{"_id":"18058","citation":{"chicago":"Watson, Jake, Sandra Arroyo-Urea, and Javier García-Nafría. “DNA Cloning.” In <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., 66–72. Boca Raton: CRC Press, 2024. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>.","ieee":"J. Watson, S. Arroyo-Urea, and J. García-Nafría, “DNA Cloning,” in <i>Handbook of Molecular Biotechnology</i>, 1st ed., D. Liu, Ed. Boca Raton: CRC Press, 2024, pp. 66–72.","ista":"Watson J, Arroyo-Urea S, García-Nafría J. 2024.DNA Cloning. In: Handbook of Molecular Biotechnology. , 66–72.","ama":"Watson J, Arroyo-Urea S, García-Nafría J. DNA Cloning. In: Liu D, ed. <i>Handbook of Molecular Biotechnology</i>. 1st ed. Boca Raton: CRC Press; 2024:66-72. doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>","short":"J. Watson, S. Arroyo-Urea, J. García-Nafría, in:, D. Liu (Ed.), Handbook of Molecular Biotechnology, 1st ed., CRC Press, Boca Raton, 2024, pp. 66–72.","apa":"Watson, J., Arroyo-Urea, S., &#38; García-Nafría, J. (2024). DNA Cloning. In D. Liu (Ed.), <i>Handbook of Molecular Biotechnology</i> (1st ed., pp. 66–72). Boca Raton: CRC Press. <a href=\"https://doi.org/10.1201/9781003055211-8\">https://doi.org/10.1201/9781003055211-8</a>","mla":"Watson, Jake, et al. “DNA Cloning.” <i>Handbook of Molecular Biotechnology</i>, edited by Dongyou Liu, 1st ed., CRC Press, 2024, pp. 66–72, doi:<a href=\"https://doi.org/10.1201/9781003055211-8\">10.1201/9781003055211-8</a>."},"title":"DNA Cloning","publication_identifier":{"eisbn":["9781003055211"]},"place":"Boca Raton","quality_controlled":"1","date_updated":"2024-09-11T11:16:58Z","scopus_import":"1","editor":[{"last_name":"Liu","full_name":"Liu, Dongyou","first_name":"Dongyou"}],"month":"09","abstract":[{"lang":"eng","text":"DNA cloning is a core technique in biomedical and biotechnological research and is used to assemble and modify DNA fragments at will. While DNA cloning has traditionally relied on restriction enzymes, recent homology-based methods offer improved protocols together with seamless and directional assembly of desired products, overcoming the main disadvantages of restriction enzyme DNA cloning. This chapter provides a historical perspective on DNA cloning, presents a detailed discussion on state-of-the-art in vitro and in vivo homology-based methodologies, covering the basics of how to perform all major plasmid modifications (sub-cloning, site-directed mutagenesis, insertions, and deletions), and gives examples of how to apply these techniques for complex DNA cloning projects."}],"author":[{"orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E","last_name":"Watson","full_name":"Watson, Jake","first_name":"Jake"},{"first_name":"Sandra","last_name":"Arroyo-Urea","full_name":"Arroyo-Urea, Sandra"},{"full_name":"García-Nafría, Javier","last_name":"García-Nafría","first_name":"Javier"}],"date_created":"2024-09-11T10:40:36Z","article_processing_charge":"No","date_published":"2024-09-05T00:00:00Z","department":[{"_id":"PeJo"}],"oa_version":"None","type":"book_chapter","language":[{"iso":"eng"}],"status":"public","edition":"1","publisher":"CRC Press","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"66-72","day":"05","year":"2024","publication":"Handbook of Molecular Biotechnology","doi":"10.1201/9781003055211-8"}