---
APC_amount: 804 EUR
OA_place: publisher
OA_type: gold
_id: '17187'
abstract:
- lang: eng
  text: "The generation of diverse cell types during development is fundamental to
    brain\r\nfunctions. We outline a protocol to quantitatively assess the clonal
    output of individual neural progenitors using mosaic analysis with double markers
    (MADM) in\r\nmice. We first describe steps to acquire and reconstruct adult MADM
    clones in\r\nthe superior colliculus. Then we detail analysis pipelines to determine
    clonal\r\ncomposition and architecture. This protocol enables the buildup of quantitative\r\nframeworks
    of lineage progression with precise spatial resolution in the brain.\r\nFor complete
    details on the use and execution of this protocol, please refer to\r\nCheung et
    al.1"
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank A. Heger for mouse breeding support. This work was supported
  by the Scientific Service Units of IST Austria through resources provided by the
  Imaging & Optics and Preclinical facilities. G.C. received funding from the European
  Commission (IST plus postdoctoral fellowship); S.H. was funded by ISTA institutional
  funds and the Austrian Science Fund Special Research Programmes (FWF SFB-F78 Neuro
  Stem Modulation).
article_number: '103157'
article_processing_charge: Yes
article_type: original
author:
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: Cheung GT, Streicher C, Hippenmeyer S. Protocol for quantitative reconstruction
    of cell lineage using mosaic analysis with double markers in mice. <i>STAR Protocols</i>.
    2024;5(3). doi:<a href="https://doi.org/10.1016/j.xpro.2024.103157">10.1016/j.xpro.2024.103157</a>
  apa: Cheung, G. T., Streicher, C., &#38; Hippenmeyer, S. (2024). Protocol for quantitative
    reconstruction of cell lineage using mosaic analysis with double markers in mice.
    <i>STAR Protocols</i>. Elsevier. <a href="https://doi.org/10.1016/j.xpro.2024.103157">https://doi.org/10.1016/j.xpro.2024.103157</a>
  chicago: Cheung, Giselle T, Carmen Streicher, and Simon Hippenmeyer. “Protocol for
    Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double
    Markers in Mice.” <i>STAR Protocols</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xpro.2024.103157">https://doi.org/10.1016/j.xpro.2024.103157</a>.
  ieee: G. T. Cheung, C. Streicher, and S. Hippenmeyer, “Protocol for quantitative
    reconstruction of cell lineage using mosaic analysis with double markers in mice,”
    <i>STAR Protocols</i>, vol. 5, no. 3. Elsevier, 2024.
  ista: Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction
    of cell lineage using mosaic analysis with double markers in mice. STAR Protocols.
    5(3), 103157.
  mla: Cheung, Giselle T., et al. “Protocol for Quantitative Reconstruction of Cell
    Lineage Using Mosaic Analysis with Double Markers in Mice.” <i>STAR Protocols</i>,
    vol. 5, no. 3, 103157, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xpro.2024.103157">10.1016/j.xpro.2024.103157</a>.
  short: G.T. Cheung, C. Streicher, S. Hippenmeyer, STAR Protocols 5 (2024).
corr_author: '1'
date_created: 2024-06-30T22:01:04Z
date_published: 2024-09-20T00:00:00Z
date_updated: 2025-12-30T10:54:11Z
day: '20'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.xpro.2024.103157
ec_funded: 1
external_id:
  pmid:
  - '38935508'
file:
- access_level: open_access
  checksum: d8a8cdba82a394e731aa699ace1ae433
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-09T12:12:40Z
  date_updated: 2025-01-09T12:12:40Z
  file_id: '18809'
  file_name: 2024_STARProtoc_Cheung.pdf
  file_size: 5186071
  relation: main_file
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file_date_updated: 2025-01-09T12:12:40Z
has_accepted_license: '1'
intvolume: '         5'
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F7805
  name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
    Mechanisms of Neural Stem Cell Lineage Progression
publication: STAR Protocols
publication_identifier:
  eissn:
  - 2666-1667
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Protocol for quantitative reconstruction of cell lineage using mosaic analysis
  with double markers in mice
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...