---
APC_amount: 12348 EUR
OA_place: publisher
OA_type: hybrid
_id: '17460'
abstract:
- lang: eng
text: Filaments in the cell commonly treadmill. Driven by energy consumption, they
grow on one end while shrinking on the other, causing filaments to appear motile
even though individual proteins remain static. This process is characteristic
of cytoskeletal filaments and leads to collective filament self-organization.
Here we show that treadmilling drives filament nematic ordering by dissolving
misaligned filaments. Taking the bacterial FtsZ protein involved in cell division
as an example, we show that this mechanism aligns FtsZ filaments in vitro and
drives the organization of the division ring in living Bacillus subtilis cells.
We find that ordering via local dissolution also allows the system to quickly
respond to chemical and geometrical biases in the cell, enabling us to quantitatively
explain the ring formation dynamics in vivo. Beyond FtsZ and other cytoskeletal
filaments, our study identifies a mechanism for self-organization via constant
birth and death of energy-consuming filaments.
acknowledgement: We thank I. Palaia (ISTA) for useful discussions and K. Lim and R.
W. Wong (WPI-Nano Life Science Institute, Kanazawa University) for providing access
to HS-AFM. We would like to thank B. Prats Mateu (MSD Austria, Vienna) for providing
the HS-AFM data. This work was supported by the Royal Society (grant no. UF160266;
C.V.-C. and A.Š.), the European Union’s Horizon 2020 Research and Innovation Programme
(grant no. 802960; A.Š.), the Austrian Science Fund (FWF) Stand-Alone P34607 (M.L.)
and a Wellcome Trust and Royal Society Sir Henry Dale Fellowship (grant no. 206670/Z/17/Z;
S.H. and K.D.W.).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Christian Eduardo
full_name: Vanhille-Campos, Christian Eduardo
id: 3adeca52-9313-11ed-b1ac-c170b2505714
last_name: Vanhille-Campos
- first_name: Kevin D.
full_name: Whitley, Kevin D.
last_name: Whitley
- first_name: Philipp
full_name: Radler, Philipp
id: 40136C2A-F248-11E8-B48F-1D18A9856A87
last_name: Radler
orcid: '0000-0001-9198-2182 '
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Séamus
full_name: Holden, Séamus
last_name: Holden
- first_name: Anđela
full_name: Šarić, Anđela
id: bf63d406-f056-11eb-b41d-f263a6566d8b
last_name: Šarić
orcid: 0000-0002-7854-2139
citation:
ama: Vanhille-Campos CE, Whitley KD, Radler P, Loose M, Holden S, Šarić A. Self-organization
of mortal filaments and its role in bacterial division ring formation. Nature
Physics. 2024;20:1670-1678. doi:10.1038/s41567-024-02597-8
apa: Vanhille-Campos, C. E., Whitley, K. D., Radler, P., Loose, M., Holden, S.,
& Šarić, A. (2024). Self-organization of mortal filaments and its role in
bacterial division ring formation. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-024-02597-8
chicago: Vanhille-Campos, Christian Eduardo, Kevin D. Whitley, Philipp Radler, Martin
Loose, Séamus Holden, and Anđela Šarić. “Self-Organization of Mortal Filaments
and Its Role in Bacterial Division Ring Formation.” Nature Physics. Springer
Nature, 2024. https://doi.org/10.1038/s41567-024-02597-8.
ieee: C. E. Vanhille-Campos, K. D. Whitley, P. Radler, M. Loose, S. Holden, and
A. Šarić, “Self-organization of mortal filaments and its role in bacterial division
ring formation,” Nature Physics, vol. 20. Springer Nature, pp. 1670–1678,
2024.
ista: Vanhille-Campos CE, Whitley KD, Radler P, Loose M, Holden S, Šarić A. 2024.
Self-organization of mortal filaments and its role in bacterial division ring
formation. Nature Physics. 20, 1670–1678.
mla: Vanhille-Campos, Christian Eduardo, et al. “Self-Organization of Mortal Filaments
and Its Role in Bacterial Division Ring Formation.” Nature Physics, vol.
20, Springer Nature, 2024, pp. 1670–78, doi:10.1038/s41567-024-02597-8.
short: C.E. Vanhille-Campos, K.D. Whitley, P. Radler, M. Loose, S. Holden, A. Šarić,
Nature Physics 20 (2024) 1670–1678.
corr_author: '1'
date_created: 2024-08-25T22:01:08Z
date_published: 2024-10-01T00:00:00Z
date_updated: 2025-09-08T09:02:20Z
day: '01'
ddc:
- '570'
department:
- _id: AnSa
- _id: MaLo
doi: 10.1038/s41567-024-02597-8
ec_funded: 1
external_id:
isi:
- '001289394500005'
pmid:
- '39416851'
file:
- access_level: open_access
checksum: c4842152e2b90d67f48ea8c9ed7c473b
content_type: application/pdf
creator: dernst
date_created: 2025-04-14T06:06:35Z
date_updated: 2025-04-14T06:06:35Z
file_id: '19556'
file_name: 2024_NaturePhysics_VanhilleCampos.pdf
file_size: 8058249
relation: main_file
success: 1
file_date_updated: 2025-04-14T06:06:35Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 1670-1678
pmid: 1
project:
- _id: fc38323b-9c52-11eb-aca3-ff8afb4a011d
grant_number: P34607
name: In vitro reconstitution of bacterial cell division
- _id: eba2549b-77a9-11ec-83b8-a81e493eae4e
call_identifier: H2020
grant_number: '802960'
name: 'Non-Equilibrium Protein Assembly: from Building Blocks to Biological Machines'
publication: Nature Physics
publication_identifier:
eissn:
- 1745-2481
issn:
- 1745-2473
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Self-organization of mortal filaments and its role in bacterial division ring
formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 20
year: '2024'
...