---
_id: '1727'
abstract:
- lang: eng
  text: 'Cells at different positions in a developing tissue receive different concentrations
    of signaling molecules, called morphogens, and this influences their cell fate.
    Morphogen concentration gradients have been proposed to control patterning as
    well as growth in many developing tissues. Some outstanding questions about tissue
    patterning by morphogen gradients are the following: What are the mechanisms that
    regulate gradient formation and shape? Is the positional information encoded in
    the gradient sufficiently precise to determine the positions of target gene domain
    boundaries? What are the temporal dynamics of gradients and how do they relate
    to patterning and growth? These questions are inherently quantitative in nature
    and addressing them requires measuring morphogen concentrations in cells, levels
    of downstream signaling activity, and kinetics of morphogen transport. Here we
    first present methods for quantifying morphogen gradient shape in which the measurements
    can be calibrated to reflect actual morphogen concentrations. We then discuss
    using fluorescence recovery after photobleaching to study the kinetics of morphogen
    transport at the tissue level. Finally, we present particle tracking as a method
    to study morphogen intracellular trafficking.'
author:
- first_name: Anna
  full_name: Anna Kicheva
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
- first_name: Laurent
  full_name: Holtzer, Laurent
  last_name: Holtzer
- first_name: Ortrud
  full_name: Wartlick, Ortrud
  last_name: Wartlick
- first_name: Thomas
  full_name: Schmidt, Thomas S
  last_name: Schmidt
- first_name: Marcos
  full_name: González-Gaitán, Marcos A
  last_name: González Gaitán
citation:
  ama: Kicheva A, Holtzer L, Wartlick O, Schmidt T, González Gaitán M. Quantitative
    imaging of morphogen gradients in drosophila imaginal discs. <i>Cold Spring Harbor
    Protocols</i>. 2013;8(5):387-403. doi:<a href="https://doi.org/10.1101/pdb.top074237">10.1101/pdb.top074237</a>
  apa: Kicheva, A., Holtzer, L., Wartlick, O., Schmidt, T., &#38; González Gaitán,
    M. (2013). Quantitative imaging of morphogen gradients in drosophila imaginal
    discs. <i>Cold Spring Harbor Protocols</i>. Cold Spring Harbor Laboratory Press.
    <a href="https://doi.org/10.1101/pdb.top074237">https://doi.org/10.1101/pdb.top074237</a>
  chicago: Kicheva, Anna, Laurent Holtzer, Ortrud Wartlick, Thomas Schmidt, and Marcos
    González Gaitán. “Quantitative Imaging of Morphogen Gradients in Drosophila Imaginal
    Discs.” <i>Cold Spring Harbor Protocols</i>. Cold Spring Harbor Laboratory Press,
    2013. <a href="https://doi.org/10.1101/pdb.top074237">https://doi.org/10.1101/pdb.top074237</a>.
  ieee: A. Kicheva, L. Holtzer, O. Wartlick, T. Schmidt, and M. González Gaitán, “Quantitative
    imaging of morphogen gradients in drosophila imaginal discs,” <i>Cold Spring Harbor
    Protocols</i>, vol. 8, no. 5. Cold Spring Harbor Laboratory Press, pp. 387–403,
    2013.
  ista: Kicheva A, Holtzer L, Wartlick O, Schmidt T, González Gaitán M. 2013. Quantitative
    imaging of morphogen gradients in drosophila imaginal discs. Cold Spring Harbor
    Protocols. 8(5), 387–403.
  mla: Kicheva, Anna, et al. “Quantitative Imaging of Morphogen Gradients in Drosophila
    Imaginal Discs.” <i>Cold Spring Harbor Protocols</i>, vol. 8, no. 5, Cold Spring
    Harbor Laboratory Press, 2013, pp. 387–403, doi:<a href="https://doi.org/10.1101/pdb.top074237">10.1101/pdb.top074237</a>.
  short: A. Kicheva, L. Holtzer, O. Wartlick, T. Schmidt, M. González Gaitán, Cold
    Spring Harbor Protocols 8 (2013) 387–403.
date_created: 2018-12-11T11:53:41Z
date_published: 2013-05-01T00:00:00Z
date_updated: 2021-01-12T06:52:47Z
day: '01'
doi: 10.1101/pdb.top074237
extern: 1
intvolume: '         8'
issue: '5'
month: '05'
page: 387 - 403
publication: Cold Spring Harbor Protocols
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '5401'
quality_controlled: 0
status: public
title: Quantitative imaging of morphogen gradients in drosophila imaginal discs
type: journal_article
volume: 8
year: '2013'
...