---
_id: '17072'
abstract:
- lang: eng
  text: The collapse of polypeptides is thought important to protein folding, aggregation,
    intrinsic disorder, and phase separation. However, whether polypeptide collapse
    is modulated in cells to control protein states is unclear. Here, using integrated
    protein manipulation and imaging, we show that the chaperonin GroEL-ES can accelerate
    the folding of proteins by strengthening their collapse. GroEL induces contractile
    forces in substrate chains, which draws them into the cavity and triggers a general
    compaction and discrete folding transitions, even for slow-folding proteins. This
    collapse enhancement is strongest in the nucleotide-bound states of GroEL and
    is aided by GroES binding to the cavity rim and by the amphiphilic C-terminal
    tails at the cavity bottom. Collapse modulation is distinct from other proposed
    GroEL-ES folding acceleration mechanisms, including steric confinement and misfold
    unfolding. Given the prevalence of collapse throughout the proteome, we conjecture
    that collapse modulation is more generally relevant within the protein quality
    control machinery.
acknowledgement: We thank A. L. Horwich, K. Chakraborty, and B. Schuler for providing
  plasmids, and R. van Leeuwen, M. Mayer, J. van Zon, W. Noorduin, and P. R. ten Wolde
  for comments and critical reading of the manuscript. Work in the group of S.J.T.
  was supported by the Netherlands Organization for Scientific Research (NWO). Work
  in the group of H.S.R. was supported by a grant from the NIH (R01GM114405).
article_number: eabl6293
article_processing_charge: Yes
article_type: original
author:
- first_name: Mohsin M.
  full_name: Naqvi, Mohsin M.
  last_name: Naqvi
- first_name: Mario
  full_name: Avellaneda Sarrió, Mario
  id: DC4BA84C-56E6-11EA-AD5D-348C3DDC885E
  last_name: Avellaneda Sarrió
  orcid: 0000-0001-6406-524X
- first_name: Andrew
  full_name: Roth, Andrew
  last_name: Roth
- first_name: Eline J.
  full_name: Koers, Eline J.
  last_name: Koers
- first_name: Antoine
  full_name: Roland, Antoine
  last_name: Roland
- first_name: Vanda
  full_name: Sunderlikova, Vanda
  last_name: Sunderlikova
- first_name: Günter
  full_name: Kramer, Günter
  last_name: Kramer
- first_name: Hays S.
  full_name: Rye, Hays S.
  last_name: Rye
- first_name: Sander J.
  full_name: Tans, Sander J.
  last_name: Tans
citation:
  ama: Naqvi MM, Avellaneda Sarrió M, Roth A, et al. Protein chain collapse modulation
    and folding stimulation by GroEL-ES. <i>Science Advances</i>. 2022;8(9). doi:<a
    href="https://doi.org/10.1126/sciadv.abl6293">10.1126/sciadv.abl6293</a>
  apa: Naqvi, M. M., Avellaneda Sarrió, M., Roth, A., Koers, E. J., Roland, A., Sunderlikova,
    V., … Tans, S. J. (2022). Protein chain collapse modulation and folding stimulation
    by GroEL-ES. <i>Science Advances</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/sciadv.abl6293">https://doi.org/10.1126/sciadv.abl6293</a>
  chicago: Naqvi, Mohsin M., Mario Avellaneda Sarrió, Andrew Roth, Eline J. Koers,
    Antoine Roland, Vanda Sunderlikova, Günter Kramer, Hays S. Rye, and Sander J.
    Tans. “Protein Chain Collapse Modulation and Folding Stimulation by GroEL-ES.”
    <i>Science Advances</i>. American Association for the Advancement of Science,
    2022. <a href="https://doi.org/10.1126/sciadv.abl6293">https://doi.org/10.1126/sciadv.abl6293</a>.
  ieee: M. M. Naqvi <i>et al.</i>, “Protein chain collapse modulation and folding
    stimulation by GroEL-ES,” <i>Science Advances</i>, vol. 8, no. 9. American Association
    for the Advancement of Science, 2022.
  ista: Naqvi MM, Avellaneda Sarrió M, Roth A, Koers EJ, Roland A, Sunderlikova V,
    Kramer G, Rye HS, Tans SJ. 2022. Protein chain collapse modulation and folding
    stimulation by GroEL-ES. Science Advances. 8(9), eabl6293.
  mla: Naqvi, Mohsin M., et al. “Protein Chain Collapse Modulation and Folding Stimulation
    by GroEL-ES.” <i>Science Advances</i>, vol. 8, no. 9, eabl6293, American Association
    for the Advancement of Science, 2022, doi:<a href="https://doi.org/10.1126/sciadv.abl6293">10.1126/sciadv.abl6293</a>.
  short: M.M. Naqvi, M. Avellaneda Sarrió, A. Roth, E.J. Koers, A. Roland, V. Sunderlikova,
    G. Kramer, H.S. Rye, S.J. Tans, Science Advances 8 (2022).
date_created: 2024-05-29T06:12:19Z
date_published: 2022-03-01T00:00:00Z
date_updated: 2024-08-05T08:30:29Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1126/sciadv.abl6293
external_id:
  pmid:
  - '35245117'
file:
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  date_created: 2024-07-31T12:01:51Z
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fulldoi: https://doi.org/10.1126/sciadv.abl6293
has_accepted_license: '1'
intvolume: '         8'
issue: '9'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
  issn:
  - 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protein chain collapse modulation and folding stimulation by GroEL-ES
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2022'
...