolutionary selection process to enable TPL manipulation while avoiding host recognition."}],"date_updated":"2024-08-05T10:27:03Z","citation":{"short":"F. Navarrete, M.C. Gallei, A.E. Kornienko, I. Saado, M. Khan, K.-S. Chia, M.A. Darino, J. Bindics, A. Djamei, Plant Communications 3 (2022).","ista":"Navarrete F, Gallei MC, Kornienko AE, Saado I, Khan M, Chia K-S, Darino MA, Bindics J, Djamei A. 2022. TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. Plant Communications. 3(2), 100269.","ieee":"F. Navarrete <i>et al.</i>, “TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1,” <i>Plant Communications</i>, vol. 3, no. 2. Elsevier, 2022.","mla":"Navarrete, Fernando, et al. “TOPLESS Promotes Plant Immunity by Repressing Auxin Signaling and Is Targeted by the Fungal Effector Naked1.” <i>Plant Communications</i>, vol. 3, no. 2, 100269, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">10.1016/j.xplc.2021.100269</a>.","ama":"Navarrete F, Gallei MC, Kornienko AE, et al. TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. <i>Plant Communications</i>. 2022;3(2). doi:<a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">10.1016/j.xplc.2021.100269</a>","apa":"Navarrete, F., Gallei, M. C., Kornienko, A. E., Saado, I., Khan, M., Chia, K.-S., … Djamei, A. (2022). TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1. <i>Plant Communications</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">https://doi.org/10.1016/j.xplc.2021.100269</a>","chicago":"Navarrete, Fernando, Michelle C Gallei, Aleksandra E. Kornienko, Indira Saado, Mamoona Khan, Khong-Sam Chia, Martin A. Darino, Janos Bindics, and Armin Djamei. “TOPLESS Promotes Plant Immunity by Repressing Auxin Signaling and Is Targeted by the Fungal Effector Naked1.” <i>Plant Communications</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.xplc.2021.100269\">https://doi.org/10.1016/j.xplc.2021.100269</a>."},"month":"03","date_published":"2022-03-14T00:00:00Z","has_accepted_license":"1","article_number":"100269","file_date_updated":"2024-08-05T10:26:29Z","acknowledgement":"The research leading to these results received funding from the European Research Council under the European Union Seventh Framework Programme ERC-2013-STG grant agreement \r\n335691; the Austrian Science Fund (FWF) P27818-B22,I 3033-B22; the Austrian Academy of Sciences (OEAW); and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2070-390732324.\r\nWe would like to thank the GMI/IMBA/IMP core facilities for excellent technical support, especially the BioOptics and Molecular Biology Services. We thank the Plant Sciences and Next Generation Sequencing Facilities at the Vienna BioCenter Core Facilities GmbH (VBCF). We are grateful to the Jirí Friml and Jürgen Kleine-Vehn laboratories for providing useful A. thaliana lines. We thank Mathias Madalinski for peptide synthesis and Dr. J. Matthew Watson for proofreading and valuable feedback on the manuscript. The authors declare no competing interests.","volume":3,"day":"14","pmid":1,"intvolume":"         3","article_processing_charge":"Yes","doi":"10.1016/j.xplc.2021.100269","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"article_type":"original","author":[{"last_name":"Navarrete","first_name":"Fernando","full_name":"Navarrete, Fernando"},{"orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","last_name":"Gallei","first_name":"Michelle C"},{"full_name":"Kornienko, Aleksandra E.","last_name":"Kornienko","first_name":"Aleksandra E."},{"last_name":"Saado","first_name":"Indira","full_name":"Saado, Indira"},{"first_name":"Mamoona","last_name":"Khan","full_name":"Khan, Mamoona"},{"full_name":"Chia, Khong-Sam","first_name":"Khong-Sam","last_name":"Chia"},{"first_name":"Martin A.","last_name":"Darino","full_name":"Darino, Martin A."},{"first_name":"Janos","last_name":"Bindics","full_name":"Bindics, Janos"},{"last_name":"Djamei","first_name":"Armin","full_name":"Djamei, Armin"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"2","quality_controlled":"1","ddc":["580"],"scopus_import":"1","year":"2022","publication_identifier":{"issn":["2590-3462"]},"department":[{"_id":"JiFr"}],"oa":1}