---
res:
  bibo_abstract:
  - Sodium dodecyl sulfate-digested freeze-fracture replica labeling (SDS-FRL) is
    an electron microscope (EM) sample preparation technique which allows for high-resolution
    visualization of membrane proteins with high sensitivity. However, image acquisition
    of specific replica profiles such as synapses in a large field of EM view needs
    a valid experience and a long time for manual searching. Here, we describe how
    to utilize deep learning for automatizing image acquisition of specific profiles
    of interest in replica samples. This protocol facilitates the labor-intensive
    collection of EM images, in particular for rare profiles. We provide instructions
    for using SerialEM image acquisition software in conjunction with object detection
    by our newly developed deep learning software DarEM, to automatically acquire
    tilt series of all synapses in a selected region. We then show how to perform
    a mostly automated analysis of gold particle labeling in the acquired images by
    utilizing Darea software.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: David
      foaf_name: Kleindienst, David
      foaf_surname: Kleindienst
      foaf_workInfoHomepage: http://www.librecat.org/personId=42E121A4-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Tommaso
      foaf_name: Costanzo, Tommaso
      foaf_surname: Costanzo
      foaf_workInfoHomepage: http://www.librecat.org/personId=D93824F4-D9BA-11E9-BB12-F207E6697425
    orcid: 0000-0001-9732-3815
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  bibo_doi: 10.1007/978-1-0716-4019-7_8
  dct_date: 2024^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0893-2336
  - http://id.crossref.org/issn/1940-6045
  - http://id.crossref.org/issn/9781071640180
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica
    Samples with Deep Learning@
...