article published yes Giselle T Cheung author 471195F6-F248-11E8-B48F-1D18A9856A870000-0001-8457-2572 Carmen Streicher author 36BCB99C-F248-11E8-B48F-1D18A9856A87 Simon Hippenmeyer author 37B36620-F248-11E8-B48F-1D18A9856A870000-0003-2279-1061 SiHi department ISTplus - Postdoctoral Fellowships project Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular Mechanisms of Neural Stem Cell Lineage Progression project The generation of diverse cell types during development is fundamental to brain functions. We outline a protocol to quantitatively assess the clonal output of individual neural progenitors using mosaic analysis with double markers (MADM) in mice. We first describe steps to acquire and reconstruct adult MADM clones in the superior colliculus. Then we detail analysis pipelines to determine clonal composition and architecture. This protocol enables the buildup of quantitative frameworks of lineage progression with precise spatial resolution in the brain. For complete details on the use and execution of this protocol, please refer to Cheung et al.1 https://research-explorer.ista.ac.at/download/17187/18809/2024_STARProtoc_Cheung.pdf application/pdfno https://creativecommons.org/licenses/by-nc-nd/4.0/ Elsevier2024 eng 2666-1667 3893550810.1016/j.xpro.2024.103157 53 G.T. Cheung, C. Streicher, S. Hippenmeyer, STAR Protocols 5 (2024). Cheung, Giselle T., et al. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” <i>STAR Protocols</i>, vol. 5, no. 3, 103157, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xpro.2024.103157">10.1016/j.xpro.2024.103157</a>. Cheung, G. T., Streicher, C., &#38; Hippenmeyer, S. (2024). Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2024.103157">https://doi.org/10.1016/j.xpro.2024.103157</a> Cheung, Giselle T, Carmen Streicher, and Simon Hippenmeyer. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2024.103157">https://doi.org/10.1016/j.xpro.2024.103157</a>. Cheung GT, Streicher C, Hippenmeyer S. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. <i>STAR Protocols</i>. 2024;5(3). doi:<a href="https://doi.org/10.1016/j.xpro.2024.103157">10.1016/j.xpro.2024.103157</a> Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 5(3), 103157. G. T. Cheung, C. Streicher, and S. Hippenmeyer, “Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice,” <i>STAR Protocols</i>, vol. 5, no. 3. Elsevier, 2024. 171872024-06-30T22:01:04Z2025-12-30T10:54:11Z